Short Article - Journal of Clinical and Bioanalytical Chemistry (2021) Volume 5, Issue 1

High Performance Liquid Chromatographic Determination of Ciprofloxacin Hydrochloride and Ornidazole in Human Plasma

A simple, rapid, sensitive selective chromatographic method has been developed for simultaneous determination of ciprofloxacin hydrochloride and ornidazole in human plasma by using internal standard. The method depends on reverse phase high performance liquid chromatography. The plasma sample was extracted using methanol: formic acid (5.5:0.5, v/v). A concentration range from 100-400 ng/ml for both drugs was used for calibration curve. The percent recoveries of ciprofloxacin and ornidazole were found to be 71.49-75.68 and 73.78-83.1 respectively. The mobile used consist of acetonitrile: methanol: water: triethylamine (40:20:40: 1% v/v/v) and flow rate 1 ml/min in isocratic mode. The separation was carried out by UV detector at wavelength 300 nm. The stability of ciprofloxacin hydrochloride and ornidazole in plasma were confirmed during three freeze-thaw cycles (-20°C), on bench during 12h, and post preparative stability study. The proposed method was validated statistically and by performing recoveries study for determination of ciprofloxacin hydrochloride and ornidazole in human plasma. Ciprofloxacin [1-cyclopropyl-6-fluoro-1,4-dihydro-4- oxo-7-(piperazinyl)-quinolone-3-carboxylic acid] is broad spectrum fluoroquinolone antibacterial agent. It is effective in the treatment of a wide variety of infections including infections of bones and joints, particularly those caused by gram-negative pathogens. Grampositive bacteria are generally susceptible or moderately susceptible.

Author(s): AR Rote

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