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Clinical significance of miRNA-433 expression in hepatocellular carcinoma

Background: Previous studies found miRNAs can play important role in the pathogenesis, metastasis and prognosis of hepatocellular carcinoma by regulating the expression of its target genes. We evaluated the clinical significance of miRNA-433 expression in hepatocellular carcinoma.

Method: We collected 28 primary hepatocellular carcinoma specimens matched by 28 normal paracarcinoma tissues. The RNA concentration was calculated by the following formula: OD260 × 50 times × 40/100. We detected the absorbance at 260 nm and 280 nm, and calculated OD260/OD280. The purity was considered to be acceptable when the ratio value fallen into between 1.8 and 2.0. The expression of MiR-433 was analysed via Real time-PCR method. The Cq value was calculated, and Data were processed using 2-ΔΔCT method.

Results: According to the 2-ΔΔCT method, the expression of miR-433 in the hepatocellular carcinoma specimens was significantly lower than that in the normal tissue specimens (3.61 ± 1.35 vs. 9.39 ± 2.46, t=-10.899, P<0.001). The receiver operating characteristic cure was used to identify the diagnostic value of miR-433 expression levels in hepatocellular carcinoma. The area under curve of miR-433 was 0.770 (95% confidence interval: 0.594-0.947).

Conclusion: The low expression of miR-433 in hepatocellular carcinoma may be related to occurrence of hepatocellular carcinoma. miR-433 expression might have a moderately diagnostic value to hepatocellular carcinoma.

Author(s): Zhanzhan Li, Yanyan Li, Jing Xue