Biomedical Research

Research Article - Biomedical Research (2018) Volume 29, Issue 19

Effects of quail (Coturnix japonica) egg diet on both the blood sugar and the lipid profile of alloxan induced diabetic albino rats

Umera AE1, Ejezie FE1, Ibegbu MD1*, Ikekpeazu JE1, Onyekwelu KC1 and Ejezie CS2

1Department of Medical Biochemistry, College of Medicine, University of Nigeria, Enugu Campus (UNEC), Enugu, Nigeria

2Department of Haematology and Immunology, College of Medicine, Ituku-Ozalla, Nigeria

*Corresponding Author:
Ibegbu MD
Department of Medical Biochemistry
College of Medicine
University of Nigeria, Enugu Campus (UNEC)
Nigeria

Accepted date: September 24, 2018

DOI: 10.4066/biomedicalresearch.29-18-989

Visit for more related articles at Biomedical Research

Abstract

Quail eggs have been found useful in folk medicine in treatment of some diseases, but its consumption is not as common as chicken eggs amongst humans. This study was aimed at determining the dietary effects of quail eggs on blood sugar and lipid profile of alloxan induced diabetic rats. Quail egg samples were analysed for its various nutritional compositions using the Association of Official Analytic Chemistry (AOAC) methods. Quail eggs were administered to thirty six (36) alloxan induced diabetic rats, which were divided into nine (9) different groups of four (4) rats each per group at varied doses for a duration of seven (7), fourteen (14) and twenty one (21) days. Their blood sugar and lipid profile were determined using standard methods. Proximate analysis showed that quail egg could be a good source of protein, lipid and has high moisture content while it has minimal ash and carbohydrate contents. Rats treated with two (2) raw quail eggs showed the highest blood glucose lowering capacity and weight gain when compared with the insulin treated rats. Evaluation of the blood glucose at intervals (d 7, d 14, and d 21) showed that quail eggs could serve for mid-term and long term treatment of diabetes, but did not have any significant effects on the serum lipid profile of diabetic rats. It could be concluded that intake of quail egg diets either alone or as part of a therapeutic regime, could have beneficial effect in the prevention and management of diabetes mellitus.

Keywords

Quail eggs, Diabetes, Blood sugar, Lipid profile

Introduction

In human body, a regular energy source is a prerequisite for normal cell function. Glucose is the primary energy source of the body, which circulates in the blood as a mobilizable fuel source for cells [1,2]. Diabetes mellitus is a group of metabolic disorders characterized by high level of glucose in the blood resulting from defects in insulin secretion and/or increased cellular resistance to insulin [3]. Chronic hyperglycaemia and other metabolic disturbances of diabetes mellitus lead to longterm tissue and organ damage as well as dysfunction involving the eyes, kidneys, nervous and vascular systems [4].

Avian eggs are an inexpensive and highly nutritious food, providing 13 vitamins and 7 minerals [5], the composition of which can be affected by several factors such as diet, age, strain as well as environmental factors [6]. The eggs are conventional food containing nutrients that play fundamental roles beyond basic nutrition [7]. Eggs are of particular interest from a functionality point of view because they are excellent source of protein, moderate calorie, also inexpensive, these makes eggs affordable to good percentage of many populations [8]. However, eggs are a controversial food for nutritional experts and health agencies because of the saturated fat and cholesterol content [9]. This was based on the assumption that high dietary cholesterol consumption is associated with high blood cholesterol levels and cardiovascular disease [10].

