Effect of EGFR gene mutation in patients with TNBC treated with bevacizumab combined with gemcitabine
Department of Mammary Gland Surgery, Zhongshan Hospital, Sun Yat-sen University, Guangzhou, PR China
- *Corresponding Author:
- Shihui Ma
Department of Mammary Gland Surgery, Zhongshan Hospital
Sun Yat-sen University, PR China
Accepted date: April 4, 2017
Objective: To investigate the effect of Epidermal Growth Factor Receptor (EGFR) gene mutation in patients with Triple Negative Breast Cancer (TNBC) treated with bevacizumab combined with gemcitabine.
Methods: 180 patients with triple-negative breast cancer admitted to our hospital from September 2011 to September 2013 were enrolled in this study. According to their DNA sequencing results, the patients were divided into EGFR mutation group and the non-mutation group, and according to the patient's genetic mutation type, patients in mutation group were divided into exon 19 deletion group, exon 21 mutation group. All patients were treated with bevacizumab and gemcitabine, and the prognosis of each group was compared.
Results: EGFR mutations were found in 51 cases (28.3%). The sex ratio and smoking history of EGFR mutant group were significantly different (P<0.01) compared with non-mutated group. For EGFR mutation group, there were 30 cases (58.8%) of exon 19 deletion, and 21 cases of exon 21 mutation (41.2%). The progression-free survival time and 1-year survival rate were significantly higher in mutant group than in non-mutation group (P<0.05). The total effective rate in EGFR exon 19 deletion group, exon 21 mutation group was 80.0% and 76.2%, higher than 40.3% in the non-mutation group, the disease control rate was also higher than the mutation group, the difference was statistically significant (P<0.05). There was no significant difference in total effective rate and disease control rate between exon 19 deletion mutation group and exon 21 mutation group (P>0.05). Adverse reactions were concentrated in the rash, diarrhea, with mild degree and tolerable. For the incidence of adverse reactions in three groups, the difference was not statistically significant (P>0.05).
Conclusion: EGFR mutations are more sensitive in patients with triple-negative breast cancer treated with bevacizumab and gemcitabine, with better clinical efficacy, and the progression-free survival of tumors is prolonged. EGFR mutation has no significant effect on the safety of treatment.
Epidermal growth factor receptor, Gene mutation, Bevacizumab, Gemcitabine, Triple negative breast cancer.
Three negative breast cancer is a common subtype of breast cancer, with aggressive, high recurrence rate, distant metastases and other characteristics. The prognosis of patients is often in poor quality . The combination of bevacizumab and gemcitabine in the treatment of triple-negative breast cancer after chemotherapy failed in the second and third line treatment has been widely concerned about, the safety and tolerance of which are better for patients with advanced triplenegative breast cancer. It also has certain significance in improving the quality of life . In recent years, some scholars pointed out that Epidermal Growth Factor Receptor (EGFR) and tumor angiogenesis, invasion and metastasis are closely related, and EGFR gene mutations have some impact in patients with chemotherapy . To determine the EGFR gene mutation in triple negative breast cancer in the treatment of bevacizumab combined with gemcitabine, 180 cases of triplenegative breast cancer patients in our hospital from September 2011 to September 2013 were prospectively studied, intending to evaluate the differences between efficacy and adverse reactions, the research process and conclusions are reported as follows.
Case information: One hundred and eighty patients with triple-negative breast cancer admitted to our hospital from September 2011 to September 2013 were included in the study after informed consent obtained and approved by the Medical Ethics Transfer Committee. The mean age of patients was 32~75 years old (54.20 ± 19.17) years old. The ECOG functional status score was 0~1, with mean of (0.71 ± 0.20). Menstrual state: 74 cases of menopause, 96 cases of no menopause. Metastatic sites: 57 cases of liver metastasis, lung metastasis in 26 cases, 17 cases of other parts of the metastasis.
Inclusion criteria and exclusion criteria: Inclusion criteria: (1) the first diagnosis of triple negative breast cancer using pathological histology [4,5]. (2) Histological stage of IIIa~IV, with no radical surgery indications. (3) Measurable lesions ≥ 1. (4) The expected survival ≥ 3 months. (5) No previous history of chemotherapy or chemotherapy failure. Exclusion criteria: (1) Refusal to participate in the study, or drug compliance is poor. (2) The complicated with heart, liver, kidney and other vital organs lesions or tumors.
