- Biomedical Research (2015) Volume 26, Issue 2
Acute effects of triterpene compounds on locomotor performance and Morris water maze tasks in Spraque-Dawley rats.Nasir MN1,4, Habsah M3, Adzim MKR1,2*, Norhayati AH1,2, Muralidhara DV1, Zubaidi AL1
- *Corresponding Author:
- Mohd Adzim Khalili bin Rohin
Faculty of Health Sciences,
Universiti Sultan Zainal Abidin,
Gong Badak Campus
21300 Kuala Terengganu
Accepted date: December 04 2014
Centella asiatica has been recognized as one of the plants that can be used to surge cognitive function in animals and humans. Currently, several compounds isolated from C. asiatica have been proven to enhance cognitive function via learning and memory properties. The present study investigated the acute effects of administration of triterpene compounds isolated from C. asiatica on memory and learning in male Spraque-Dawley rats via Morris water maze and their influences on motor activity in locomotor performance. The five-week old Spraque-Dawley rats were intraperitoneally administered with 10 mg/kg concentration of triterpene compounds; asiatic acid, madecassic acid, madecassoside and the control groups with scopolamine and saline. The rats were then evaluated via Morris water maze tasks and locomotor activity. The administration of 10 mg/kg of asiatic acid resulted in significantly improved memory (p<0.5) with increased escaped latency compared to saline and scopolamine. Furthermore, the administration of 10 mg/kg of madecassic acid and madecassoside significantly induced higher escape latency compared to scopolamine (p<0.5) but there was no significant difference compared to saline (p<0.5). For spontaneous locomotor activity, the three triterpene compounds showed no effect on locomotor activity as compared to saline group. However, the triterpene compounds had significant effect on locomotor activity as compared to scopolamine group (p<0.5). The administration of acute asiatic acid facilitated escaped latency which can be translated as having the properties of enhancement on memory and learning. Hence, it may serve to be useful on improving memory and learning with no sedative effect on the locomotor performance.
Asiatic Acid, Madecassic Acid, Madecassoside, Morris Water Maze, Locomotor Activity
Natural products such as herbal medicines have been used for centuries as an alternative medicine in treating and preventing neurodegenerative diseases including Alzheimer’s disease (AD). There is an unambiguous relationship between AD and natural products. The most consistent features of AD are cholinergic deficits , decreased acetylcholine (ACh) production and also enhanced levels of acetylcholinesterase (AChE). Due to that, treatment strategies have been focusing on replacement therapy for deficits in central cholinergic neurotransmission [2,3] which result in loss of ability to recall memory and process of formation of new memory and learning.
Over the past decade most research in AD has emphasized the use of natural products including Centella asiatica (L.) Urb. (Apiaceae). C. asiatica is a slender and creeping perennial herbal plant with weak aroma used by diverse ancient people from different cultures in Malaysia and other Asian countries. C. asiatica is considered as a vital herb in Ayurveda, the traditional science of health in India, used to stimulate learning and memory . Previous studies demonstrated that whole plants of C. asiatica have been shown to be beneficial in improving memory . These results support that C. asiatica can enhance memory and learning. C. asiatica contains triterpenes, essential oils, amino acids and other compounds, such as vellarin. The triterpenes include asiaticoside, centelloside, madecassoside, brahmoside, brahminoside, thankuniside, centellose, brahmic, centellic, madecasic acids and asiatic acid  as shown in Figure 1.
A recent study reported that asiatic acid has been shown to improve memory via passive avoidance study and learning capabilities via active avoidance . One the one hand, madecassoside has been indicated as causing neuroprotective effect in a rat’s brain  and most impairment observed there will affect a significant cognitive learning and memory deficit . On the other hand, antioxidant activity has been proven to cause cognitiveenhancing activities  and C. asiatica is consistent with higher corresponding to antioxidant activity . Thus far, however, most studies in the field of C. asiatica have only focused on the extracts rather than the compounds. Little is known about the compounds of C. asiatica and it is not clear what factors on the learning and memory. Therefore, the aims of the present study were to investigate the effects of intraperitoneal (I.P) administration of asiatic acid, madecassic acid and madecasosside on memory and learning along with the spontaneous locomotor activity in male rats.