Quail eggs are used in folk medicine. For instance, Chinese use quail eggs in the treatment of tuberculosis, asthma and diabetes and helps prevent kidney, liver, or gallbladder stones and even remove these types of stones [11]. In Japan, consumption of Japanese quail eggs has been claimed to improve metabolism; prevent stress; and help in the treatment of obesity, asthma, and various allergies [12]. In Brazil, there is a claim that it relieves many physiological disorders such as anaemia, tuberculosis, ulcers, hypertension, diabetes, arteriosclerosis, asthma and many other diseases [13]. Regular consumption of quail eggs has been shown to fight against many diseases, which includes digestive tract disorders such as stomach ulcers, it strengthens the immune system, promotes memory health, increases brain activity and stabilizes the nervous system. This also is beneficial in anaemia management by increasing the level of haemoglobin in the body while removing toxins and heavy metals. The nutritional value of quail eggs is higher than those offered by other eggs in being a rich sources of antioxidants, minerals, and vitamins [14]. The nutritional value of quail eggs is 3-4 times greater than chicken eggs and studies have shown that the shell of quail eggs consists of 90% calcium and in addition contains minerals like copper, fluorine, sulphur, silicon, zinc and stimulates hematopoietic function of bone marrow [15].

With the increasing search for natural remedies to diseases especially diabetes mellitus, this study was initiated to ascertain the effect of quail eggs on the blood sugar and lipid profile of alloxan induced diabetic albino rats.

Materials and Methods

Sample collection

Quail eggs (Coturnix coturnix japonica) were purchased from a poultry House in Enugu, Nigeria. For the purpose of this study 60 eggs were chosen randomly and four (4) were randomly selected and weighed to obtain an estimated average weight of each quail egg. The egg samples were carefully handled in an egg-box and transported to the laboratory.

Proximate and elemental analysis

The protein, fat and moisture content were evaluated using the method of the Association of Official Analytical Chemists [16]. The elemental analysis of the eggshell and the egg was done as described by Allen [17].

Processing of eggs and egg shells for feeding of rats

The de-shelled quail eggs were put in a non-stick frying pan and cooked for about 5 min. The cooked egg was then placed into a drying rack in dehydrator and oven-dried for around 16-18 h at 70°C until completely brittle substance was obtained, which was pulverised into powder with blender and stored for use.

The egg shells were properly washed with distilled water in order to remove all adjacent egg content, oven dried for between 6-8 h at 70°C until completely brittle, and pulverised into powder and stored for use.

Animal experiment

This study was approved by the Faculty of Basic Medical Sciences animal research ethics committee. Thirty six (36) male albino rats weighing about 240 g were used for the experiment. The animals were purchased from Department of Human Physiology, University of Nigeria, Enugu Campus. The rats were allowed to acclimatize for 14 d and were assigned into nine groups with four rats each per group as described below. The rats were induced with diabetes and were maintained at 25 ± 2°C in a room with a 12/12 h light/dark cycle and fed with standard rat chow and quail egg. Rats in groups 1-3 received standard rat chow while the rest received quail egg diet in addition.

Administration of quail egg diet, insulin/collection of blood samples

After diabetes induction, the rats of groups 4-9 were treated with different preparations of quail egg diet for 21 d as described below; while insulin (regular insulin) 40 IU/ml solution per 100 g body weight was administered daily for 21 d to group 3 rats.

Group Treatment
1  Normal control rats (NC)
2  Diabetic Non-treated Rats (DNT)
3  Diabetic rats treated with insulin (DTI)
4  Diabetic rats treated with two (2) processed Quail eggs (2PE)
5  Diabetic Rats treated with three (3) processed Quail eggs (3PE)
6  Diabetic Rats treated with two processed Quail eggs and two processed shells (2PES)
7  Diabetic Rats treated with three processed Quail eggs and three processed shells (3PES)
8  Diabetic rats treated with two (2) raw Quail eggs (2RE)
9  Diabetic rats treated with three (3) raw Quail eggs (3RE)

Induction of diabetes

The baseline blood glucose levels of the rats were determined before diabetes induction. Diabetes was induced on the rats by intra-peritoneal (IP) injection of 150 mg/kg body weight of alloxan monohydrate solution [18].