180 patients were treated with bevacizumab and gemcitabine. Gemcitabine (trade name: Yu Jie, Guoyaozhunzi H20063675, Harbin Yu Heng Pharmaceutical Co., Ltd., specifications of 1.0 g) was administered by intravenous infusion, each time for 1.0 g/m2 at day 1 and day 8. Bevacizumab (Trade name: Avastin, Registration No. S20100023, Roche Pharmaceutical Company, Switzerland, specification 100 mg: 4 mL) was given at days 2-4, each time for 60~70 mg/m2. 21 day for a treatment cycle, continuous treatment of 6 months, or with the end of treatment for tumor progression or unable to tolerate toxic and side effects .
The EGFR gene was detected by digestion-DNA extraction and purification-PCR amplification-sequencing . The sequencing equipment was ABI 3730XL automated DNA sequencer (US Applied Biosystems). According to their DNA sequencing results, patients were divided into EGFR mutation group and the non-mutation group, and according to the patient's genetic mutation type, they were divided into exon 19 deletion group and exon 21 mutation group.
The patients were followed up for 18 months by telephone and outpatient follow-up. Their progression-free survival time, adverse reaction status and tumor progression-free survival time were recorded at the end of follow-up in March 2015. Tumor progression-free survival: treatment initiation to tumor progression or death time. Survival: time of treatment start to death.
Clinical efficacy was evaluated at the time of last follow-up with reference to the Response Evaluation Criteria in Solid Tumors (RECIST) . Complete remission (CR): the lesion completely disappeared; 2. Partial remission (PR): the sum of the longest diameter of lesions decreased ≥ 30% than before the treatment; 3. Stable Disease (SD): the sum of the longest diameter of lesions decreased<30% than before the treatment; 4. Progressive Disease (PD): the sum of the longest diameter of lesions increased ≥ 20% than before the treatment, or new lesions appeared. Total effective rate=(CR+PR)/total number of cases × 100%, disease control rate=(CR+PR+SD)/total number of cases × 100%.
All data for this clinical study were analysed using SPSS 18.0, count data expressed in (n/%), and with the use of χ2 test. Measurement data were represented as (͞x ± s), and tested with t test. Test level was set to α=0.05. P<0.05 was considered statistically significant, with P<0.01 was statistically extremely significant.
EGFR gene mutations in patients
EGFR mutations were found in 51 patients (28.3%). The sex ratio and menstrual status of EGFR mutant group were significantly different (P<0.01) compared with non-mutation group (Table 1). In EGFR mutation patients, there were 30 cases of exon 19 deletion (58.8%), and 21 cases of exon 21 mutation (41.2%).
|Indexes||Mutation group(n=51)||Non-mutation group(n=129)||t/χ2||P|
|Age (years)||53.95 ± 18.42||55.10 ± 18.85||0.139||>0.05|
|Functional status score (points)||0.70 ± 0.13||0.73 ± 0.15||0.047||>0.05|
|Menopausal status||Menopause||35 (68.6)||39 (30.2)||19.365||<0.01|
|No menopause||16 (31.4)||80 (62.0)|
|Metastatic sites||Local lymph nodes||38 (74.5)||107 (82.9)||0.747||>0.05|
|Liver||16 (31.4)||41 (31.8)||0.085||>0.05|
|Lung||9 (17.6)||17 (13.2)||0.224||>0.05|
|Other parts||5 (9.80)||12 (9.30)||0.139||>0.05|
Table 1: Clinical data comparison between EGFR gene mutations and non-mutations (n/%).
EGFR mutation in survival of patients
The tumor progression-free survival time and 1-year survival rate were significantly higher in mutant group than in nonmutation group (P<0.05, Table 2).
|Indexes||Mutation group (n=51)||Non-mutation group (n=129)||t/χ2||P|
|Tumor progression-free survival time (Months)||11.96±2.84||7.21±3.90||4.663||<0.05|
|1 year survival rate (n/%)||40(78.4)||73(56.6)||9.721||<0.05|
Table 2: Survival time comparison between EGFR gene mutations and non-mutations (͞x ± s).
