Biomedical Research

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Bakuchiol inhibits cell proliferation and induces apoptosis and cell cycle arrest in SGC-7901 human gastric cancer cells.

The main purpose of the present study was to evaluate the antiproliferative activity of Bakuchiol in human gastric tumor cell lines (SGC-7901) along with demonstrating its mode of action. The effect of this compound on cell viability was evaluated by MTT assay. Fluorescence and phase contrast microscopic techniques were used to study the effect of the compound on cellular morphology and apoptosis. Flow cytometry was used to assess the effect on cell cycle phase distribution. Results revealed that Bakuchiol exerted potent, dose-dependent as well as time-dependent growth inhibitory effects in SGC-7901 cell proliferation with IC50 values of 58.4, 42.3 and 32.5 μM at 12, 24 and 48 h time intervals respectively. On treatment with 10, 50 and 100 μM dose of bakuchiol for 48 h, phase contrast microscope revealed that the cells got detached from one another making clusters of small number of cells floating in the medium. After the cells were treated with 10, 50 and 100 μM concentrations of bakuchiol, cells began to emit orange red fluorescence more heavily at the centre of cells indicating apoptosis. Bakuchiol also induced sub-G1 cell cycle arrest in a dose-dependent manner. The current findings reveal that Bakuchiol is a potent cytotoxic agent against gastric cancer cells and its cytotoxicity is mediated through induction of apoptosis and sub-G1 cell cycle arrest.

Author(s): Jing Lin, Hui-Jing Yao, Rui-Yun Li