Materials and Methods
Sample preparation and animals and drugs preparation
C. asiatica whole plants were obtained from a local producer in Kuala Terengganu, Terengganu Darul Iman, Malaysia. Then, the samples were subjected to correct botanical identification by a herbarium expert from Universiti Sultan Zainal Abidin (UniSZA). A voucher specimen is deposited at Biochemistry Laboratory, Faculty of Medicine and Health Science as CA 100. The samples were collected and weighted. Thereafter, the whole plants were grounded with an electric blender (Blender Waring Commercial) to obtain powder form. The grounded samples then underwent an extraction phase.
Extraction and solvent partitioning
The powder samples were extracted with methanol (MeOH) using hot extraction with Soxhlett method. Thereafter, the extractions were repeated three times to ensure the entire bioactive compound was extracted from the sample as previously reported .
The extracts then underwent solvent partitioning technique with n-hexane and chloroform. All soluble fractions in two different solvent systems were screened and subjected to thin layer chromatography (TLC) profiling with reference compound to determine the prominent soluble fraction. p-Anisaldehyde–sulfuric acid mixture were used as visualizing agent in detecting triterpene compounds.
Preparation of column chromatography (CC) was performed as previously described . Briefly, the column (35.0 x 6.5 cm) was filled with silica gel (Merck 9385). Silica gel was mixed with solvent to obtain a slurry mixture. The mixture was stirred slowly to eliminate air bubbles and filled into the column. The column was tapped constantly while packing with the slurry mixture of silica gel. The column was stabilized with the lowest polar of the desired solvent system. The samples were impregnated to silica gel (1:1) and were introduced to the top of the silica gel bed.
The elution process was carried out in increasing polarity (step gradient). The fractions were collected in 100 ml each (first CC process) and 5 ml each (for repeated CC process). The fraction of interest was monitored by TLC. Fractions with the same TLC pattern/profile were combined. The fraction containing the same chemical compound was purified by repeating CC (70.0 x 1.0 cm). The compounds were identified using Infrared spectroscopy (IR spectroscopy) and co-TLC with standard.
Male Spraque-Dawley rats (4-5 weeks) weighing 180 ± 30 g were obtained from Laboratory Animal Unit, Universiti Sains Malaysia and were bred at the animal house, Faculty of Medicine and Health Sciences. The Spraque- Dawley rats were used to analyze the acute effect of treatment with asiatic acid, madecassic acid and madecassoside via I.P injection. It was observed that male and female animals differ in their memory capacities. Estrogen has been found to enhance memory in female mice and women . Therefore, male animals were employed to eliminate estrogen-related beneficial effects on memory. The rats were kept four per cage at room temperature of 22–24 0C and with a 12-h light 12-h dark cycle light period, 0700–1900 h. The animals had free access to food and water during the experiments. The animals were divided randomly into five subgroups of seven animals each namely negative control group, positive control and treated group. Negative control group were given 1 ml/kg saline whereas treated groups animals were given (10 mg/kg) via I.P of three triterpene compounds. The 10 mg/kg administrations were chosen due to the limited compound samples available and as starting point of the comparison of the three compounds. For positive control, the animals were given 3 mg/kg scopolamine to induce amnesia and hyperactivity in locomotor activity. Previous study indicated that 100 to 1000 mg/kg C.asiatica did not produce toxicity effects; breathing, cutaneous effects, sensory nervous system responses . All experiments were conducted at the same time of the day to minimize cir cadian influence. The experimental protocols were approved by Ethical Committee on the Use and Care of Animals (Animal Ethics Committee, Universiti Sultan Zainal Abidin, UniSZA: UniSZA/AEC/2013/001).
Asiatic acid, madecassic acid and madecassoside were isolated from C. asiatica extract; scopolamine was purchased from Sigma Chemical Co. (St. Louis, MO, USA).
Drugs preparation and administration
Asiatic acid, madecassic acid and madecassoside were dissolved in saline with some modification  and scopolamine was dissolved in saline and vortex in 10 minutes. The three triterpene compounds were injected via I.P as well as scopolamine [17,18]. The I.P administration was chosen in view that it allows quite long longer period of absorption as rapid as intravenous route and it is a common route in animal studies .
