The present study is to establish a rat model of abnormal phlegmatic syndrome with Erectile Dysfunction (ED) according to the theory of Uygur traditional medicine. In addition, the feasibility and reliability of this model is evaluated. A total of 100 mature male Sprague-Dawley rats were divided into 2 groups: normal control group (n=10) that had normal environment and diet, and model group (n=90) that was treated with spinach and coriander diet in a cold-wet environment. Biological parameters and sexual behavior of the rats were observed for 20 weeks until establishment of abnormal phlegmatic syndrome and ED model. Through erection test, the rats were randomly divided into 6 groups and disproved for 2 weeks. The levels of testosterone, luteinizing hormones, follicle stimulating hormone, estradiol and prolactin in the serum of the rats were measured using enzyme-linked immunosorbent assay. The abnormal phlegmatic syndrome and ED model rats showed inactivity, dull hair, clear urine, loose and soft stool, dark tongue, greasy tongue coating, decreased body weight and water consumption, and increased food consumption, urine and stool production. For sexual behaviors, increased mounting, erectile, insertion, and ejaculation latency and decreased mounting, erectile, insertion, and ejaculation numbers were observed in ED model rats. After 2 weeks of disproof treatment, biological parameters, sexual behavior and sexual hormones were reversed. The present study demonstrates that the model mimics the development of abnormal phlegmatic syndrome with ED and shows Biological characterization consistent with clinic patients. Moreover, the model can be reversed after treatment.