Asian Journal of Biomedical and Pharmaceutical Sciences

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Research Article - Asian Journal of Biomedical and Pharmaceutical Sciences (2017) Volume 7, Issue 63

Hepatoprotective and Antioxidant Effect of Stem Barks Extracts: Methanolic and Aqueous of Pseudocedrela kotschyi (Meliaceae) on Paracetamol-Induced Hepatic Damage in Rats.

The present work has been designed to evaluate hepatoprotective and in vivo antioxidant effects of aqueous and methanolic of the stem barks extracts of Pseudocedrela kotschyi against paracetamol induced hepatotoxicity in experimental rats. Hepatic damage was induced by oral administration of paracetamol (2 g/kg) in rats after two weeks of pretreatment with plants extracts. The levels of transaminases (ALT and AST), total bilirubin (TB), alkaline phosphatase (ALP), Plasma total protein (TP), total cholesterol and HDL-Cholesterol were used to assess the integrity of the liver. Antioxidant status in liver was determined by measuring the activity of malonaldehyde (MDA), glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT). Histopathological study of isolated liver specimen was also carried out. The oral pre-treatment of rats with aqueous and methanolic extracts (50, 100 and 150 mg/kg bw) showed significant (p?0.001) hepatoprotective activity against paracetamol induced hepatotoxicity by decreasing the activities of transaminases (ALT and AST), total bilirubin, alkaline phosphatase, total cholesterol and MDA compared to negative control. It increase significantly (p?0.001) the plasma protein concentration, HDL-cholesterol, liver homogenate of GSH, CAT and SOD activities compared to negative control. The effects of aqueous and methanolic extracts were comparable to a standard drug, silymarin, which was confirmed by histopathological examinations where plants extracts reduced progressively the hepatic damage in rats. These findings, therefore supported the traditional believes on hepatoprotective effect of the Pseudocedrela kotschyi stem barks extracts.

Author(s): Moise Legentil Nchouwet, Sylvie Lea Wansi Ngnokam, Norbert Kodjio, Sylviane Kamani Poualeu, Pepin Alango Nkengeffouet, Albert Kamanyi

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