- Biomedical Research (2012) Volume 23, Issue 1

## Determination of Sex from the Shaft and Tuberosity of Radius- A Multivariate Discriminant Function Analysis

**J.E. Waghmare ^{1*}, P.R. Deshmukh^{2}, P.J. Waghmare^{3}**

^{1}Department of Anatomy, MGIMS, Sevagram, India.

^{2}Department of Community Medicine, MGIMS, Sevagram, India.

^{3}Department of Biochemistry, MGIMS, Sevagram, India.

- Corresponding Author:
- J.E. Waghmare

Department of Anatomy Mahatma Gandhi Institute of Medical sciences Sevagram, Wardha (Maharashtra) 442102 India

**Accepted date:** September 21 2011

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### Abstract

198 adult human radii of known age, sex and race available in the bone bank of Department of Anatomy, Government Medical College Aurangabad (Maharastra) were used for the present study. Out of 198 bones 133 were of male and 65 were of female. Six measurements of radius were pushed into step-wise multivariate discriminant analysis. Among the six variables, the best function was obtained by antero-posterior diameter at mid-shaft (Ams) with the lowest Wilk’s lamda (0.503),the highest enigvalue (0.987), the highest canonical correlation (0.705), and the highest percentage of correct classification (90.4%).The second best function was obtained by Circumference at mid-shaft (Cms). Vertical diameter of the radial tuberosity (Vrt) shows far less classification capacity, because it includes the highest wilk’s lamda (0.830), the lowest enigvalues (0.205), the lowest canonical correlation (0.315), and the lowest percentage of correct classification (71.7%). Linear function was obtained using antero- posterior diameter at mid-shaft, circumference at mid-shaft and vertical diameter of radial tuberosity, witch could classify 89.9% of radii correctly.

## Keywords

Radius, bone, Discriminant analysis, sex determination.

## Introduction

Determination of the sex of the unknown decomposed body is one of the most important tasks in the medicolegal cases and skeletal remains play a very important role for establishing the identity of the subject [1]. Apart from forensic sciences, determination of sex is an equally important objective in the field of the physical anthropology, bioarchaeology, palaeodemography and anatomy. Various precedent studies have dealt with sexual dimorphism of pelvis, crania and long bones from the discriminant function analysis, accuracy of sex classification is 95% for adult pelvis and 92% for the skull [1,2]. Sexual dimorphism affects the dimensions of the bone, which differs within and between the populations [3]. These differences allow the development of discriminant functions which reduce the subjectivity of sexual diagnosis from morphologic traits [3]. The discriminant functions are population specific and differs with race and geographical location [3,4]. Skull, pelvis and sacrum are the bones of choice to determine the sex of an unknown individual. Sometimes in the medicolegal cases challenging situations comes when a doctor is asked to detect the sex of the decomposed body where these bones might not be available. In such cases, it is a big task to determine the sex of an individual in the absence of the above mentioned bones. Therefore, it is necessary to formulate discriminate functions for the other bones, especially the long bones like radius that is frequently found in skeletal remains. Here we present the utility of radius in determining the sex using its different measurements.

*Methodology*

Study samples: 198 adult human radii of known age, sex and race available in the bone bank of Department of Anatomy, Government Medical College Aurangabad (Maharastra) were used for the present study. Out of 198 bones 133 were of male and 65 were of female. All the bones were dry, free of damage or deformity and were fully ossified.

Measurements: Following measurements were taken of radius in millimeters as described below.

1. Circumference at mid shaft (Cms): A mid shaft point was marked with the help of the osteometer and then the circumference was measured at that point with the help of non-elastic thread.

2. Antero-Posterior diameter at mid-shaft ( Ams ): Holding the Radius bone in anatomical position the A-P diameter was measured at mid-shaft point.

3. Transverse diameter at mid shaft ( Tms ): Holding the Radius bone in anatomical position the transverse diameter is measured at mid-shaft point.

4. Circumference at radial tuberosity (Crt ): A point was marked at the maximum convexity of the radial tuberosity and circumference was measured.

5. Antero-Posterior diameter of the radial tuberosity ( Art): Holding the radius bone in anatomical position, at the point of maximum convexity of the radial tuberosity the A-P diameter was measured with the help of varnier caliper.

6. Vertical diameter of radial tuberosity (Vrt ): Vertical diameter of radial tuberosity was measured between transverce tangents at the uppermost and lowermost margins of radial tuberosity.

*Statistical analysis*

A t-test was applied to compare the mean values of the measurements among the two sexes. t-value shows the relative importance of sex difference between the two sexes. A step-wise multivariate discriminant analysis was carried out to produce a linear function to compare the measurements of radius bone in a way to best separate the two sexes using SPSS 12.0. One sample Kolmogorov- Smirnov test was applied to all the six measurements in each sex to test for normality assumption. None of the measurement deviated from the assumption of normal distribution. Box’s M was calculated and found that the covariance matrices for males and females were not significantly different (Box’s M= 0.088, p= 0.767) Log determinant were 3.11 and 2.96 respectively. Multi-collinearity was not statistically significant. The Wilk’s lamda and correctly classified percentages were calculated. Wilk’s lambda is the ratio of within group sum of squares to total sum of squares. Value close to zero implies high predictability of membership. A statistical significance level of 0.05 was used.

