Association of methylation and expression of IL-4 and IFN-γ in peripheral blood of asthmatic patients
- *Corresponding Author:
- Li Gao
Department of Chinese and Western Medicine
College of Traditional Chinese Medicine
Xinjiang Medical University
Urumqi, PR China
Xinjiang Medical University
Urumqi, PR China
Accepted on June 15, 2017
This study aims to compare the expressions and methylation of interleukin-4 (IL-4) and interferon-γ (IFN-γ) between Han and Uighur populations as well as their relationships with asthma. A total of 108 asthmatic patients (including 73 Han patients and 35 Uyghur patients) were included into this study, together with 150 healthy populations (including 120 Han patients and 30 Uyghur patients) as the control. The levels of IL-4 and IFN-γ were measured by Polymerase Chain Reaction (PCR), their protein expressions were detected by Enzyme-Linked Immuno Sorbent Assay (ELISA), and their methylation degrees were detected by SNaPshot. Compared with the control group, the expressions of IL-4 and IFN- γ genes and proteins were increased in the asthma group (P<0.05); the methylation degree of IL4-1SF locus was higher (P<0.05); the analysis toward the asthma group showed that the expressions of IL-4 and IFN-γ genes in the Uyghur patients were slightly higher while their protein expressions were significantly decreased; however, there was no significant difference in the methylation level of IL-4 and IFN-γ among different loci between the Han and Uyghur patients (P>0.05). IL-4 and IFN-γ are overexpressed in asthmatic patients, especially in Han patients. The methylation degree of IL4-1SF locus in asthmatic patients is increased, but there is no difference between Han and Uyghur populations.
Asthma, Epigenetics, Gene expression, Methylation.
Bronchial asthma (abbreviated as asthma hereinafter) is a chronic inflammation in the respiratory tract with hallmarks as mucous metaplasia and airway remodelling, and recognized as one of the chronic lung diseases that threaten human health around the world . The incidence in adults is about 5%-6%, and shows an increasing trend year by year . Like other allergic diseases, asthma is a multifactorial disease, including environment and heredity, which means the onset of asthma is not only related to genetic factors but also closely related to the immune, with the degree of heritability about 80% [3-5].
Until now, we know that the immune imbalance of Th1/Th2 cytokines is one of the key mechanisms of this disease. IL-4 and IFN-γ are the main cytokines secreted by the Th2 and Th1 cells . Nowadays, it’s considered that IL-4 and IFN-γ-caused imbalance of Thl and Th2 is a key factor in the pathogenesis of asthma, which are both associated with the severity of asthma . Different races, ethnic groups, and populations have some differences in disease susceptibility and resistance . Previous studies found that the incidence of allergic rhinitis in Han populations was 33.36% and 19.19% in Uighur populations . In addition, 25.58% of Uighur asthmatic patients have family history, which is also lower than that in China (about 40% of asthmatic patients have family history) [10,11].
Meanwhile, the epigenetic mechanisms have been shown to regulate many genes including those involved in inflammation and the immune response and to ensure inheritance of phenotype with cell division . For example, DNA methylation, which regulates the expressions of immune system-related genes, also modifies the development of the innate and adaptive immune responses . In this study, SNaPshot typing technique was used to quantitatively analyse 5 adjacent CpG loci of IL-4 and IFN-γ in 105 asthmatic patients and 105 healthy populations, aiming to investigate the difference of epigenetics in different races and its role in the pathogenesis of asthma.
Materials and Methods
The asthmatic patients treated in the First Affiliated Hospital of Xinjiang Medical University and the People’s Hospital of Xinjiang Uygur Autonomous Region from Dec 2014 to Jul 2015 was enrolled.
