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The effect and its mechanism of Morinda officinalis how polysaccharide on chondrocyte apoptosis induced by IL-1β

Objective: To evaluate the effect and its mechanism of Morinda officinalis how polysaccharide on chondrocyte catabolism and apoptosis induced by IL (Interleukin)-1β.

Materials and Methods: Chondrocytes were randomly divided into 5 groups, control group, IL-1 beta group (50 μg/ml IL-1β in chondrocyte for 24 h), Morinda officinalis how polysaccharide group (2 μg/ml, 5 μg/ml and 10 μg/ml Morinda officinalis how polysaccharide adding to the chondrocyte). The apoptosis rate of the five groups was calculated and the relative expression of Bcl-2 (B Cell Leukemia 2), Bax (Bcl-2 Associated X Protein) and Caspase 9 were quantity by PCR and Western-blot. Elisa kit was used to measure the expression of iNOS in each group.

Results: 50 μg/ml IL-1β can significantly increasing the iNOS and apoptosis rates compared to the control group, and the difference was statistically significant (p<0.05), 10 μg/ml Morinda officinalis how polysaccharide can significantly reduce the chondrocytes apoptosis and iNOS level, the difference was statistically significant (p<0.05). The gene expression and protein expression of Bax and Caspase 9 in IL-1β group was higher than control group, and the Bcl-2 is lower than control group, the differences were statistically significant (p<0.05). Morinda officinalis how polysaccharide in 10 μg/ml can significantly reduce the gene expression and protein expression of Bax and Caspase 9 and down the expression of Bcl-2, the differences were statistically significant (p<0.05).

Conclusion: Morinda officinalis how polysaccharide can increase the expression of Bcl-2, reduce the expression of Bax and Caspase 9, thus can prevent chondrocytes apoptosis induced by IL-1β.

Author(s): Zhang Ru-guo, Huang Yong-hong, Zhang Jian-lin