Biomedical Research

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Research Article - Biomedical Research (2017) Volume 28, Issue 2

Anticancer effects of Gardenia jasminoides in HepG2 human hepatoma cells

Background: Gardenia jasminoides is a natural plant, it has many biological activities. Its ability of cancer cell apoptosis needs to be researched.

Methods: In this study, the important biological activity materials were determined by high efficiency liquid chromatography. The in vitro anticancer effects were measured by MTT and RT-PCR assays in HepG2 human hepatoma cells.

Results: Gardenia jasminoides contained 1.03% genipin gentiobioside, 5.90% gardenoside, 1.26% crocin 1 and 0.17% crocin 2. Gardenia jasminoides had significantly strong HepG2 cells inhibitory effects, and highest effect 80.2% is showed in 400 μg/mL concentration of Gardenia jasminoides which is remarkable. the Gardenia jasminoides also have the ability to increase the mRNA expression of caspase-3, caspase-8, caspase-9, Bax, TRAIL, Fas, Fas/FasL, p53, p21, IκB-α and decrease the Bcl-2, BclxL, XIAP, cIAP-1, cIAP-2, survivin, NF-κB expression in HepG2 cells. The higher concentration of Gardenia jasminoides also showed the higher increasing or decreasing mRNA expression effects in HepG2 cells.

Conclusion: Gardenia jasminoides had a strong anticancer effect through its apoptosis inducing abilities in hepatic cancer; it could be used for the treatment as a medicine or health product in daily human life for good liver health.

Author(s): Shao-cheng Chen, Xin Zhao, Ruo-kun Yi, Jun Qian, Yan-hong Shi, Rui Wang

Abstract Full Text PDF