Effects of monocular enucleation on the lateral geniculate nucleus (LGN) of rabbit: A qualitative light and electron microscopic study
Department of Anatomy, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, India
- *Corresponding Author:
- Dr. Aijaz Ahmed Khan
Department of Anatomy, J.N. Medical College
Aligarh Muslim University Aligarh 202002, India
E-mail: aijazahmedkhan7 ( at ) hotmail.com
Accepted date: July 17 2004
The lateral geniculate nucleus (LGN) is the thalamic relay center in the visual pathway. Deafferentation induced by different methods is known to result into nerve fibre and nerve cell degeneration. However, information about the minimum time required for such changes to appear is rather scanty. In the present study 20 adult rabbits received monocular enucleation and the non-enucleates served as controls. The operated animals survived from 12h to 3m before they were sacrificed. The lateral geniculate nuclei from the perfusion fixed animals were processed for light and electron microscopic observations. It was found that 24h was the least time required for detectable morphological changes to appear. The light microscopic changes were in the form of swelling of nerve fibres, occurrence of minute holes followed by neuronal death. The spared neurons revealed hypertrophy and gliocytes showed hyperplasia. Electron microscopy revealed loosening of myelin lamellae, intramyelinic oedema, periaxonal vacuolation and electron-dense degeneration of the nerve fibres. With increasing duration these changes also progressed in number, size and morphology followed by neuronal death, glial cell hyperplasia and hypertrophy. Presynaptic axon terminals degenerated earlier than post-synaptic profiles. Conclusions: (i) Minimum time required for microscopic changes to appear was just 24h. (ii) Neurons heavily dependent on retinal inputs degenerated and the least dependent ones hypertrophied. (iii) All progressive degenerative changes could not be rigidly placed in temporal sequence.
Eye enucleation, deafferentation, lateral geniculate nucleus, transneuronal degeneration
The lateral geniculate nucleus (LGN) occupies a pivotal position in the visual pathway between retina and the visual cortex. It consists of six laminae in man and rhesus monkey, three in cat and poorly defined two laminae in rabbit. LGN receives afferent inputs from the retina, visual cortex and many other subcortical centers. The retinal inputs to LGN are predominantly from the contralateral retina. Ocular enucleation in experimental animals leads to transneuronal degeneration [1,2,3], and reduction in parvalbumin  calbindin [4,5], expression of calbindin and parvalbumin  as well as density of highly immunoreactive neurons . It is also associated with increase of substance-P immunoreactivity , gilal fibrillary acidic protein , 160 kd neurofilament . Deprivation of light for just 48h has been shown to cause apoptotic cell death . The morphological substrate for many of the above findings after short-term depri-vation and deafferentation are not clearly defined. The present study was undertaken to find out both early and late qualitative microscopic changes in the contralateral LGN of rabbit after ocular enucleation.
Materials and Methods
After approval of the ethical committee, 20 adult white rabbits of either sex received unilateral ocular enucleation. The nonenucleates served as controls. In the experimental groups the left side of the eye was removed under general anaesthesia by standard operative procedure known as ‘lateral canthotomy.’ Animals were sacrificed at postoperative survival periods of 12h, 1d, 1w, 1m, and 3m. Tissue samples were obtained after intracardiac perfusion of buffered formalin for light microscopy and Karnovsky fixative for electron microscopy. 8 μm thick paraffin sections stained with Haematoxylin-eosin, Cresyl violet and Luxol fast blue as well as 0.5 μm thick araldite sections stained with Toluidine blue were observed under light microscope (Olympus – BH2). Uranyl acetate and lead citrate stained 70 nm ultrathin araldite sections were used for electron microscopy (Philips CM12).
Gross. The contralateral LGN remained akin to ipsilateral and control LGN up to 2m. At 3m, it showed apparent shrinkage.
Samples at 12 h revealed no demonstrable change. After 24h, the changes were in the form of generalized swelling of nerve fibres covering the external surface of LGN, with occasional holes or minute cavities. In the samples from the subsequent periods these features became more frequent and prominent. At 3m postenucleation (Fig. 1) the myelin degeneration could be appreciated even at a very low magnification. At higher magnification, LGN in its superficial part showed pleomorphic cavities, myelin degeneration, fragmentation and ovoid formation. Multi-ple degenerating myelinated nerve fibres projected in the large cavities (Fig. 2A). The deeper part of the LGN consisted of geniculocortical neurons characterized by large euchromatic nucleus, prominent nucleolus, and large cell body containing Nissl substance. Samples from 1 month and onwards, showed vacuolation and cell death. Dead cell (Fig. 2B) was characterized by condensed, darkly stained nucleus with pale cytoplasm devoid of organelles. The saved neurons were either similar to controls or were hypertrophied (Fig. 2C). The gliocytes outnumbered neurons more prominently in experimental groups. These cells were characterized by small heterochromatic nucleus having darkly stained thin rim of cytoplasm.