Spontaneous locomotors activity
The open-field test was used for estimation of locomotor activity of rats using the SMART video tracking software (3.0.03) from Panlab, Spain. The locomotor activity was determined by performing the method as described by . Briefly, the apparatus consisted of a square of 100 cm × 100 cm black floor. A single rat was placed inside the apparatus for 1 min of adaptation. The total length of crossings was 5 min. Asiatic acid, madecassic acid and madecassoside, scopolamine and saline were administered 30 min before the test.
Morris water maze
The Morris water maze task was performed as previously described [20,21]. The Morris water maze is a black circular pool (150cm in diameter and 65cm in height) with a featureless inner surface. The circular pool was filled with water to a height of 30 cm (20±1 ◦C). The pool was divided into four quadrants of equal area. The hidden circular platform (10 cm in diameter), made of Plexiglas, is located in the center of the southeast quadrant, submerged 1.5 cm beneath the surface of the water. It has been previously shown that the Plexiglas is invisible for the rats. The continuous location of each swimming mouse, from the start position to the platform, was monitored by a SMART video tracking software (3.0.03).
In the water maze experiments, the day prior to the experiment was dedicated to swim training for 60 s in the absence of the platform. During the following days, the rats were given two trial sessions each day for 4 consecutive days. During each trial, the time taken to swim to the platform (escape latency) was recorded. This parameter was averaged for each session of trials and for each rat. Once a rat located the platform, it was permitted to remain on it for 15 s. If the rat did not locate the platform within 120 s, it was placed on the platform for 15 s and then removed from the pool by the experimenter (trial 1). The rat was given second trial (trial 2) with an inter-trial interval of 20 min for 4 consecutive days. The point of entry of the rat into the pool and the location of the platform for escape remained unchanged between trial 1 and trail 2 but was changed each day thereafter.
The decrease in escape latency from day to day in trial 1 represents long-term memory or reference memory, while the decrease from trial 1 to trial 2 represents shortterm memory or working memory. Seven rats were used per treatment. The rats were treated with Asiatic acid, madecassic acid and madecassoside (10 mg/kg body weight) given before the training trial. After 90 min, amnesia was induced in rats with scopolamine (3 mg/kg body weight) given subcutaneously. All rats were tested for spatial memory 30 min after the administration of scopolamine.
Results were expressed as means ± SEM of seven animals per group. The data was analyzed with Kruskall-Wallis and followed by Mann-Whitney and 1-way ANOVA using the SPSS 11.0 software. In all the tests, the criterion for statistical significance was p < 0.05.
Extraction and isolation of triterpene compounds
The dried and finely ground plants of C. asiatica (15 kg) were extracted at room temperature with MeOH for three days and were concentrated under vacuum to afford 345 g of crude extract. The extract (200 g) was dissolved in water and successively extracted with n-hexane and chloroform to yield, respectively, 85 g and 70 g of fraction.
The hexane and chloroform fractions were compared via TLC and n-hexane fraction was chosen due to more desired terpenoids were concentrated in this fraction. The nhexane fraction were subjected to a CC filled with silica gel (63–200, 60 A°) and eluted with a gradient of nhexane in dichloromethane and dichloromethane in methanol. Eighty-eight fractions of 100 ml were collected and regrouped on the basis of analytical (TLC) in fifty fractions. The main active fraction of 12 g was subjected to repeated column chromatography (60 cm×3 cm) filled with silica gel (32–63, 60 A°) eluted with a gradient of methanol in chloroform to yield 3 compounds.
IR spectroscopy spectrum for asiatic acid, madecassic acid and madecassoside were done using Perkin Elmer spectrum 100 FTIR spectrometer and the data were compared . The compounds were isolated as a white amorphous powder and identified as asiatic acid (80 mg), madecassic acid (65 mg) and madecassoside (54 mg). The identity of compound was also determined by co-TLC with standard.
Spontaneous locomotors activity
Spontaneous locomotor activity results are shown in Figure 2. Based on the results, the rats from scopolamine group showed statistically significant hyperactivity compared to saline group. For asiatic acid, madecassic acid and madecassoside with each 10 mg/kg the groups showed no effect on locomotor activity as compared to saline group.