Inclusion criteria: (1) Aging 18-60 y old; (2) With a disease history ≥ 1 y; (3) Had lived locally in Xinjiang for at least 5 y and had not long-term lived in outer regions; (4) Met with the diagnostic criteria of bronchial asthma prevention and treatment guidance (2008): recurrent wheezing, shortness of breath, chest tightness or cough, which was more related to the exposure to allergens, cold air, physical or chemical stimuli, viral upper respiratory tract infection, or exercise; scattered or diffuse expiratory phase-based wheezing can be heard in the lungs at the onset; the expiratory phase was prolonged; the patients with atypical clinical symptoms should have at least one positive result of the following tests: bronchial provocation test or exercise test, bronchodilation test (FEV1 was increased by more than 15%, and the absolute value of FEV1 was increased by >200 ml), intraday variability or circadian volatility of PEF ≥ 20%; 5) signed the informed consent.
Exclusion criteria: (1) with other airway infectious diseases; (2) With other allergic diseases; (3) With hypertension, diabetes, or other metabolic disorders; (4) Administrated immunosuppressive agents within 1 week. A total of 150 asthmatic patients that met with the above criteria were enrolled in this study, including 120 Han patients and 30 Uygur patients, with a mean age as (35.81 ± 11.37) y. A total of 108 healthy population with normal physical examination results were included as the control group, with the mean age as (37.21 ± 10.62) y. This study was conducted in accordance with the declaration of Helsinki. This study was conducted with approval from the Ethics Committee of Xinjiang Medical University. Written informed consent was obtained from all participants.
Polymerase chain reaction (PCR)
All patients were sampled with 5 ml blood, and the CD4+ lymphocytes were collected by magnetic activated cell sorting (MACS) from 4 ml, then IL-4 mRNA and IFN-γ mRNA were extracted by Trizol and the expression level of both were detected after PCR amplification .
Enzyme-linked immuno sorbent assay (ELISA)
The protein expression of IL-4 and IFN-γ were detected by ELISA (Xin Le Biological Engineering Co. Ltd., Shanghai, China) based on the plasma used for PCR detection .
The remain blood (1 ml) was used to extract DNA after cell lysis, and the methylation were detected by EZ DNA methylation modification Kit (ZYMO RESEARCH Biotechnology company, Orange County, California, USA) .
SPSS17.0 was used for the statistical analysis; the mRNA expression levels (IFN-γ, IL-4 and GAPDH) were expressed as mean ± standard deviation (x? ± s); the computed tomography (CT) values and protein expression levels were analysed using the t-test of two independent samples, with P<0.05 considered as statistical significance. As for the methylation data, the raw data collected from the ABI3730XL sequencer were analysed by GeneMapper (Applied Bio systems Co. Ltd., USA) and SPSS17.0; the methylation levels of IFN-γ and IL-4 were expressed as mean ± standard deviation (x? ± s)%; the comparison of the methylation degree between two genes was compared using the t-test of two independent samples, with P<0.05 considered as statistical significance.
Expressions of IL-4 and IFN-γ
Compared with the control group, the levels of IL-4 and IFN-γ mRNA in the asthmatic patients were slightly increased, but the differences were statistically significant (P<0.05). The protein expressions of IL-4 and IFN-γ were similar to those of the mRNAs (P<0.05) (Table 1).
|Items||Asthma group (n=150)||Control group (n=108)||P value|
|IL-4 mRNA||10.54 ± 2.41||9.95 ± 1.91||0.047|
|IFN-γ mRNA||8.33 ± 2.97||6.86 ± 1.80||0|
|IL-4 protein||2107.74 ± 611.75||444.19 ± 138.87||0|
|IFN-γ protein||394.59 ± 134.40||319.08 ± 110.30||0|
Table 1. IL-4/IFN-γ expression in asthma group and control group (?x ± s).
Compared with the Han asthmatic patients, the mRNA levels of IL-4 and IFN-γ in Uygur asthmatic patients were statistically increased in the Uygur asthmatic patients (P<0.05), but the protein and mRNA expression levels were lower; there was no significant difference in the IL-4 protein between the two groups (P=0.219), but the expression of IFN-γ protein exhibited statistical difference (P=0.004) (Table 2).
|Items||Uygur race (n=30)||Han race (n=120)||P value|
|IL-4 mRNA||10.69 ± 2.99||9.74 ± 1.88||0.038|
|IFN-γ mRNA||9.62 ± 3.57||8.14 ± 2.75||0.029|
|IL-4 protein||1961.78 ± 530.88||2121.92 ± 627.40||0.219|
|IFN-γ protein||264.28 ± 97.23||331.69 ± 111.96||0.004|
Table 2. IL-4/IFN-γ expression in asthma patients in different races (?x ± s).
