In our country, the consumption demand of the kiwifruit has increased year by year. Every year, we need to import a large amount of kiwifruit from abroad to meet the domestic consumption demand. In order to improve the growth of kiwifruit, promote its good growth and development, and improve the yield, study on the effect of beneficial microorganisms on the growth of kiwifruit has a very important significance. By setting up the control analysis experiment, the experimental group and the control group were compared and analyzed, in order to explore the specific effects of beneficial microorganisms on the growth of kiwifruit. The experimental group was added with nitrogen fixing bacteria, phosphate dissolving bacteria and potassium bacteria. In addition, the growth environment of the control group and the experimental group was the same, and the growth of the plant of kiwifruit was determined. The culture was developed over a period of 90 days. Through the comparative analysis, it can be seen that the growth and development of the experimental group with beneficial microorganisms in the soil was better than that of the control group, and the disease resistance was better, which could absorb the elements such as Nitrogen (N), Phosphorous (P), Potassium (K) and so on. It can be concluded that the beneficial microorganisms in optimized soil can contribute to the growth and development of kiwifruit, and it can be used in the production practice.