Morris water maze
The Morris water maze test results were shown in Figure 3. Based on the results, the escape latency to find a platform of normal control, asiatic acid, madecassic acid and madecassoside were rapidly dwindling after day 1 (Fig. 3A, 3C, 3D, and 3E). These four groups also exhibited a marked reduction in escape latencies as compared to trial 1 and trial 2 and reached stable latencies within 13 to 35s in four days. By contrast, the scopolamine group did not significantly change during the 4 days training session (Fig 3B). The asiatic group detected the lowest escape latencies (14s) as compared to saline group on day four (Fig. 3C). The escape latencies were shortened until four days except for scopolamine group exhibiting the formation of reference memory in rats.
Figure 3. Effects of saline (A), scopolamine (B), asiatic acid(C), madecassic acid(D), and madecassoside (E) on Morris water maze in Spraque-Dawley rats. Rats were given two sessions of trial each day for 4 consecutive days. The swimming time required for the rats to escape to platform was recorded in each day. Each group comprised a maximum of seven rat. The * indicated significant value with P < 0.05 and the ** indicate the significant value compared with saline (day 4). Ordinate shows the mean ± SEM
In this experiment, the effect of acute administration of three triterpene compounds as well as saline and scopolamine were investigated in male Spraque-Dawley rats. The results of locomotor activity experiment showed that asiatic acid, madecassic acid and madecassoside can enhance learning and memory behavioral performance via Morris water maze and this study confirmed that the increase in performance was not due to the influences of the motor activity in locomotor activity. The results of the locomotor activity indicate that asiatic acid, madecassic acid and madecassoside administrations did not alter the locomotor activity as compared with the saline group.
On the other hand, scopolamine administrations induced hyperactivity of locomotor in rats parallel with previous study done by , who demonstrated that scopolamine dependently induced hyperactivity locomotor in rats. Another triterpene group, asiaticoside which is of almost similar structure did not alter the total locomotor activity of the animals . Furthermore, an aqueous extract of C. asiatica (100-300 mg/kg) did not alter spontaneous locomotor activity in these rats thus excluding the possibility that the CNS depressant/stimulant activity of the herb had contributed to the changes in the passive avoidance and elevated plus maze tests .
The results of Morris water maze experiment showed that asiatic acid, madecassic acid and madecassoside improved the spatial memory similar to the saline group. The asiatic acid had the most prominent effect on spatial memory which can be translated as having significantly lowest escape latency compared to saline in day 4. Our previous data showed that asiatic acid acts as AChE inhibitor properties . This is parallel with previous study that the drugs that increased cholinergic levels facilitated behavioral performance in some rodent models of cognitive impairment [26-28].
The ACh-GABAergic feedback mechanisms were also affected by AChE inhibitor; physostigmine via increasing behavioral performance in rats. When the compound is activated, ACh is released via pyramidal neuron, thus the hippocampus GABAergic interneurons were also activated and sent the feedback to the medial septal region which is located at the basal ganglia that project cholinergic axons . Furthermore, increasing the available amount of ACh in hippocampus, by preventing its breakdown via physostigmine, eliminates the behavioral impairment observed after Pyrithiamine-induced thiamine deficiency (PTD) treatment. However, in normal rats, excessive activation of cholinergic activity can become dysfunctional [30,31].
According to  the standardized C. asiatica extracts significantly improved behavioural alterations in Morris water maze and attenuated increased AChE in mice. On the other hand, C. asiatica leaf extract has been demonstrated to improve spatial learning performance in neonatal rats during growth spurt period and increased the hippocampal cornu ammonis area 3 neuronal dendritic arborisation in rats [33,34]. According to  the ethanol extracts of C. asiatica leaf increases memory performance in rats after chronic stress. ACh levels played an excitatory role in the hippocampus area. Beyond exciting hippocampal pyramidal neurons, pharmacological activation of hippocampal muscarinic ACh receptors also directly excites GABAergic interneurons [29,36]. For the positive control, scopolamine groups showed impairment in Morris water maze and this is parallel with the previous study  which showed that scopolamine induced amnesic syndrome in a rat.
Asiatic acid administration is effective in enhancing spatial memory as well as with madecassic acid and madecassoside with no sedative effect on locomotor activity. Hence, asiatic acid administration might be useful in slowing down age-related deficits in memory and learning. The rapid development in the field of animal models of learning and memory processes may hopefully lead to an improved understanding of pathophysiology of cognitive disorders, and finally permit a rationale designing of therapeutic strategies for distinct cognitive dysfunctions through drug discoveries.
