Biomedical Research

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Identification and analysis of toll like receptor 4 (TLR4) level changes in vascular dementia patients related type 2 diabetes mellitus

Objective: Since the Vascular Dementia (VD) is one of serious complication of Type 2 Diabetes Mellitus (T2DM), we study the molecular mechanism of Toll Like Receptor 4 (TLR4) in autoimmunity respond on VD patients.

Methods: The clinical symptoms of VD related T2DM patients are investigated and VD risk factors are analysed. We exam the blood samples by immunofluorescence assay. After collecting the patients’ Cerebrospinal Fluid (CSF), it tests the level of TLR4 in CSF and blood at gene and protein degrees, respectively. Flow cytometry is used to verify the higher level of TLR4 play an important role in the autoimmune response of VD patients.

Results: The average incidence of T2DM is 1.36%, and VD incidence is 0.33% in 2016. The VD patients are concentrated in old and middle-age women. The risk factors of VD are including rural residence, inadequate health-management, >7 years of T2DM-history and mental pressure. TLR4 protein were at higher presence in VD groups than the normal T2DM group and control group, which consistent with the results of TLR4 mRNA expression in CSF. Additional, their CD4+/CD8+ ratio is increased significantly, associated with TLR4 increasing expression.

Conclusion: VD is directly related with TLR4 increasing expression, which is a threat to the health of the T2DM patients.

Author(s): Fan Yaochun, Ou Xia, Wang Wenrui, Tian Xiaoling, Yan Shaohong, Hu Nan, Zhang Xiaoyan, Xing Wanjin