In this study, the clinical transfusion effects of fresh and cryopreserved apheresis leukocyte-reduced Platelets (PLTs) were examined. In total, 102 patients admitted to our hospital between August 2012 and August 2013 were enrolled, including 50 non-hematonosis patients and 52 hematonosis patients. Among them, 45 received fresh apheresis PLTs and 57 received cryopreserved PLTs. Patient blood PLT counts before and after transfusion were measured and the Percentage of Platelet Recovery (PPR) was compared among groups. The PPR for the cryopreserved PLT group was 25.03 ± 52.06%, which was significantly lower than that of the fresh PLT group (47.06 ± 42.94%, t=2.29, P<0.05). The PPR of the non-hematonosis group (51.01 ± 65.10%) was significantly lower than that of the hematonosis group (21.40 ± 24.23%; t’=2.92, P<0.05). Fresh apheresis leukocyte-reduced PLTs were safer and more effective than cryopreserved apheresis leukocyte-reduced PLTs. Transfusion was more effective in nonhematonosis patients than in hematonosis patients.