Research Article - Biomedical Research (2017) Volume 28, Issue 2

Comparison of synthetic mesh types in terms of adherence of Escherichia coli and development of mesh infections in a rat model

¹Department of General Surgery, Faculty of Medicine, Kocaeli University, Kocaeli, Turkey

²Department of Clinical Microbiology, Faculty of Medicine, Kocaeli University, Kocaeli, Turkey

³Department of General Surgery, Kocaeli Derince Training and Research Hospital, Kocaeli, Turkey

⁴Department of General Surgery, Abant Izzet Baysal University Medical School, Bolu, Turkey

⁵Department of General Surgery, Faculty of Medicine, Dumlupinar University, Kutahya, Turkey

⁶Depertment of Pathology, Faculty of Medicine, Kocaeli University, Kocaeli, Turkey

*Corresponding Author:

Ertugrul Kargi
Department of General Surgery
Abant Izzet Baysal University Medical School, Turkey

Accepted on June 9, 2016

Abstract

Aim: To compare different types of synthetic mesh in terms of adherence of microorganisms and induction of inflammation, and to determine the mesh type associated with the lowest level of infection in an infected environment.

Materials and Methods: Sixty Sprague-Dawley rats were randomly divided into six groups: I: Prolen Mesh (Ethicon) (polypropylene); II: HI-TEX PARP MP (polyethylene tereoxphthalate [PET]+polyether urethane (PEU)); III: Vypro 11 (Ethicon) (polypropylene+polygalactin 910); IV: Motif Mesh (Proxy Biomedical) (expanded polytetrafluoroethylene [ePTFE]; a composite mesh); V: Proceed Mesh (Ethicon) (absorbable oxidised regenerated cellulose tissue [ORC]+polypropylene+absorbable polidioxanon [PDS]); and VI: (a control group without any mesh). Sterile mesh platelets (2 × 2 cm) were implanted into rats and 1.0 × 10⁵ Escherichia coli (ATCC 25922) (in 1 mL) was injected via cannula lateral to each incision. Seventy-two hours later, mesh and tissue samples were obtained and investigated both microscopically and histologically.

Results: In terms of tissue inflammation scores, polypropylene and Proceed meshes yielded significantly higher scores than the control group and the HI-TEX mesh. Upon microbiological evaluation, the HITEX and Proceed mesh groups had significantly higher numbers of E. coli than the other groups.

Conclusions: Polypropylene and ePTFE performed well; the E. coli colonisation rates were low in a rat model of infection. Larger studies are warranted to explore both the short- and long-term utilities of various mesh types placed under infected conditions.

Keywords

Mesh, Infection, Rat.

Introduction

Mesh repair of hernias is the most common operation performed by general surgeons worldwide 6y766. Recurrence rates decreased and patient comfort substantially improved upon introduction of tension-free surgical repair using a prosthetic mesh [1]. However, infection, persistent pain, and recurrence still complicate mesh repair operations [1].

Hernia mesh repair of the anterior abdominal wall is regarded as a clean surgery with an incidence of postoperative mesh infection of approximately 1-2% [2,3]. Wang et al. [4] reported that the rates of surgical-site infections (SSIs), including wound infections, cellulitis, and mesh-related infections, were 3.92-5.1% in patients who were or were not prescribed antibiotic prophylaxis and the meta-analysis of Mazaki et al. [5] reported infection rates of 3.0-6.0%. However, a recent study found that the SSI rate was as high as 13% in patients who underwent elective open mesh-plug hernia repair and did not take any prophylactic antibiotics [6]. In another study on 41 patients who underwent single-stage repair of grade 3 and 4 hernias with biological mesh reinforcement, the overall postoperative wound infection rate was as high as 15% [7]. In light of such data, SSI remains an important problem after hernia repair by mesh insertion.

Staphylococcus aureus and Escherichia coli are the principal microorganisms that infect meshes after surgery; these species can form biofilms on mesh surfaces [8]. Bacterial growth and slime production are influenced by the morphological features of surgical meshes and their hydrophobicity [9]. In fact, synthetic meshes are not recommended for use in patients with active infections because of the high risk of mesh infection [10].

However, few data on the behaviour of synthetic meshes in infected environments are available. In this study, we compared different types of synthetic meshes in terms of the adherence of microorganisms, and identified meshes associated with the lowest levels of infection in an infected environment.

Materials and Methods

The study was conducted in the Experimental Medicine and Investigation Centre of University Medical School. Local Ethics Committee approval was obtained (approval number 2011/22).

