Biomedical Research

Research Article - Biomedical Research (2017) Volume 28, Issue 21

Preparation, characterization and in vitro antitumor effect of cholesterol succinyl Bletilla striata polysaccharide-loaded paclitaxel nanoparticles

Bletilla striata polysaccharide is a natural polysaccharide extracted from the dry tuber of Bletilla striata (Thunb.) Reichb. f. It is a type of natural hydrogel also known as Bletilla gum mainly comprising β-1, 4- mannose and β-1, 4-glucose. It is hydrophilic with the structure of rich active hydroxyl groups. After the introduction of hydrophobic cholesterol succinyl, it becomes a modified polysaccharide (CHSB) with amphipathy. Then the drug-loaded nanoparticle is prepared through dialysis with Paclitaxel (PTX) as the model drug, and its form is observed using Transmission Electron Microscopy (TEM). Its particle size, particle size distribution and zeta potential are tested with a Dynamic Light Scattering instrument (DLS). The entrapment rate and drug loading rate are determined by High Performance Liquid Chromatography (HPLC), and it’s in vitro release condition is investigated to establish the relationship between the entrapment rate and drug loading capacity, in vitro release and the substitution degree of modified polysaccharide cholesteryl. Paclitaxel (PTX), modified polysaccharide (CHSB), blank nanoparticle and drug-loaded nanoparticles are characterized by the differential scanning calorimetry method (DSC) and X-ray Diffraction method (XRD) so as to confirm the existing form of drugs in the drug-loaded nanoparticles. Finally, the in vitro antitumor activity and cell uptake of nanometer particles is investigated.

Author(s): Zhao Wencui, Zhang Qi, Wang Ying, Hou Jihua

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