The aim of this study was to prescreen the expression of ALK-positive in signet-ring cell gastric carcinoma by IHC assay. We selected 84 FFPE samples with signet-ring cell gastric carcinoma (55 cases of GC, 29 cases of EGJ) and performed the detection of ALK-positive using IHC. The correlation of ALK-positive and clinicopathologic characteristics was statistically analyzed. The results showed, of 84 cases prescreened, 11 (13.09%) were ALK-positive. For the 6 cases with IHC 2+ (7.14%), and the 5 cases with IHC 1+ (5.95%). We noted that 8 (72.73%) cases were never smoker, 8 (72.73%) cases were >5 cm tumor size and 9 (81.82%) cases were T4 in invasive depth. All of the 11 cases were III of pathologic TNM stage. The ALK-positive patients showed significantly statistical difference in lymph node metastasis (p=0.0285) and TNM stage (p=0.0497), compared with the ALK-negative patients. In conclusion, the expression of ALK fusion is found in signet-ring cell gastric carcinoma by IHC assay.