One of the outstanding goals in nanotechnology, is produce the nanoparticles with the ability to control and reduce costs and environmental pollution. Recently, nanoparticles produced using living biological systems to create a new branch of nanotechnology. Silver nanoparticles are one of the most widely used nanoparticles. This study aimed to produce silver nanoparticles with Matricaria chamomilla and evaluation antibacterial and antifungal effects was performed, first Matric aria chamomilla extract were prepared by boiling, then in the vicinity of the extract with 20mM silver nitrate salt, reaction in the dark and at ambient tempura was carried out. Physicochemical properties of nanoparticles produced using a spectrophotometer and SEM device was studied. Eventually, the antibacterial and antifungal effects of these nanoparticles was evaluated. After study of the spectrum of UV-Vis and SEM showed that Matricaria chamomilla capable of producing silver nanoparticles extracellular with rang of 60 to 65nm and with is different morphology. It is clear that the nanoparticles have good antibacterial and antifungal properties against bacteria and yeast that used in this study. Matricaria chamomilla is a reducing agent suitable and compatible with environment for produce nanoparticles.