It showed ever-growing periaxonal oedema and vacuolations. These vacuolations (Fig. 3A) began within the myelin sheath or between the myelin and the axon. The myelin split and there developed intramyelinic accumulation of amorphous and occasionally polymorphic electron dense material. The degenerating axon was pushed to an eccentric position. The neurons not affected by enucleation were comparable to controls (Fig. 3B) had large vesicular nucleus, prominent nucleolus and perikaryon was full of organelles. The glial cells were characterized by irregular heterochromatic nucleus and thin rim of cytoplasm (Fig. 3C) and were occasionally associated with degeneration debris. Some glial cells located in the vicinity of a dead cell became hypertrophied. The dead cell (Fig. 3C) was characterized by a shrunken, heterochromatic nucleus, clumps of chromatin and poorly defined nuclear membrane as well as perikaryon devoid of organelles. Often the dead cells were associated with degenerating terminals (Fig. 3C). The presynaptic terminals showed degenerative changes earlier than the postsynaptic terminals.
Figure 3: Fig. 3A EM of LGN at 1 week postenucleation showing intramyelinic oedema, splitting of myelin lamellae (↑); aggregation of dense and amorphous material and the axon (٭)
pushed to one side. X8600
Fig. 3B EM of LGN at 1m postenucleation, showing normal geniculocortical neuron characterised by large vesicular nucleus, prominent nucleolus, and perikarya full of organelles. X11000
Fig. 3C EM of LGN at 3m postenucleation showing a dead cell (٭) with shrunken heterochromatic nucleus, ill-defined nuclear membrane and perikarya devoid of organelles. Hypertrophied glial cell (G), and degenerating synaptic profiles (↑) adjacent to the dead cell. X1100
Experimental ocular enucleation isolated the retinal ganglion cells from their axons. This lead to anterograde degeneration of these axons constituting the optic nerve and optic tract right up to the lateral geniculate nucleus . Since the contralateral LGN, received major input , this showed the major post-lesional changes. Gross examination finding of reduction in the size of contralateral LGN at 3m was in agreement with the observation of 40% reduction in the contralateral retinorecepient region of superior colliculus in enucleated rats . The degenerative change was demonstrated just after 24h which was a bit earlier than 30h  and 48h  but comparable to those found in the ipsilateral optic nerve, and contralateral optic tract after enucleation . The vacuolations observed were due to oedema, breaking down of myelin while larger cavities in the advancing enucleates suggested complete dissolution of myelin. The deeper cell-rich region showed less number of vacuolations, and when present they were either due to complete dissolution of affected fibres or resorption of dead cells. The dead cells noticed in present study had light microscopic features comparable to apoptotic cells noticed in human LGN  during development. Demonstration of degeneration product up to 3m was possible because the process of degeneration and removal of degeneration debris is known to be slower in central nervous system as compared to peripheral nervous system . Con-comitant presence of both dead (denervated) and hypertrophied (spared) cells was in partial agreement with some [1,2] studies.
The observation that the myelin sheaths usually do not collapse but become dilated was in agreement with some , and in partial agreement with other , suggesting that the myelin sheaths collapse and lamellae become loose but the sheath may remain intact up to 2m. The ultrastructural features of dead cell observed were comparable to those of apoptotic cells noticed in developing human LGN . Presence of hypertrophied neurons in conjunction with the dead cells suggested hypertrophied cell’s involvement in the formation of new synaptic contacts. However, the spared hypertrophied cells were not associated with free postsynaptic densities as noticed during normal course of synaptogenesis during foetal life  as well as during ‘de novo’ synapse formation after decortication-induced deafferentation in in adults [22,23]. This probably reflected limited morphogentic potentiality of adult brain to develop new contacts. The features of degenerating terminals in terms of loss of definition of organelles, aggregation of dense bodies, degeneration of mitochondria and collection of neurofilaments matched with some  studies. The glial cells and pericytes located in close vicinity of the dengenerating cells and terminals in this study suggested that astrocytes and microglial cells take part in removal of degeneration debris [25,26]. Some workers  are of the view that even pericytes have the properties of macrophages and have potential to actually develop and prolif-erate into macrophages. It was concluded that: (i) While morphological changes may be detected as early as 24h after lesion, the degeneration products remained for months together. (ii) Enucleation induces death of certain LGN neurons and compensatory hypertrophy of others. (iii) Various degenerative changes observed could not be rigidly put in a rigid temporal sequence.
I gratefully acknowledge the financial support, laboratory facilities and technical assistance provided by the University Science Malaysia. Malaysia.
- Sloper JJ, Headon MP, Powell TP. Effects of enucleation at different ages on the sizes of neurons in the lateral genicu-late nucleus of infant and adult monkeys. Brain Res 1987a; 428: 259-265.
- Sloper JJ, Headon MP, Powell TP. Changes in the size of cells in the monocular segment of the primate lateral geniculate nucleus during normal development and following visual deprivation. Brain Res 1987b; 428: 467-476.