Sample analyses

The blood glucose levels of the rats were determined using glucometer. The body weights of the rats before induction, after induction and at intervals during the feed administration were recorded. The blood glucose levels of the rats were determined on d 1, 7, 14 and 21 during the administration of the processed and raw quail egg diets. After 21 d, the rats were sacrificed and the blood samples collected for glucose estimation, also serum prepared from the blood samples for lipid profile analysis. total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) were determined using Hitachi 704 Analyzer according to the manufacturer’s instruction.

Data analysis

The Statistical Package for Social Science (SPSS) computer software version 17 was used for data analysis. The results of the tests were analysed using analysis of variance (ANOVA) and student’s t-test at 95% confidence interval with p value of ≤ 0.05 been considered as significant.

Results

The results of the proximate composition revealed that the quail eggs were good sources of protein, lipids and with high moisture content. However, the ash and carbohydrate contents were minimal (Table 1).

Parameter Value (g/100g)
Albumen Yolk
Protein 9.4 ± 0.04 15.10 ± 0.16
Lipid content 0.41 ± 0.05 31.39 ± 0.26
Ash content 1.13 ± 0.09 1.99 ± 0.09
Moisture 88.48 ± 0.36 50.18 ± 0.25
Carbohydrate 0.65 ± 0.05 1.08 ± 0.25

Table 1. Proximate composition of Quail egg, Coturnix japonica.

The evaluation of elemental composition revealed that the whole egg (both the egg white and yoke) was rich in calcium (150.50 ± 0.71 mg/100 g), zinc (43.73 ± 0.04 mg/100 g), iron (78.90 ± 0.14 mg/100 g), phosphorous (120.56 ± 0.62 mg/100 g) and magnesium (105.50 ± 0.71 mg/100 g while the shell also revealed that it was equally rich in calcium (300.25 ± 0.35 mg/100 g), zinc (38.13 ± 0.88 mg/100 g), Iron (175.70 ± 0.42 mg/100 g) and magnesium (78.15 ± 0.21 mg/100 g). However, the copper, potassium and manganese contents were minimal (Table 2).

Parameter Values (μg/g or mg/l)
Whole egg Shell
Calcium (Ca) 1500.00 3000.00
Zinc (Zn) 43.75 38.15
Copper (Cu) 0.55 2.80
Iron (Fe) 78.80 175.40
Phosphorous (P) 120.12 0.14
Potassium (K) 0.25 4.65
Magnesium (Mg) 105 78
Manganese (Mn) 1.23 0.22
Silicon (Si) Nil 2.93

Table 2. Elemental composition of quail egg (Coturnix japonica).

Statistical analysis of the mean blood glucose levels at d 14 and 21 after treatment of diabetic induced rats with quail egg showed that quail eggs were able to effectively lower the blood glucose to almost the same level with the control (group 1) and diabetic insulin treated rat (group 3) (Table 3).

Group Before induction (mg/dl) D 1 induction (mg/dl) D 7 induction (mg/dl) D 14 induction (mg/dl) D 21 induction (mg/dl)
1 (NC) 81.25 ± 5.74 121.75 ± 1.31a 101.75 ± 4.55a 85.50 ± 3.57a 114.00 ± 4.42a
2 (DNT) 57.50 ± 10.46 255.75 ± 53.51b 325.50 ± 40.13b 381.25 ± 38.23b 417.50 ± 43.19b
3 (DTI) 58.75 ± 11.10 177.00 ± 28.23c 170.75 ± 21.85c 189.00 ± 19.48c 146.75 ± 20.76c
4 (2PE) 86.50 ± 3.80 320.75 ± 15.44bd 219.25 ± 29.44cb 186.75 ± 32.07c 135.50 ± 23.70c
5 (3PE) 82.00 ± 2.48 464.25 ± 57.12d 366.75 ± 33.39b 287.00 ± 26.80cb 189.50 ± 13.07c
6 (2PES) 105.00 ± 7.65 506.25 ± 34.34e 345.75 ± 23.90b 233.25 ± 29.40cb 113.00 ± 8.04a
7 (3PES) 97.25 ± 8.75 386.75 ± 61.45bd 360.50 ± 62.58b 294.50 ± 51.95cb 143.75 ± 15.02c
8 (2RE) 79.75 ± 9.63 216.75 ± 56.04b 268.00 ± 57.80cb 179.75 ± 25.44c 107.00 ± 3.79a
9 (3RE) 74.00 ± 11.45 374.75 ± 64.43bd 343.00 ± 81.54b 222.00 ± 22.55cb 124.25 ± 2.46ac