Sixty Sprague-Dawley rats (250-320 g) were used. The animals were housed under conditions of constant light (12 h light/dark cycles) and temperature, and received a complete diet of rat food and water ad libitum throughout the entire study. The animals were randomly divided into 6 groups, each of which contained 10 rats, as follows:

• Group I: Prolen Mesh (Ethicon) (polypropylene) group;

• Group II: HI-TEX PARP MP (polyethylene tereoxphthalate [PET]+polyether urethane [PEU]) group;

• Group III: Vypro ll (Ethicon) (polypropylene+polygalactin 910) group;

• Group IV: Motif Mesh (Proxy B?omedical) (expanded polytetrafluoroethylene [ePTFE]; a composite mesh) group;

• Group V: Proceed Mesh (Ethicon) (absorbable oxidised regenerated cellulose tissue [ORC]+polypropylene +absorbable polidioxanon [PDS]) group;

• Group VI: Control group without any mesh.

The animals were intraperitoneally anaesthetised with 50 mg/kg ketamine (Ketalar Flacon, Parke-Davis/Eczaciba?i) and 10 mg/kg xylazin (Rompun, Bayer). After disinfection of the skin with 10% (w/v) povidone iodine (Betadine, Kavcuk Laboratory), a vertical incision 2 cm in length was made paravertebral to the right side. A sterile mesh platelet (2 × 2 cm) was implanted under the skin, muscle, and fascia layers. No antibiotic was administered before or during this procedure. A cannula was inserted lateral to the incision and the end was placed onto the mesh; no fixation material was employed. The skin was closed by stapling. One millilitre (1.0 × 10⁵ bacteria) of an E. coli (ATCC 25922) suspension was injected via the cannula, which was then removed, after which a spray dressing was applied.

The control group underwent identical paravertebral incision and dissection, but no mesh was inserted. This was followed by cannulation lateral to the incision, injection of bacteria, removal of the cannula, and application of a dressing as described above. Seventy-two hours later, all of the animals were intraperitoneally anaesthetised with 50 mg/kg ketamine and 10 mg/kg xylazine. After skin disinfection with 10% (w/v) povidone iodine, the skin clips were removed, the wounds were excised, and the meshes were collected and placed in tubes containing 3 mL sterile saline. The tubes were vortexed and the bacterial suspensions were serially diluted in saline, followed by culture on sheep agar plates for 24 h. Tissue samples (1 cm long) were obtained from the muscle and soft tissue surrounding the meshes (using sterile instruments), fixed in 10% (v/v) buffered formalin, and conventionally processed for histology. Because the control group had no mesh, tissue samples were obtained after standard anaesthesia and sterilisation.

Microbiological evaluation

All of the microbiological evaluations were performed by the same microbiologist who was singly blinded to the test group. Colony-forming units (cfus) were counted if possible; if the colonies were too dense to count, the colony count was considered >300 cfu/mL.

Histopathological evaluation

Specimens were examined by light microscopy (LM) after fixation in 10% (v/v) formalin, embedding in paraffin, and staining of sections with haematoxylin and eosin (H and E). All of the pathological evaluations were performed by the same pathologist who was singly blinded to the test group.

Histopathological evaluation scores

We explored the severity of inflammation. Polymorphonuclear cell migration, mononuclear cell migration, oedema, vasodilation, haemostasis, and increased vascularisation were scored as absent (-), mild (+), moderate (++), or severe (+++); whereas margination and transmigration were scored as absent (-) or present (+). The maximum inflammation score was 20.

Statistical analysis

Histopathological data from the six groups were compared via analysis of Kruskal-Wallis variance. The Mann-Whitney U test was employed for binary group comparisons. The level of statistical significance was set at 0.05. After application of the Bonferroni correction to binary comparisons, the significance level was set at 0.0083. Microbiological data were similarly analysed, but after application of the Bonferroni correction to binary comparisons, the significance level was set at 0.01.

Results

Histopathological results

The lowest histopathological scores were evident in Groups VI and II, and the highest score was observed in Group I as shown in Table 1. Upon binary comparison, the statistical significance level was corrected to P<0.0083. In terms of tissue inflammation scores, the polypropylene and Proceed mesh groups had significantly higher levels of inflammation than the controls and HI-TEX mesh group as shown in Table 2.

Table 1. Histopathological scores of groups.

Table 2. The binary comparison of groups in regards to the level of inflammation.

