Klebsiella pneumoniae is one of the most important opportunistic enteric bacteria and is a major cause of pneumonia and urinary tract infection. Serotype capsules of K1 and K2 can cause intense diseases. Acquisition of plasmid that codes the production of ESBLs confers on K. pneumoniae resistance to number of broad spectrum antibiotics posing a global public health problem. Integron is one of the important factors of multi resistance in gram negative microorganism’s especially intestinal bacteria. The magA gene rmpA gene was studied in 90 isolates of K. pneumoniae from different clinical cases in Shahrekord city, Iran. The frequency of resistance genes qnr, sul 1, tetB, tetA and aac (3) IIa at the presence of specific primers were examined and all resistant isolates were tested for detection of sul1, sul2, sul 3 and int1 genes using special primers. Of the 90 isolates, 13 had serotype K1A with redundancy of 14.44% and 15 cases had serotype K2A with the redundancy of 16.66%. rmpA gene was observed in 10 isolates the redundancy of 11.11%. In this study 33 isolates resistance to cotrimoxazole, aren’t finding sul1 gene in 15 isolated cases, sul2 gene in 20 isolated cases, sul3 gene in 2 isolated cases, respectively. Also there were 27 demonstrating int1 genes for Cotrimoxazol. The study has revealed that serotype K1 is one of the most important serotypes of K. pneumonia. Also there seems to be a strong relationship between presence of Integron and increased resistance to different antibiotics.