Table 3. Blood glucose in mg/dl of alloxan induced diabetes rats treated with insulin, processed and raw quail eggs.

Diabetes is associated with weight loss and the result of this study revealed that diabetic rats treated with two and three raw quail eggs respectively showed the best performance in terms body weight gain when compared with other quail egg treated rats (Table 4).

Group Before induction 1 w after induction 2 w after induction 3 w after induction
1 (NC) 150.98 ± 7.38 170.00 ± 7.18 194.35 ± 5.41 211.53 ± 6.55
2 (DNT) 193.58 ± 3.56 188.20 ± 4.42* 178.40 ± 3.48* 173.23 ± 4.59*
3 (DTI) 168.00 ± 8.49 181.95 ± 9.11 184.58 ± 4.95 187.58 ± 5.03
4 (2PE) 155.53 ± 5.44 155.50 ± 5.42* 164.15 ± 6.49 168.65 ± 6.03
5 (3PE) 187.28 ± 13.70 189.63 ± 11.29 192.88 ± 12.55 195.30 ± 10.37
6(2PES) 165.80 ± 11.55 174.38 ± 5.94 177.90 ± 6.63 181.95 ± 7.20
7(3PES) 189.90 ± 7.33 197.33 ± 12.51 191.53 ± 9.01* 192.15 ± 9.11
8 (2RE) 135.40 ± 9.93 133.40 ± 11.56* 140.25 ± 12.08 145.28 ± 15.08
9 (3RE) 198.40 ± 8.61 204.33 ± 9.24 213.23 ± 6.99 221.23 ± 5.01

Table 4. Weight (g) of alloxan induced diabetes rats treated with insulin, processed and raw quail eggs.

The lipid profile analysis revealed that quail egg treatment/ administration did not have any negative effect on the lipid profile level of diabetic rats but showed ability of reducing/ lowering the lipid profile, implying that quail egg diet could be a possible means of reducing the risk of heart disease or any risk of diabetic dyslipidaemia (Table 5).

Group Cholesterol (mg/dl) HDL (mg/dl) TG (mg/dl) VLDL (mg/dl) LDL (mg/dl)
1 (NC) 108.50 ± 4.79a 37.75 ± 6.87a 61.00 ± 7.38a 15.25 ± 1.31 38.00 ± 8.66a
2 (DNT) 135.00 ± 5.93b 59.00 ± 9.70b 81.25 ± 10.08b 16.25 ± 2.02 59.75 ± 5.89b
3 (DTI) 103.00 ± 8.57a 50.75 ± 6.87c  680.00 ± 5.28c 16.00 ± 1.08 36.25 ± 4.21c
4 (2PE) 100.25 ± 5.14a 38.50 ± 2.25 72.75 ± 10.73 14.50 ± 2.10 46.50 ± 2.40c
5 (3PE) 81.75 ± 9.82a 36.50 ± 5.14 71.00 ± 24.86 14.25 ± 5.02 23.75 ± 2.63
6 (2PES) 105.75 ± 14.21a 52.25 ± 7.43c 91.25 ± 16.64 18.25 ± 3.30 35.25 ± 7.55c
7 (3PES) 88.00 ± 5.18a 45.50 ± 3.59c 58.50 ± 10.12 12.00 ± 2.16 30.50 ± 6.22
8 (2RE) 70.75 ± 5.02a 29.25 ± 3.94 62.75 ± 10.66 12.50 ± 2.10 29.00 ± 0.71
9 (3RE) 89.00 ± 4.04a 44.25 ± 1.03 84.75 ± 9.08 17.00 ± 1.83 27.75 ± 3.25
**Normal Range: 86.19-144.76 50.00-56.67 47.57-108.11 9.51-21.62 11.24-77.46

Table 5. Lipid profile of all the groups after treatment with processed and raw quail egg, quail egg shell.