Methylation levels of IL-4 and IFN-γ
Compared with the control group, the methylation degree of IL4-1SF locus in the asthmatic patients was significantly increased (P=0.040), but other loci, namely IFN-γ1SF, IFN- γ2SR, IL4-2SR, and IL-44SR showed no significant difference in the methylation degree (P>0.05) (Table 3).
|Items||Asthma group (n=150)||Control group (n=108)||P value|
|IL-41SF||97.04 ± 5.13||92.30 ± 15.88||0.04|
|IL-42SR||91.05 ± 1.78||90.75 ± 2.02||0.256|
|IL-44SR||93.58 ± 1.54||93.44 ± 1.56||0.515|
|IFN-γ1SF||94.87 ± 1.17||95.14 ± 9.02||0.066|
|IFN-γ2SR||96.53 ± 6.53||96.59 ± 7.28||0.517|
Table 3. Methylation sights of IL4-1SF, IL4-2SR, IL-44SR, IFN-γ1SF and IFN-γ2SR in asthma group and control group (?x ± s,%).
Compared with the Han asthmatic patients, the methylation levels of IL-4 and IFN-γ loci in the Uighur asthmatic patients were similar, showing no statistical significance (P>0.05) (Table 4).
|Items||Uygur race (n=30)||Han race (n=120)||P value|
|IL-41SF||96.32 ± 7.43||97.29 ± 4.04||0.392|
|IL-42SR||90.99 ± 2.02||91.07 ± 1.71||0.826|
|IL-44SR||93.79 ± 1.75||93.50 ± 1.46||0.389|
|IFN-γ1SF||95.12 ± 0.78||95.13 ± 0.93||0.905|
|IFN-γ2SR||96.48 ± 0.89||96.55 ± 0.54||0.621|
Table 4. Methylation sights of IL4-1SF, IL4-2SR, IL-44SR, IFN-γ1SF and IFN-γ2SR in Han and Uygur patients (?x ± s,%).
One study of Taiwan reveals that the prevalence of asthma has increased from 12.99%-16.86%, and environmental changes are suspected to be the major driver of asthma with air pollution identified as an important exposure, but it is still a complex genetic trait meaning that the phenotype is the result of the interaction of multiple genes [17-20].
This study revealed that the mRNA and protein levels of IL-4 in the asthma group were significantly higher than the healthy control, confirming that asthma is mainly characterized by Th2 hyperthyroidism-based immune responses, suggesting that IL-4 antagonists can be used clinically as targeted therapy against asthma, which can interrupt the IL-4 signal pathway, thereby reducing the number of pro-inflammatory factors in the airway and improving patients’ conditions . IL-4 can promote the secretion of Th2 cells by reducing the secretion of IFN-γ, which is the main process that medicates the pathological and physiological changes of asthma. Compared with the Han asthmatic patients, the IL-4 mRNA expression was slightly elevated in the Uighur asthmatic patients, but there was no difference in the IL-4 protein expression, suggesting that IL-4 may not be the reason that causes the different incidences of asthma in Han and Uighur populations.
IFN-γ is an important Th1 cytokines, can inhibit the release of Th2 cytokines, and can also regulate the immune function . Studies have shown that one of the important reasons for allergic asthma in children is the reduction of Th1-type cytokines . IFN-γ can inhibit the expression of IL-4 gene and the synthesis of IgE by IL-4, thus participating in the occurrence and development of inflammatory processes in asthma , and it can also affect the proliferation and differentiation of Th2 and Th1 as well as antagonize the role of IL-4. Nowadays, current studies have confirmed that the Th1 cells can produce not only IL-2 but also IFN-γ . Compared with IL-4, the Th1 cytokine, IFN-γ, was also highly expressed in the asthma group than the control group, not consistent with the recognized Th1 immune response reduction-based pathogenesis of asthma, and it may be caused the secretion differences of IFN-γ at the transcription level and the translation level.
