Gynecology and Reproductive Endocrinology

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Special Issue Article - Gynecology and Reproductive Endocrinology (2021) Volume 5, Issue 4

Effect of the hydroalcoholic extract from Lampaya medicinalis Phil. (Verbenaceae) on palmitic acid- impaired insulin signaling in adipocytes

Female obesity is associated with menstrual disorders, infertility and recurrent miscarriages (1). Additionally, obesity is linked to metabolic pathologies such as dyslipidemia, insulin resistance (IR) and type 2 diabetes mellitus (T2DM). Altered insulin function has been associated with abnormalities in female reproduction (2). Palmitic acid (PA) is a saturated fatty acid with well-known IRinducing effects in vivo and in vitro (3). Lampaya medicinalis Phil. (Verbenaceae) is a small bush that grows in the North of Chile. The infusion of the plant has been used by local ethnic groups to treat and cure diseases (4). The aim of this study was to assess in vitro the effect of the hydroalcoholic extract of Lampaya medicinalis (HEL) against PA-induced IR in adipocytes from the 3T3-L1 cell line (5). Methods: 3T3-L1 cells were in vitro-differentiated to adipocytes using a standard adipogenic cocktail. Cytotoxicity of a range of HEL concentrations (0.01 to 10 μg/ml) was evaluated in adipocytes. Cells were exposed or not to 0.1 μg/mL of HEL before adding 0.65 mM PA or vehicle and incubated with 100 nM insulin (or vehicle) for 15 min. Phosphorylation of insulin-signaling molecules (Tyr-IRS-1, Ser-Akt, Thr-AS160) was evaluated by Western blot. Glucose uptake was assessed using the 2-NBDG analogue. Results: HEL was not cytotoxic at any concentration assessed. PA-treated adipocytes showed an impairment in insulinstimulated phosphorylation of Tyr-IRS-1, Ser-Akt and Thr-AS160 compared to control (p<0.05). Interestingly, when HEL was present, proteins phosphorylation increased significantly (p<0.05) with respect to PA-treated adipocytes. Conclusion: These findings give new insights about the effect of HEL ameliorating PA- impaired IRS-1/Akt/AS160 pathway and glucose uptake in adipocytes (5). A protective role for HEL against PA-induced IR is suggested, therefore Lampaya medicinalis may represent a promising therapeutic tool for adipocyte IR, a key component in whole body IR which is associated with impaired reproductive health

Author(s): Paulina Ormazabal

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