Discussion

Quail eggs are considered a delicacy in many parts of the world and have been used for medicinal purposes for hundreds of years. The results of the proximate composition revealed that quail eggs could be a good source of protein and lipids; though, with minimal ash and carbohydrate contents. Similar findings have been reported by Tolik et al., Dudusola and Genchev [20-22]. On the contrary, Sinanoglou et al. [23] reported lower fat and higher ash contents in quail egg yolk. This study (Table 2) showed that the whole egg was rich in calcium and magnesium, while the egg shell had higher content of calcium. The whole egg showed high content of magnesium and phosphorus compared to the egg shell; similar observation was made by Shanaway [24]. Abduljaleel et al. [25] in their study showed higher manganese, iron, zinc and copper content in quail egg than what was obtained in this study. The difference in mineral content could be as a result of the major constituent of feed used in feeding the birds or the different environment the birds were exposed to as avian eggs mineral compositions have been reported to vary as a result of region of origin [26].

On d 7 of the treatment, it was observed that insulin treated group showed the earliest sign of recovery unlike other groups which implied that the groups treated with the quail eggs did not have a short term effect on blood glucose level. On days 14 and 21, no statistical difference was observed between normal control group, diabetic rats treated with insulin and most of other groups treated with quail egg. At this point the quail egg had almost the same effect as insulin with group 8 (raw quail egg diet) showing the lowest blood glucose level (Table 3). This showed that the administration of quail egg for 21 d lowered the blood glucose level of diabetic rats to the level of the normal control group, which suggests the long term beneficial effect of quail egg in diabetes treatment. It was also indicated that quail egg treatments had the same effect as insulin and could be substituted for insulin in the long-term effect of diabetes management and treatment. The hypoglycaemic effect of quail egg may be attributed probably to the presence of minerals and amino acids which play key role in blood glucose homeostasis by regulating the key enzymes involved in glucose metabolism [27]. On the contrary, Lontchi-Yimagou et al. [28] reported that administration of quail eggs for 16 d in diabetic rats had no effect on blood glucose.

Diabetes mellitus is associated with weight loss. In this study, weight loss was recorded in d 7 and 14 in some alloxan induced diabetic groups and this resembles a common observation in clinical diabetes. On d 21, all the treated groups recorded weight gain which could be seen in normal rats (Table 4). This implies that the effect of weight loss associated with diabetes had been effectively addressed by administration of quail egg. This observation showed that quail egg could be effectively used for both mid-term and long-term treatment of weight loss associated with diabetes. The high protein content of quail egg could be attributed to the observed weight gain [29].

This study observed that administration of quail egg to alloxan induced diabetic albino rats did not have any negative effect on the lipid profile of diabetic rats but has the ability of lowering the lipid profile (Table 5) thereby reducing the risk of diabetic dyslipidaemia. This finding is in agreement with the study carried out by Aba et al. [30] which showed that alloxanized rats that were administered with quail egg solution demonstrated hypolipidemia. Lontchi-Yimagou et al. [28] in their study showed that total cholesterol and triglyceride levels were higher in diabetic rats receiving quail eggs compared to non-treated diabetic rats and diabetic untreated rats respectively. The finding here suggests that intake of quail egg diets either alone or as part of a therapeutic regime, could have beneficial effect in prevention and management of diabetes mellitus.

References