EGFR mutation in the clinical efficacy of patients
The total effective rate in EGFR exon 19 deletion group, exon 21 mutation group was 80.0% and 76.2%, higher than 40.3% in the non-mutation group, the disease control rate was also higher than the mutation group, the difference was statistically significant (P<0.05). There was no significant difference in total effective rate and disease control rate between exon 19 deletion mutation group and exon 21 mutation group (P>0.05, Table 3).
|Clinical efficacy||Mutation group (n=51)||Non-mutation group (n=129)|
|EGFR exon 19 deletion group (n=30)||EGFR exon 21 mutation group (n=21)|
|CR||3 (10.0)*||2 (9.5)*||16 (12.4)|
|PR||21 (70.0)*||14 (66.7)*||36 (27.9)|
|SD||5 (16.7)||4 (19.0)||25 (19.4)|
|PD||1 (3.3)*||1 (4.8)||52 (40.3)|
|Total effective||24 (80.0)*||16 (76.2)*||52 (40.3)|
|Disease control||29 (96.7)*||20 (95.2)*||77 (59.7)|
Table 3: Comparison of clinical efficacy among the groups (n/%).
EGFR mutations in patients with adverse effects
Adverse reactions were concentrated in the rash, diarrhea, with mild degree and tolerable. For the incidence of adverse reactions in three groups, the difference was not statistically significant (P>0.05, Table 4).
|Adverse reactions||EGFR exon 19 deletion group (n=30)||EGFR exon 21 mutation group (n=21)||Non-mutation group (n=129)|
|Rash||17 (56.7)||11 (52.4)||68 (52.7)|
|Diarrhea||7 (23.3)||5 (23.8)||31 (24.0)|
|Xerosis cutis||4 (13.3)||3 (14.3)||18 (14.0)|
|Anorexia||4 (13.3)||2 (9.5)||13 (10.1)|
|Others||8 (26.7)||6 (28.6)||37 (28.7)|
Table 4: Comparison of adverse reactions among the groups (n/%).
Breast cancer is the first and second killer in female malignant tumors by incidence and mortality, of which three negative breast cancer is a subtype of breast cancer to negative estrogen receptor, progesterone receptor and human epidermal growth factor 2 as the main feature, of such patients with poorly differentiated cancer cells and high invasion [9-11]. At the same time, because of hormone receptor expression were negative, sensitivity of triple negative breast cancer to endocrine therapy is poor, with the lack of effective treatment. In recent years, molecular targeted technology has been a focus of attention in triple-negative breast cancer treatment. These drugs target at the tumor cell line of specifically, and with less damage to normal cells. They bring a new hope for treatment of patients with triple-negative breast cancer of poor general situation and low chemotherapy tolerance.
Bevacizumab is a humanized monoclonal antibody directed against Vascular Endothelial Growth Factor (VEGF), which is composed of 7% murine structure and 93% human Immunoglobulin G (IgG). It can be combined with VEGF receptor by competitive binding, so as to inhibit VEGF biological activity, thus blocking VEGF playing the role in endothelial cell mitosis, neovascularization, finally inhibiting the growth of tumor cells to achieve the purpose. At the same time, bevacizumab has effect on reducing vascular permeability and interstitial pressure. It not only can promote the normalization of tumor blood vessels, but also enhance the concentration of other chemotherapy drugs [12-14]. Bevacizumab has been widely used in the first-line treatment of metastatic colon cancer and breast cancer since the beginning of the 21st century.
Gemcitabine is a nucleoside antimetabolite whose metabolites have unique antitumor activity by interfering with DNA strand synthesis and depletion of deoxynucleotides, inhibiting DNA synthesis, leading to gemcitabine-induced apoptosis. A large number of studies have shown that the mechanism of gemcitabine had no cross-resistance with anthracycline or taxane, so it can still achieve high efficacy after the treatment failure of anthracycline or taxane in second or third line chemotherapy [15,16].
In recent years, a number of clinical studies found EGFR gene mutation in triple-negative breast cancer patients, and that this mutation had an impact on the treatment effect of molecular targeted drug [17,18]. On this basis, we selected 180 cases of triple-negative breast cancer patients for clinical study and found that EGFR mutation rate was 28.3%, and exon 19 deletion, exon 21 mutation based, consistent with the conclusions of the study of Lim et al. . The patients with EGFR mutations had a significantly better progression-free survival time and clinical efficacy than those of the nonmutation group after treatment with bevacizumab plus gemcitabine. The safety and tolerability were excellent, suggesting that bevacizumab combined with gemcitabine have a better effect on treatment of EGFR mutations in triplenegative breast cancer patients, significantly higher than the study of Prat et al.  who used gemcitabine only. The reason may be that EGFR mutation causes EGFR overexpression, activates Ras/Raf/MEK/ERK-MAPK pathway, enhances the sensitivity of micrometastasis, remnant tumor and sensibility of tumor cells to chemotherapeutic drugs, and increases the benefit of chemotherapy.