Microbiological results

Notably, the best microbiological results were evident in Group I, and the poorest results were observed in Groups II and V as shown in Table 3. Upon binary comparisons, the statistical significance level was corrected to P<0.01. Upon microbiological evaluation, the HI-TEX and Proceed mesh groups yielded significantly higher numbers of E. coli colonies than the other groups as shown in Table 4.

Table 3. The colony counts of groups after 24 hours incubation in the incubator.

Table 4. The binary comparison of groups in regards to the microbiological results.

The level of inflammation and their dispersion range, groups vs. degree of inflammation were figured as shown in Figure 1.

Figure 1: The level of inflammation and their dispersion range, groups vs. degree of inflammation.

The dispersion ranges of microbiological results, group vs. colony counts (cfu) were figured as shown in Figure 2.

Figure 2: The dispersion ranges of microbiological results, group vs. colony counts (cfu).

Discussion

We found that Group I (polypropylene) exhibited high-level inflammation, but the lowest number of E. coli colonies, whereas in Group II (PET+PEU), the inflammation level was low, but E. coli numbers were very high. We suggest that in Group I, inflammation was triggered by stimulation of the immune system, which eliminated E. coli. In Group II, the immune system was not stimulated; inflammation was low, but E. coli numbers were high. In Group V (Proceed Mesh; Ethicon; (ORC) +polypropylene+absorbable (PDS)), both the inflammation level and E. coli numbers were high. In Group IV (Motif Mesh; Proxy Biomedical; expanded [ePTFE]; a composite mesh), the inflammation level was not very high, and E. coli numbers were low. Thus, bacteria did not adhere to this mesh, and inflammation was not triggered.

The use of synthetic meshes in infected areas is not advised, and data on the infection rates of synthetic meshes in such environments are limited. Because biological meshes are still not widely available, and since biological graft implantation may require new skills or technical expertise, we determined the infection rates of different types of synthetic mesh in an infected environment. Mesh infections are caused by biofilm formation on mesh surfaces. Apart from microbial properties, mesh structure and hydrophobicity influence the development of infection. Polypropylene is considered to be hydrophobic; this feature prevents initial colonisation by bacteria [11]. Moreover, the additional advantages of polypropylene are that the material is easily handled and quickly integrated. However, multifilament meshes with large surface areas are more prone to biofilm formation. The niches between fibres constitute an environment favourable to microbial development [9].

In a meta-analysis of data from 1,229 patients, the use of biological meshes for ventral hernia repair was shown to reduce the numbers of infectious wound complications compared to the use of synthetic meshes [12]. However, a recent single-centre 3-year outcomes trial evaluated the efficacy of a biological Inguinal Hernia Matrix (IHM; Cook Medical) compared to a polypropylene mesh. One hundred patients were included. No infection was found in any case, and no between-group difference was evident in terms of SSI [1]. Another study collected data on 41 patients who underwent removal of infected or exposed meshes, followed by singlestage reconstruction of the abdominal wall using a synthetic mesh (polypropylene). The short-term post-operative course was uneventful in 27 patients; 6 (14.6%) developed minor wound infections and were treated with dressings and antibiotics; 5 (12%) developed wound infections requiring debridement; and 1 required complete mesh removal. The cited authors concluded that standard polypropylene mesh could be used to place only grafts in infected surgical fields [13]. In the study by Souza et al. [14] in 100 patients, the use of uncoated mid-weight polypropylene mesh to re-in force midline ventral hernia repairs was not associated with increased rates of infection, fistula formation, or clinically significant adhesions.

PEU promotes the synthesis of high levels of both IL-6 and TNF-α [15]. Our PEU group (Group II) did not exhibit highlevel inflammation, but E. coli numbers were elevated. Thus, the polyethylene PET+PEU mesh may be unsuitable for use in infected environments. Although the mesh of Group V can be absorbed, high-level inflammation and high E. coli numbers were evident; thus, this mesh may be unsuitable for use in infected environments. Interestingly, and in line with our results, a prospective study comparing different mesh types found that inflammatory cell numbers were higher around polypropylene meshes compared to those containing both polypropylene and oxidised regenerated cellulose [16]. In another study, Parietene Composite^® (polypropylene coated with absorbable collagen) and Omyramesh^® (condensed polytetrafluoroethylene [cPTFE]) performed well in a contaminated environment [17]. Some study limitations must be mentioned. First, the time allowed for microbial adhesion, 72 h, was rather short, and we did not record late infections. Secondly we did not evaluate any biological mesh.

