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Page 23
May 20-21, 2019 | Rome, Italy
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
YEARS
Journal of Chemical Technology and Applications | Volume 3
MASS SPECTROMETRY,
PROTEOMICS AND POLYMER CHEMISTRY
3rd International Conference on
Mass Spectrometry Congress 2019
SWATH MASS SPECTROMETRY AS A TOOL FOR QUANTITATIVE PROFILING OF THE
BONE MARROW PLASMA FROM ALCOHOLIC LIVER DISEASE
Renu Goel, Dhananjay Kumar, Ankur Kumar and Anupam kumar
Translational Health Science and Technology Institute, India
Alcoholic hepatitis is characterized by acute or acute-on-chronic hepatic failure and associated with a high
mortality. Specific therapies should be considered for those at high risk of mortality. Model for End-Stage
Liver Disease (MELD) score is a marker of disease severity and mortality in persons with chronic alcoholic liver
disease. Author’s aim is to find out a diagnostic biomarker for disease severity along with the MELD score which
can be used as a predictor of short termmortality in persons with alcoholic hepatitis. Understanding molecular
pathogenesis is pivotal in managing the disease. They employed sequential window acquisition of all theoret-
ical mass spectra (SWATH-MS) to seek crucial proteins involved in disease progression. Bone marrow plasma is
taken from chronic liver disease patient as a part of stem cells therapy clinical trial. In this study, a quantitative
proteomic of bone marrow plasma with low and high MELD scores were compared with normal bone marrow
plasma from non-cirrhotic portal hypertension patient whose liver function test was normal using a SWATH-
MS strategy. In total, 232 proteins were differentially expressed in all groups. 17 proteins are down regulated
and 81 up regulated in patients with MELD score <15 with control. Moreover, 37 proteins are down regulated,
59 up regulated while comparison of MELD score >15 with control. Inhibition of coagulation, complement
and intrinsic prothrombin pathways are revealed by functional analysis. Humoral immune response, immune
cell trafficking and inflammation pathways are enriched under physiological system development. Proteins
preliminarily discovered in this study may be associated with dysregulation bone marrow microenvironment
during disease progression. To the author’s knowledge, this study presents the most complete view of bone
marrow plasma in low and high MELD score, identifying hundreds of differentially expressed proteins, which
together form a rich resource for novel drug targets or diagnostic biomarker discovery.
Renu Goel et al., J Chem Tech App 2019, Volume 3
Renu Goel has obtained her M.Tech and PhD degree in Biotechnology from Institute of Bioinformatics, India with Prof. Akhilesh Pan-
dey, Johns Hopkins University, Maryland. Her research group is working on identifying pathways or proteins involved in early stages
of progression of diseases such as type II diabetes, liver diseases and dengue fever by using proteomics, metabolomics and bioin-
formatics approaches. She has also worked on draft map of human proteome published in Nature. During this period she has pub-
lished around 30 research articles in high impact factor international journals. She is an Editorial Board Member for many journals.
BIOGRAPHY