- Tigges M, Tigges J. Parvalbumin immunoreactivity of the lateral geniculate nucleus in adult rhesus monkeys after monocular eye enucleation. Vis Neurosci 199; 6: 375-382.
- Mize RR, Luo Q. Visual deprivation fails to reduce calbindin 28kD or GABA immunoreactivity in rhesus monkey superior colliculus. Vis Neurosci 1992; 9: 157-168.
- Smith SA, Bedi KD. Unilateral eye enucleation in adult rats causes neuronal loss in the contralateral superior colliculus. J Anat 1997; 190: 481-490.
- Gutierrez C, Cusick CG. Effects of chronic monocular enucleation on calcium binding proteins calbindin-D28k and parvalbumin in the lateral geniculate nucleus of adult rhesus monkeys. Brain Res 1994; 651: 300-310.
- Blumcke I, Weruaga E, Kasas S, Hendrickson AE, Celio MR. Discrete reduction patterns of parvalbumin and calbindin D-28k immunoreactivity in the dorsal lateral geniculate nucleus and the striate cortex of adult macaque monkeys after monocular enucleation. Switzerland Vis Neurosci 1994; 11: 1-11.
- Luo XG, Kong XY, Wong-Riley MT. Effects of monocular enucleation or impulse blockage on gamma-aminobutyric acid and cytochrome oxidase levels in neurons of the adult cat lateral geniculate nucleus. Vis Neurosci 1991; 6: 55-68.
- Miguel-Hidalgo JJ, Senba E, Takatsuji K, Tohyama M. Effects of eye enucleation on substance P- immunoreactive fibres of some retinorecipient nuclei of the rat in relation to their origin from the superior colliculus. Neurosc 1991; 44: 235-243.
- Candy KS, Olavarria JF, Rubel EW. Reduced retinal activity increases GFAP immunoreactivity in rat lateral geniculate nucleus. Brain Res 1994; 663: 206-214.
- Capani F, Loidl CF, Pecci SJ. Unilateral enucleation induces an increase of 160 kd neurofilament in lateral geniculate nuclei synapses. Biocell 1996; 20: 55-59.
- Nucci C, Piccirilli S, Palma E, Bagetta G, Nistico G, Ceulli L. N omega-nitro-L- arginine methyl ester prevents apoptosis induced in the lateral geniculate nucleus by light deprivation in adult rabbit. Neuroreport 1996; 7: 2863-2866.
- Kupfer C. Chumbley L , Downer J de C. Quantitative histology of optic nerve, optic tract and lateral geniculate nucleus of man. J Anat 1967; 101: 393-401.
- iolli RA, Guthrie MD. The primary optic projection in rabbit. An experimental degeneration study. J Comp Neurol 1969; 136: 99-126.
- Smith SA, Bedi KD. Unilateral eye enucleation in adult rat causes neuronal loss in the contralateral superior colliculus. J Anat 1977; 190: 481-490.
- Cajal SR. (1928) Degeneration and Regeneration of the Nervous System (translated by May RM), 1928; Oxford University Press, London.
- Daniel PM, Strich SJ. (1969) Histological observations on the Wallerian degeneration in the spinal cord of the baboon, Papio papio. Acta Pathologica 1969; 12: 314-328.
- Khan AA. Wallerian degeneration in the optic nerve of rabbit. Cells Tissues Organs 2004: 177: 104-109.
- Khan AA, Wadhwa S, Bijlani V. Prenatal human lateral geniculate nucleus: A quantitative lightmicroscopic study. Dev. Neurosci 1993; 15: 403-409.
- Daniel PM, Strich SJ. Histological observations on the Wallerian degeneration in the spinal cord of the baboon, Papio papio. Acta Pathologica 1969; 12:314-328.
- Duchen LW. General pathology of neurons and neuroglia. In Greenfield’s neuropathology, 5th Ed. By Adams JH and Duchen LW. 1992: Pp 1-68.
- Khan AA, Wadhwa S, Bijlani V. Development of human lateral geniculate nucleus: An electronmicroscopic study. Int J Dev Neurosci 1994; 12: 661- 672.
- Hamori J, Silakov VL. Plasticity of relay neurons in the dorsal lateral geniculate nucleus of the adult cat: morphological evi-dence. Neurosci 1980; 5: 2073-2077.
- Hamori J. "De novo" formation of synapses by experimentally induced presynaptic dendrites in adult mammalian brain. Acta Biol Acad Sci hun 1982; 33 (2-3): 173-187.
- Vaughn JE , Pease DC. Electron microscopic studies of Wallerian degeneration in rat optic nerve II. Astrocytes, oligodendrocytes and adventitial cells. J Comp Neurol 1970; 140: 207-226.
- Walberg F. An electron microscopic study of terminal degeneration in the inferior olive of the cat. J Comp Neurol 1965; 125: 205-225.
- Vaughn JE, Hinds PL, Skoff RP. Electron microscopic studies of Wallerian degeneration in the optic nerve. I. The multipotential glia. J Comp Neurol 1970; 140: 175-206.