Objective: The aim of the present study was to measure, using Atomic Absorption Spectrometry (AAS), calcium ions released from three calcium silicate-based materials, white MTA (WMTA), Bioaggregate (BA), and Biodentine, after their immersion in 0.2% chitosan, 10% propolis, 1% acetic acid, and 17% EDTA and also in distilled water.
Methods: One hundred and fifty silicon tubes were prepared in this study. The tubes were randomly divided into three groups and filled as follows. Group I: MTA, Group II: BA Group III: Biodentine. After that, the set material was scraped from a silicon tube. Each group was then randomly divided into five subgroups of 10 samples each (n=10). For subgroup 1, a fresh 0.2% chitosan solution was prepared. For subgroup 2, 10% propolis solution was prepared. Subgroup 3 was treated with distilled water. For subgroup 4, 1% acetic acid was used. In subgroup 5, 17% EDTA was used. The samples were immersed in the test solutions for 10 minutes. AAS (GBC-GF; model Avanta PM) was used to determine the Ca ions released. The data were analysed using the SPSS 20 software. Kruskal-Wallis and Mann-Whitney U tests were applied (P<0.05).
Results: The lowest Ca release values were observed in distilled water, while the highest values were found in the samples incubated in the EDTA, and there are no statistically significant differences between propolis, chitosan, and distilled water.
Conclusions: Chitosan and propolis natural irrigation solutions may be preferred to EDTA, when used with MTA, BA, and Biodentine.