mass-spectrometry-congress-2019-scientifictracks

6 / 19

Note:
Page 26
May 20-21, 2019 | Rome, Italy
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
YEARS
Journal of Chemical Technology and Applications | Volume 3
MASS SPECTROMETRY,
PROTEOMICS AND POLYMER CHEMISTRY
3rd International Conference on
Mass Spectrometry Congress 2019
CHEMICAL TOOLS FOR SELECTIVE DETECTION OF MONOMETHYL LYSINE PTMS
Monika R 1 and Yonnette Sim2
1 Auburn University, USA
2 Seton Hall University, United States
S elective modification of biomolecules provides scientists with an effective tool for a multitude of bio analyt-
ical, therapeutic, biological and bioengineering applications. However, chemical strategies that can target
a particular functional group at a single site in the presence of reactive amino acid side chains on protein
surfaces are limited. We have developed a multicomponent bioconjugation approach for selective labelling of
proteins containing secondary amines. This method does not require any genetic engineering of the protein
target and protection of the side chains of other amino acids. The resulting bioconjugation reaction leads to
the formation of a highly stable C-C bond at the site of the conjugation. The broad utility of the bioconjugation
reaction is demonstrated by conjugation of various probes such as dye, peptides and PEG on different proteins
containing a proline at the N-terminus such as creatine kinase and aldolase. This method is employed for label-
ling monomethyl lysine containing post-translational modifications (PTMs) on proteins with various cargoes.
The dysregulation of monomethyl lysine PTMs has been linked to a variety of different biological malfunctions,
yet the chemical methods for selective detection of monomethyl lysine PTMs are still lacking. This selective
tagging methodology can effectively detect monomethyl lysine PTMs thus has a potential to further our un-
derstanding of the role of monomethylated lysine containing PTMs in regulating various cellular signalling
processes.
Monika R et al., J Chem Tech App 2019, Volume 3
Monika R has completed her PhD in 2009 from Indian Institute of Technology, Kanpur, India and she completed her Postdoctoral
from New York University, USA. Currently she is working as a Professor at Auburn University, USA.
BIOGRAPHY