As specific stem cells of skin tissue, epidermal stem cells (ESCs) have been proved to be closely related to the wound repair. As a result, studying more targeted markers for a rapid acquisition of ESCs has become a hot issue for TES. For server burn wound or ulcer, the creation of ideal scaffold as matrix for cell proliferation and differentiation was needed for TES as useful ESCs being rare. In this review, the common use of the markers for the isolation and identification of ESCs are summarized. Furthermore, the research and development of tissue engineering skin scaffold during the past years were also discussed.