The authors are grateful to the Faculty of Medicine and Health Science, UniSZA and Department of Chemistry, UMT staff for work facilities and environment. This study was supported by a RAGS grant from Ministry of Higher Education Malaysia (RAGS/2012/UNISZA/ST04).
- Francis PT, Palmer AM, Snape M, Wilcock GK, The cholinergic hypothesis of Alzheimer’s disease:a review of progress. Journal Neurology Neurosurgery Psychiatry, 1999. 66: p. 10.
- Davidson M, et al., Cholinergic strategies in the treatment of Alzheimer's disease. Acta Psychiatr Scand Suppl, 1991. 366: p. 47-51.
- Palmer AM. Pharmacotherapy for Alzheimer's disease: progress and prospects. Trends Pharmacol Sci, 2002. 23(9): p. 426-33.
- Stafford, G.I., et al., Review on plants with CNSeffects used in traditional South African medicine against mental diseases. Journal of ethnopharmacology, 2008. 119(3): p. 513-37.
- Veerendra Kumar, M.H. and Y.K. Gupta, Effect of different extracts of Centella asiatica on cognition and markers of oxidative stress in rats. Journal of ethnopharmacology, 2002. 79(2): p. 253-60.
- Singh, B. and R.P. Rastogi, A reinvestigation of the triterpenes of Centella asiatica. Phytochemistry, 1969. 8(5): p. 917-921.
- Nasir, M.N., et al., Effects of asiatic acid on passive and active avoidance task in male Spraque-Dawley rats. Journal of ethnopharmacology, 2011. 134(2): p. 203-9.
- Luo, Y., et al., Neuroprotective effects of madecassoside against focal cerebral ischemia reperfusion injury in rats. Brain research, 2014. 1565: p. 37-47.
- Schimidt, H.L., et al., Memory deficits and oxidative stress in cerebral ischemia-reperfusion: neuroprotective role of physical exercise and green tea supplementation. Neurobiol Learn Mem, 2014. 114: p. 242-50.
- Guilherme dos Santos, J., Jr., et al., Cognitive enhancement in aged rats after chronic administration of Equisetum arvense L. with demonstrated antioxidant properties in vitro. Pharmacology, biochemistry, and behavior, 2005. 81(3): p. 593-600.
- Maulidiani, et al., Comparison of Partial Least Squares and Artificial Neural Network for the prediction of antioxidant activity in extract of Pegaga (Centella) varieties from 1H Nuclear Magnetic Resonance spectroscopy. Food Research International, 2013. 54(1): p. 852- 860.
- Jayashree, G., et al., Anti-oxidant activity of Centella asiatica on lymphoma-bearing mice. Fitoterapia, 2003. 74(5): p. 431-434.
- Djoukeng, J.D., et al., Antibacterial triterpenes from Syzygium guineense (Myrtaceae). Journal of Ethnopharmacology, 2005. 101(1–3): p. 283-286.
- Wolf, O.T. and C. Kirschbaum, Endogenous estradiol and testosterone levels are associated with cognitive performance in older women and men. Horm Behav, 2002. 41(3): p. 259-66.
- Chauhan, P.K. and V. Singh, Acute and Subacute Toxicity study of the Acetone Leaf extract of Centella asiatica in Experimental Animal Models. Asian Pacific Journal of Tropical Biomedicine, 2012. 2(2, Supplement): p. S511-S513.
- Nasir, M.N., et al., Inhibitory effect of asiatic acid on acetylcholinesterase, excitatory post synaptic potential and locomotor activity. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2012. 19(3-4): p. 311-6.
- Liang, J.-H., et al., The GABA(B) receptor allosteric modulator CGP7930, like baclofen, reduces operant self-administration of ethanol in alcohol-preferring rats. Neuropharmacology, 2006. 50(5): p. 632-9.
- Pitsikas, N., et al., The GABAB receptor and recognition memory: possible modulation of its behavioral effects by the nitrergic system. Neuroscience, 2003. 118(4): p. 1121-7.
- Wijeweera, P., et al., Evaluation of anxiolytic properties of Gotukola – (Centella asiatica) extracts and asiaticoside in rat behavioral models. Phytomedicine, 2006. 13(9–10): p. 668-676.