In conclusion, polypropylene and ePTFE meshes performed well. The E. coli colonisation rates were low in an infected rat model environment. Larger studies are warranted to explore the short- and long-term outcomes associated with the use of different mesh types under infected conditions.

References

1. Bochicchio GV, Jain A, McGonigal K, Turner D, Ilahi O, Reese S. Biologic vs. synthetic inguinal hernia repair: 1-year results of a randomized double-blinded trial. J Am Coll Surg 2014; 218: 751-757.
2. Engelsman AF, van der Mei HC, Ploeg RJ, Busscher HJ. The phenomenon of infection with abdominal wall reconstruction. Biomaterials 2007; 28: 2314-2327.
3. Ioannidis O, Paraskevas G, Varnalidis I, Ntoumpara M, Tsigkriki L, Gatzos S. Hernia mesh repair of the anterior abdominal wall and antibiotic chemoprophylaxis: multiple doses of antibiotics failed to prevent or reduce wound infection. Chirurgia 2013; 108: 835-839.
4. Wang J, Ji G, Yang Z, Xi M, Wu Y, Zhao P. Prospective randomized, double-blind, placebo controlled trial to evaluate infection prevention in adult patients after tension-free inguinal hernia repair. Int J Clin Pharmacol Ther 2013; 51: 924-931.
5. Mazaki T, Mado K, Masuda H, Shiono M. Antibiotic prophylaxis for the prevention of surgical site infection after tension-free hernia repair: a Bayesian and frequentist meta-analysis. J Am Coll Surg 2013; 217: 788-801.
6. Mazaki T, Mado K, Masuda H, Shiono M, Tochikura N, Kaburagi M. A randomized trial of antibiotic prophylaxis for the prevention of surgical site infection after open mesh-plug hernia repair. Am J Surg 2014; 207: 476-484.
7. Sbitany H, Kwon E, Chern H, Finlayson E, Varma MG, Hansen SL. Outcomes Analysis of Biologic Mesh Use for Abdominal Wall Reconstruction in Clean-Contaminated and Contaminated Ventral Hernia Repair. Ann Plast Surg 2015; 75: 201-204.
8. Reslinski A, Dabrowiecki S. Evaluation of the effect of glucose on Staphylococcus aureus and Escherichia coli biofilm formation on the surface of polypropylene mesh. Medycyna doswiadczalna i mikrobiologia 2013; 65: 19-26.
9. Engelsman AF, van der Mei HC, Busscher HJ, Ploeg RJ. Morphological aspects of surgical meshes as a risk factor for bacterial colonization. Br J Surg 2008; 95: 1051-1059.
10. Mariette C, Briez N, Denies F, Dervaux B, Duhamel A, Guilbert M. Use of biological mesh versus standard wound care in infected incisional ventral hernias, the SIMBIOSE study: a study protocol for a randomized multicenter controlled trial. Trials 2013; 14: 131.
11. Karakecili AG, Gumusderelioglu M. Comparison of bacterial and tissue cell initial adhesion on hydrophilic/hydrophobic biomaterials. J Biomater Sci Polym Ed 2002; 13: 185-196.
12. Darehzereshki A, Goldfarb M, Zehetner J, Moazzez A, Lipham JC, Mason RJ. Biologic versus nonbiologic mesh in ventral hernia repair: a systematic review and meta-analysis. World J Surg 2014; 38: 40-50.
13. Birolini C, de Miranda JS, Utiyama EM, Rasslan S. A retrospective review and observations over a 16-year clinical experience on the surgical treatment of chronic mesh infection. What about replacing a synthetic mesh on the infected surgical field? Hernia 2014; 19: 239-246.
14. Souza JM, Dumanian GA. Routine use of bioprosthetic mesh is not necessary: a retrospective review of 100 consecutive cases of intra-abdominal midweight polypropylene mesh for ventral hernia repair. Surg 2013; 153: 393-399.
15. Rodriguez A, Meyerson H, Anderson JM. Quantitative in vivo cytokine analysis at synthetic biomaterial implant sites. J Biomed Mater Res A 2009; 89: 152-159.
16. Harrell AG, Novitsky YW, Cristiano JA, Gersin KS, Norton HJ, Kercher KW. Prospective histologic evaluation of intra-abdominal prosthetics four months after implantation in a rabbit model. Surg Endosc 2007; 21: 1170-1174.
17. Deerenberg EB, Mulder IM, Grotenhuis N, Ditzel M, Jeekel J, Lange JF. Experimental study on synthetic and biological mesh implantation in a contaminated environment. Br J Surg 2012; 99: 1734-1741.