Biomedical Research

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Research Article - Biomedical Research (2018) Volume 29, Issue 3

Research on roles played by stromal cell-derived factor 1 and chemokine receptor 4 in diagnosis on proliferation, infiltration and metastasis of cervical cancer

Purpose: To understand the influence of Stromal Cell-Derived Factor 1 (SDF-1) and corresponding receptor CXCR4 on proliferation, infiltration and metastasis of cervical cancer HeLa cells.

Method: The flow cytometry dyeing and the Western blot method are adopted to detect the expression conditions of CXCR4 in HeLa cells; the MTT method is adopted to detect the influence of SDF-1 and corresponding receptor CXCR4 on proliferation of HeLa cells; the Boyden chamber method is adopted to detect the influence of SDF-1 and corresponding receptor CXCR4 on infiltration and metastasis of HeLa cells.

Results: There is high expression on receptor CXCR4, the receptor of SDF-1; after the SDF-1 stimulation, the proliferation ability of HeLa cells is significantly increased (P<0.05), and this effect is dose-dependent; after the knock-down of CXCR4 expression on surface of HeLa cells with RNAi, the proliferation of HeLa cells caused by SDF-1 stimulation is significantly interdicted (P<0.05). SDF-1 may give rise to infiltration and metastasis of HeLa cells. After the knock-down of CXCR4 expression on surface of HeLa cells with RNAi, the infiltration and metastasis of HeLa cells are significantly interdicted (P<0.05).

Conclusion: The CXCR4 with high expression on surface of HeLa cells plays a significant role in inducing proliferation, infiltration and metastasis of HeLa cells.

Author(s): Feng Feng Cheng, Yan Kong, Aiju Fang, Jing Yu, Xiao Ying Zhang, Yingjie Li

Abstract Full Text PDF