- Hosseini, M., et al., Chronic Treatment by L-NAME differently Affects Morris Water Maze Tasks in Ovariectomized and Naïve Female Rats. Basic and Clinical Neuroscience, 2011. 2(4): p. 47-52.
- Jeong, E.J., et al., KD-501, a standardized extract of Scrophularia buergeriana has both cognitive-enhancing and antioxidant activities in mice given scopolamine. Journal of ethnopharmacology, 2009. 121(1): p. 98- 105.
- Yu, Q.L., Hong, Q., Yoshihisa, T., Wen, Y. G., , A Novel Triterpene from Centella asiatica. Molecules, 2006. 11: p. 5.
- Sumanth, M., Sowmya, H., Nagaraj, S.V., Narasimharaju, K., , Efficacy of donepezil and galantamine in retrograde amnesia. Asian Journal of Pharmaceutical and Clinical Research, 2010. 3(4): p. 3.
- Chen, S.W., et al., Anxiolytic-like effect of asiaticoside in mice. Pharmacology Biochemistry and Behavior, 2006. 85(2): p. 339-344.
- Nasir, M.N., Habsah, M., Zamzuri, I., Rammes, G., Hasnan, J., Abdullah, J.,, Inhibitory effect of asiatic acid on excitatory synaptic transmission in the rat hippocampus. Current Neurobiology, 2010. 1(2): p. 8.
- Barnes, C.A., et al., Chronic treatment of old rats with donepezil or galantamine: effects on memory, hippocampal plasticity and nicotinic receptors. Neuroscience, 2000. 99(1): p. 17-23.
- Chang, Q. and P.E. Gold, Impaired and spared cholinergic functions in the hippocampus after lesions of the medial septum/vertical limb of the diagonal band with 192 IgG-saporin. Hippocampus, 2004. 14(2): p. 170-9.
- Mulder, J., et al., Galantamine-induced behavioral recovery after sublethal excitotoxic lesions to the rat medial septum. Behavioural brain research, 2005. 163(1): p. 33-41.
- Wu, M., et al., Acetylcholinesterase inhibitors activate septohippocampal GABAergic neurons via muscarinic but not nicotinic receptors. J Pharmacol Exp Ther, 2003. 307(2): p. 535-43.
- Bunce, J.G., H.R. Sabolek, and J.J. Chrobak, Intraseptal infusion of the cholinergic agonist carbachol impairs delayed-non-match-to-sample radial arm maze performance in the rat. Hippocampus, 2004. 14(4): p. 450-9.
- Elvander, E., et al., Intraseptal muscarinic ligands and galanin: influence on hippocampal acetylcholine and cognition. Neuroscience, 2004. 126(3): p. 541-57.
- Kumar, A., A. Prakash, and S. Dogra, Centella asiatica Attenuates D-Galactose-Induced Cognitive Impairment, Oxidative and Mitochondrial Dysfunction in Mice. Int J Alzheimers Dis, 2011. 2011: p. 347569.
- Mohandas Rao, K.G., S. Muddanna Rao, and S. Gurumadhva Rao, Enhancement of Amygdaloid Neuronal Dendritic Arborization by Fresh Leaf Juice of Centella asiatica (Linn) During Growth Spurt Period in Rats. Evid Based Complement Alternat Med, 2009. 6(2): p. 203-10.
- Rao, K.G.M., Rao S.M., Rao S.G.,, Centella asiatica (linn) induced behavioural changes during growth spurt period in neonatal rats. Neuroanatomy, 2005. 4: p. 5.
- Dwi Cahyani, R.S., Soedjono, A., Rina, S., Mawaddah, Ar-R., Djoko, P., Mansyur, R., Untung, T., Nur, A., , Ethanol Extracts of Centella asiatica Leaf Improves Memory Performance in Rats after Chronic Stress via Reducing Nitric Oxide and Increasing Brain-Derived Neurotrophic Factor (BDNF) Concentration. GSTF International Journal of Psychology, 2014. 1(1): p. 6.
- Widmer, H., et al., Evoked slow muscarinic acetylcholinergic synaptic potentials in rat hippocampal interneurons. Hippocampus, 2006. 16(7): p. 617-28.
- Oh, J.H., et al., Nelumbo nucifera semen extract improves memory in rats with scopolamine-induced amnesia through the induction of choline acetyltransferase expression. Neuroscience Letters, 2009. 461(1): p. 41- 44.