However, in recent years, Th1 and Th2 have been found to be able to induce, instead of mutually antagonizing, the activity of bronchial asthma, respectively, which means the existence of mutual promotion between these two . The fact that the IFN-γ gene and protein expressions were increased in the asthma group can be interpreted as the results of the mutual promotion between Th1 and Th2 rather than mutual antagonism.
However, further studies are still needed to verify these different results. Further analysis targeting the Han and Uygur asthmatic patients showed that although the IFN-γ mRNA level in the Uygur asthmatic patients was slightly higher than the Han asthmatic patients, the protein expression level was significantly reduced, suggesting that the difference in the plasma concentration of this factor may be the reason leading to the difference in the pathogenesis of asthma between Han and Uygur populations.
The results of SNPshot typing showed that the methylation degree of IL-41SF locus in the asthma group was significantly higher than the control group. It is generally believed that the regulatory role of DNA hyper methylation is associated with gene silencing, suggesting that the hyper methylation of IL-41SF is closely related to the occurrence of asthma, so it can be used to find new treatment directions against asthma. However, because there was no significant difference in the methylation degrees of IL-4 and IFN-γ between the Uygur and Han asthmatic patients, it suggests that the methylation degree may not be the cause resulting in the different prevalence between Uygur and Han patients; or it may be caused by too small Uygur patients, which needs to further expand the sample size so as to verify the results.
This study was supported by the National Natural Science Foundation of China (NDRC), project No.8126006 (2013, 01-2016, 12).
Conflicts of Interest
The authors declare no conflict of interest.
- Habibovic A, Hristova M, Heppner DE, Danyal K, Ather JL, Janssen-Heininger YM, Irvin CG, Poynter ME, Lundblad LK, Dixon AE, Geiszt M, van der Vliet A. DUOX1 mediates persistent epithelial EGFR activation, mucous cell metaplasia, and airway remodelling during allergic asthma. JCI Insight 2016; 1: e88811.
- Bateman ED, Hurd SS, Barnes PJ, Bousquet J, Drazen JM, FitzGerald M, Gibson P, Ohta K, O'Byrne P, Pedersen SE, Pizzichini E, Sullivan SD, Wenzel SE, Zar HJ. Global strategy for asthma management and prevention: GINA executive summary. Eur Respir J 2008; 31: 143-178.
- Ji H, Biagini Myers JM, Brandt EB, Brokamp C, Ryan PH, Khurana Hershey GK. Air pollution, epigenetics and asthma. Allergy Asthma Clin Immunol 2016; 12: 51.
- Sanz-LCS, Garcia-SV, Davila I, Isidoro-GM. Applications of Molecular Genetics to the Study of Asthma. Methods Mol Biol 2016; 1434: 1-13.
- Masoli M, Fabian D, Holt S, Beasley R. Global Initiative for Asthma (GINA) Program. The global burden of asthma: executive summary of the GINA Dissemination Committee report. Allergy 2004; 59: 469-478.
- Gerasimova A, Chavez L, Li B, Seumois G, Greenbaum J, Rao A, Vijayanand P, Peters B. Predicting cell types and genetic variations contributing to disease by combining GWAS and epigenetic data. PLoS One 2013; 8: e54359.
- Yoshimoto T, Tsutui H, Nakanishi K. Importance of Th17 and Th1 associated responses for the development of asthma. Inflammation Allergy Drug 2011.
- Newman-TA. Environmental determinants of asthma. Lancet 1995; 345: 296-299.
- Melen E, Pershagen G. Pathophysiology of asthma: lessons from genetic research with particular focus on severe asthma. J Intern Med 2012; 272: 108-120.
- Lopez KI, Martinez SE, Moguel MC, Romero LT, Figueroa CS, Pacheco GV, Ibarra B, Corona JS. Genetic diversity of the IL-4, IL-4 receptor and IL-13 loci in mestizos in the general population and in patients with asthma from three subpopulations in Mexico. Int J Immunogenet 2007; 34: 27-33.
