Biomedical Research

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Research Article - Biomedical Research (2017) Volume 28, Issue 8

Metabolomics analysis of human pancreatic cancer tissue and paired adjacent tissue samples

Background/aims: This study aimed to apply 1H spectrum Nuclear Magnetic Resonance spectroscopy (1H-NMR) to detect the metabolites in pancreatic cancer tissue and paired adjacent tissue samples.

Methods: 48 samples were taken from cancer tissues of 24 pancreatic cancer patients and paired adjacent tissues. The samples were subjected to 1H-NMR and principle component analysis to compare endogenous small-molecule metabolites.

Results: Leucine and isoleucine levels were significantly higher in pancreatic cancer tissues than in control tissues. Lactate, betaine, choline, α-glucose and α-hydroxybutyrate levels were significantly lower in pancreatitic cancer tissues than in control tissues.

Conclusions: 1H-NMR-based metabolomics is an effective method to identify small-molecule metabolites in pancreatic cancer tissues. These metabolites can be used as the metabolic markers of pancreatic cancer for early diagnosis.

Author(s): Dong Ouyang, Qingyue Dai

Abstract Full Text PDF