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Review Article - Asian Journal of Biomedical and Pharmaceutical Sciences (2021) Volume 11, Issue 79
Emerging functional assays used for vaccine efficiency.
In the countries of the west, meningococcal disease is frequently caused by Neisseria meningitidis. Major to increase a secure and efficacious vaccine towards this serogroup which is appropriate for use in infants and younger children. After vaccination with candidate vaccines, to determine the high- quality of the immune response laboratory correlates of safety are needed. For serogroups A and C, Serum Bactericidal Assay (SBA) is a well-set up predictor for safety, however for serogroup B different mechanisms besides SBA can also be worried in conferring safety from disease. The general SBAs are labor intensive and the variations in protocols among specific laboratories make interpretation of outcomes difficult. Hence, the SBA assays with a colorimetric and a fluorometric end point within the presence of aamarblue were selected and compared to a well-known conventional SBA assay, wherein colony counts are performed to evaluate the titer. A colorimetric end point required a spectrophotometer, while a fluorometric end point required a fluorometer. Several laboratory strategies for recognition and evaluation of the immunogenicity of possible vaccine antigens are described in this review.
Author(s): Pratik Zagade, Nigade