New pyrazoline derivatives were synthesized via the reaction of chalcones with cyclohexyl/phenyl thiosemicarbazide in presence of ethanol and acetic acid. All the compounds were confirmed by FT IR, 1H NMR, 13C NMR and MS spectral data. The compounds (1-11) were evaluated for antifungal activity against various strains of Candida species and compared with standard drug Itraconazole. MIC50 values were found to be within the range of 41.099-127.895 μg/ml and MIC90 values were found to be within the range of 62.121-240.955 μg/ml. Compound 7 was found to be most potent antifungal agent against Candida strains. It appears that para-methoxy substitution at one phenyl ring and meta-methoxy substitution at other phenyl ring of pyrazoline moiety made a significant contribution to the anti- Candidal activity in this series of pyrazolines.