## Results

We studied 198 bones of which 133 (67.2%) were of
male while rest 65 (32.8%) were female bones. **Table 1**
shows mean values of different measurements of the
bones. All the measurements were significantly larger
among males than the females (p<0.001). Highest relative
significance was shown by antero-posterior diameter
at mid-shaft (t= 12.5).

**Table 2** shows discriminant functions using all the different
measurements individually. Antero-posterior diameter
at mid-shaft could classify 90.4% of bones

All the six measurements were pushed into step-wise multivariate discriminant analysis. Amongst the six measurements, three measurements namely; circumference at mid shaft, vertical diameter of radial tuberosity and antero-posterior diameter at mid-shaft contributed significantly to the discriminant function. The linear function best classifying males and females was as follows:

D = -14.9 + 0.12 (circumference at mid shaft) + 0.095 (vertical diameter of radial tuberosity) + 0.735 (anteroposterior diameter at mid-shaft)

Where D is the discriminant score. The group centroids
for males and females were 0.751 and -1.557 respectively
with cutting point (-0.00011). This could correctly classify
89.9% of the bones with eginevalue of 1.166 and canonical
correlation of 0.734. Sex wise plot of discriminant
scores is shown in **figure 1**. The equation had least wilk’s
lamda indicating best fit.

## Discussion

Krogman WM (1986) did the pioneer work in osteometrics to distinguish the sex differences in bones and with eginevale of 0.987 and canonical correlation of 0.705. It was followed by circumference at mid-shaft, which could correctly classify 84.3% of the bones with eginevalue of 0.791 and canonical correlation of 0.665.

showed the validity of the skeletal remains for detection of sex [1]. The former studies were generally on the pelvic and cranial elements as these bones have considerable variance in males and females. This variance is largely applied in the anthropometry, bioarcheology and forensic sciences for determination of sex. The pelvic and cranial bones get decomposed earlier than that of the long bones, so we can not depend on them [5]. Recently it has been proven that the long bone circumferences can determine the sex of skeletal remains in the absence of pelvis and cranium [3]. The long bones have an added advantage that their measurements can be easily defined, epiphyseal and diaphyseal diameters of these bones show significant difference in the males and females [3,4,6]. Even in the fragmentary long bones there are certain landmarks which have proven their efficacy for analysis of sex, such as circumference at nutrient foramen of tibia [7,8], mid-shaft circumference of femur [9], distal end of fibula [10], circumference of humeral head [3], circumference at radial tuberosity [3,4] etc. Mall [11]) has worked on radius using its length, head diameter and distal radial width with these parameters he could identify the sex in 90% of the radii correctly [11,12,13].

We have measured six parameters in the present study, these are circumference at mid shaft (Cms), circumference at radial tuberosity (Crt ), antero-posterior diameter of the radial tuberosity ( Art), vertical diameter of radial tuberosity (Vrt ), antero-posterior diameter at mid-shaft ( Ams ) and transverse diameter at mid shaft ( Tms ). All the parameters had significantly higher values in males than females. Out of all the six parameters, anteroposterior diameter at mid-shaft had the highest discriminative power classifying 90.4% of bones correctly with lowest wilk’s lamba (0.503), highest eginevalue (0.987) and canonical correlation (0.705). Second parameter showing higher discriminative power was circumference at mid shaft classifying 84.3% of the bones correctly with wilk’s lamda (0.558). Kazahiro sakaue (2004) could classify 91.0% of the radii correctly using the mid shaft of radius to determine the sex in Japanese population [6]. Our finding is in concordance with his findings. Circumference at radial tuberosity was the next best parameter (t= 10.27) which has classified 79.8% of the bones with wilk’s lamda (0.650). Safont (2000) and Tomohito (2009) also had used the Circumference at radial tuberosity for sex determimation in Japanese population and could correctly identify the sex in 92.8% and 81.0% of bones respectively. Out of all the six parameters one parameter i.e. Vertical diameter of the radial tuberosity, has shown very low discriminative power [Wilk’s lambda (0.830), enigevalue (0.205)] using this parameter alone we could classify only 71.7% of the bones correctly. Circumference at mid shaft, vertical diameter of radial tuberosity and antero- posterior diameter at mid-shaft has contributed significantly to the discriminant function by sex.

The linear function best classifying males and females was as follows:

D = -14.9 + 0.12 (circumference at mid shaft) + 0.095 (vertical diameter of radial tuberosity) + 0.735(anteroposterior diameter at mid-shaft)

Where D is the discriminant score. With this linear function we could determine sex in 89. 9% radii correctly. This indicates that a set of variables ( Cms, Vrt and Ams) tends to be better for sex diagnosis than single variable. Former studies have also shown that with combination of variables, the long bones have as strong a discriminating power as do the pelvis or cranium have [1,3,4].

From above discussion we can conclude that by doing the discrininant function analysis the sex of an unknown individual can be detected in case of non availability of the skull, pelvis or sacrum which are the bones of choice for sex determination. This could be of immense help to detect the sex of an unknown subject in the medico-legal cases, which is an important task for forensic science, anthropology and anatomy.

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