Novel influenza A/H1N1 virus has a pandemic outbreak all around the world and makes a serious concern for global health organ systems. While, elevation in liver function tests is observed in associate with contaminating with influenza A/H1N1. The study was aimed to evaluate the liver function tests abnormality in Influenza H1N1 in south-eastern of Iran. 330 patients who referred to Ali-ibn Abi Talib Hospital, Zahedan University of Medical Science, Iran with the primary symptoms of Influenza A/H1N1 were participated. All hospitalized patients were subjected for nasopharyngeal RT-PCR for pandemic H1N1 virus. Also, liver function tests including AST (Aspartate Aminotransferase), ALP (Alkaline Phosphatase), ALT (Alanine Transaminase), PT (Prothrombin Time), PTT (Partial Thromboplastin Time), TBIL (Total Bilirubin) and DBIL (Direct Bilirubin) were done for patients. At the end statistical analysis was performed in order to compare the results from both RT-PCR test and liver tests. Spss version 20 was used to analyse the data and data were evaluated by chi-square test. 150 persons were positive for H1N1 by RT-PCR. The values of AST, ALP, PT and PTT were increased statistically but TBIL and DBIL showed no significant difference. In conclusion, we forcefully supposed a close relationship between liver function and immune system elements.