Biomedical Research

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Research Article - Biomedical Research (2017) Volume 28, Issue 13

The effect and its mechanism of Morinda officinalis how polysaccharide on chondrocyte apoptosis induced by IL-1β

Objective: To evaluate the effect and its mechanism of Morinda officinalis how polysaccharide on chondrocyte catabolism and apoptosis induced by IL (Interleukin)-1β.

Materials and Methods: Chondrocytes were randomly divided into 5 groups, control group, IL-1 beta group (50 μg/ml IL-1β in chondrocyte for 24 h), Morinda officinalis how polysaccharide group (2 μg/ml, 5 μg/ml and 10 μg/ml Morinda officinalis how polysaccharide adding to the chondrocyte). The apoptosis rate of the five groups was calculated and the relative expression of Bcl-2 (B Cell Leukemia 2), Bax (Bcl-2 Associated X Protein) and Caspase 9 were quantity by PCR and Western-blot. Elisa kit was used to measure the expression of iNOS in each group.

Results: 50 μg/ml IL-1β can significantly increasing the iNOS and apoptosis rates compared to the control group, and the difference was statistically significant (p<0.05), 10 μg/ml Morinda officinalis how polysaccharide can significantly reduce the chondrocytes apoptosis and iNOS level, the difference was statistically significant (p<0.05). The gene expression and protein expression of Bax and Caspase 9 in IL-1β group was higher than control group, and the Bcl-2 is lower than control group, the differences were statistically significant (p<0.05). Morinda officinalis how polysaccharide in 10 μg/ml can significantly reduce the gene expression and protein expression of Bax and Caspase 9 and down the expression of Bcl-2, the differences were statistically significant (p<0.05).

Conclusion: Morinda officinalis how polysaccharide can increase the expression of Bcl-2, reduce the expression of Bax and Caspase 9, thus can prevent chondrocytes apoptosis induced by IL-1β.

Author(s): Zhang Ru-guo, Huang Yong-hong, Zhang Jian-lin

Abstract Full Text PDF