In summary, there is triple-negative breast cancer patients with EGFR mutations, who can achieve a more significant benefit after receiving bevacizumab combined with gemcitabine treatment. The tumor progression-free survival time and clinical efficacy were better than that in non-mutated patients. Patients with the program had a good tolerance. In future clinical treatment, patients with EGFR gene mutation should be detected first, the implementation of reasonable treatment programs should be implemented to improve the quality of their prognosis and prolong their survival. The gemcitabine combined bevacizumab chemotherapy on prolonged survival rate of patients should also be studied.
- Ilieva KM, Marlow R, Cheung A. A translational platform to design antibodies targeting triple negative breast cancer-specific antigens for cancer immunotherapy. Cancer Res 2015; 75: 1324-1324.
- Foulkes WD, Smith IE, Reis-Filho JS. Triple-negative breast cancer. N Engl J Med 2010; 363: 1938-1948.
- Nakajima H, Ishikawa Y, Furuya M, Sano T, Ohno Y. Protein expression, gene amplification, and mutational analysis of EGFR in triple-negative breast cancer. Breast Cancer 2014; 21: 66-74.
- Lehmann BD, Pietenpol JA. Identification and use of biomarkers in treatment strategies for triple-negative breast cancer subtypes. J Pathol 2014; 232: 142-150.
- Liedtke C, Mazouni C, Hess KR. Response to neoadjuvant therapy and long-term survival in patients with triple-negative breast cancer. J Clin Oncol 2008; 26: 1275-1281.
- Sahlberg KK, Bottai G, Naume B. A serum microRNA signature predicts tumor relapse and survival in triple-negative breast cancer patients. Clin Cancer Res 2015; 21: 1207-1214.
- Baselga J, Gomez P, Greil R. Randomized phase II study of the anti–epidermal growth factor receptor monoclonal antibody cetuximab with cisplatin versus cisplatin alone in patients with metastatic triple-negative breast cancer. J Clin Oncol 2013; 31: 2586-2592.
- Gelmon KA, Tischkowitz M, Mackay H. Olaparib in patients with recurrent high-grade serous or poorly differentiated ovarian carcinoma or triple-negative breast cancer: a phase 2, multicentre, open-label, non-randomised study. Lancet Oncol 2011; 12: 852-861.
- Park HS, Jang MH, Kim EJ, Kim HJ, Lee HJ. High EGFR gene copy number predicts poor outcome in triple-negative breast cancer. Mod Pathol 2014; 27: 1212-1222.
- Dawood S. Triple-negative breast cancer: epidemiology and management options. Drugs 2010; 70: 2247-2258.
- Cleator S, Heller W, Coombes RC. Triple-negative breast cancer: therapeutic options. Lancet Oncol 2007; 8: 235-244.
- Noguera-Troise I, Daly C, Papadopoulos NJ, Coetzee S, Boland P. Blockade of Dll4 inhibits tumour growth by promoting non-productive angiogenesis. Nature 2006; 444: 1032-1037.
- Shaked Y, Henke E, Roodhart JML. Rapid chemotherapy-induced acute endothelial progenitor cell mobilization: implications for antiangiogenic drugs as chemosensitizing agents. Cancer Cell 2008; 14: 263-273.
- Ferraro DA, Gaborit N, Maron R. Inhibition of triple-negative breast cancer models by combinations of antibodies to EGFR. Proc Nat Acad Sci 2013; 110: 1815-1820.
- Huang P, Plunkett W. Induction of apoptosis by gemcitabine. Semin Oncol 1995; 22: 19-25.
- Plunkett W, Huang P, Xu YZ, Heinemann V, Grunewald R. Gemcitabine: metabolism, mechanisms of action, and self-potentiation. Semin Oncol 1995; 22: 3-10.
- Silver DP, Richardson AL, Eklund AC, Wang ZC, Szallasi Z. Efficacy of neoadjuvant Cisplatin in triple-negative breast cancer. J Clin Oncol 2010; 28: 1145-1153.
- Shah SP, Roth A, Goya R. The clonal and mutational evolution spectrum of primary triple-negative breast cancers. Nature 2012; 486: 395-399.
- Lim SO, Li CW, Xia W. EGFR signaling enhances aerobic glycolysis in triple-negative breast cancer cells to promote tumor growth and immune escape. Cancer Res 2016; 76: 1284-1296.
- Prat A, Adamo B, Cheang MC, Anders CK, Carey LA. Molecular characterization of basal-like and non-basal-like triple-negative breast cancer. Oncologist 2013; 18: 123-133.