- McGeachie MJ, Dahlin A, Qiu W, Croteau-Chonka DC, Savage J, Wu AC, Wan ES, Sordillo JE, Al-Garawi A, Martinez FD, Strunk RC, Lemanske RF Jr, Liu AH, Raby BA, Weiss S, Clish CB, Lasky-SJA. The metabolomics of asthma control: a promising link between genetics and disease. Immun Inflamm Dis 2015; 3: 224-238.
- Barnes PJ. Targeting the epigenome in the treatment of asthma and chronic obstructive pulmonary disease. Proc Am Thorac Soc 2009; 6: 693-696.
- Suarez-AB, Baragano RA, Ortega F, Lopez-LC. Epigenetic modulation of the immune function: a potential target for tolerance. Epigenetics 2013; 8: 694-702.
- Wu S, Wang G, Yang R, Cui Y. Anti-inflammatory effects of Boletus edulis polysaccharide on asthma pathology. Am J Transl Res 2016; 8: 4478-4489.
- Choi J, Choi BK, Kim JS, Lee JW, Park HA, Ryu HW, Lee SU, Hwang KW, Yun WK, Kim HC, Ahn KS, Oh SR, Lee HJ. Picroside II attenuates airway inflammation by down regulating the transcription factor GATA3 and Th2-related cytokines in a mouse model of HDM-induced allergic asthma. PLoS One 2016; 11: e0167098.
- Kwon NH, Kim JS, Lee JY, Oh MJ, Choi DC. DNA methylation and the expression of IL-4 and IFN-gamma promoter genes in patients with bronchial asthma. J Clin Immunol 2008; 28: 139-146.
- Ma YC, Lin CC, Li CI, Chiang JH, Li TC, Lin JG. Time-trend analysis of prevalence, incidence and traditional Chinese medicine use among children with asthma: a population-based study. J Public Health 2016; 38: 263-271.
- Gehring U, Cyrys J, Sedlmeir G, Brunekreef B, Bellander T, Fischer P, Bauer CP, Reinhardt D, Wichmann HE, Heinrich J. Traffic-related air pollution and respiratory health during the first 2 years of life. Eur Respir J 2002; 19: 690-698.
- Takizawa H. Impact of air pollution on allergic diseases. Korean J Intern Med 2011; 26: 262-273.
- Begin P, Nadeau KC. Epigenetic regulation of asthma and allergic disease. Allergy Asthma Clin Immunol 2014; 10: 27.
- Quirce S, Bobolea I, Barranco P. Emering drugs for asthma. Expert Opin Emerg Drugs 2012; 17: 219-237.
- Michel S, Busato F, Genuneit J, Pekkanen J, Dalphin JC, Riedler J, Mazaleyrat N, Weber J, Karvonen AM, Hirvonen MR, Braun-Fahrlander C, Lauener R, von Mutius E, Kabesch M, Tost J. Farm exposure and time trends in early childhood may influence DNA methylation in genes related to asthma and allergy. Allergy 2013; 68: 355-364.
- Renzi PM, Turgeon JP, Marcotte JE, Drblik SP, Berube D, Gagnon MF, Spier S. Reduced interferon-gamma production in infants with bronchiolitis and asthma. Am J Respir Crit Care Med 1999; 159: 1417-1422.
- Reinius LE, Gref A, Saaf A, Acevedo N, Joerink M, Kupczyk M, D’Amato M, Bergstrom A, Melen E, Scheynius A, Dahlen SE, Pershagen G, Soderhall C, Kere J. DNA methylation in the Neuropeptide S Receptor 1 (NPSR1) promoter in relation to asthma and environmental factors. PLoS One 2013; 8: e53877.
- Runyon RS, Cachola LM, Rajeshuni N, Hunter T, Garcia M, Ahn R, Lurmann F, Krasnow R, Jack LM, Miller RL, Swan GE, Kohli A, Jacobson AC, Nadeau KC. Asthma discordance in twins is linked to epigenetic modifications of T cells. PLoS One 2012; 7: e48796.
- Huang HR, Zhong YQ, Wu JF. The association between IFN-γ and IL-4 genetic polymorphisms and childhood susceptibility to bronchial asthma. Gene 2012; 494: 96-101.