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allied
academies
Journal of Microbiology: Current Research | Volume 2
November 01-02, 2018 | London, UK
7
th
European
Clinical Microbiology Congress
4
th
International Conference on
Ophthalmology and Eye Disorder
Joint Event
&
War on terror cells: Novel sources of antimicrobials
Naveed Ahmed Khan
Sunway University, Malaysia
W
iththeworseningtrendsofdrugresistance,thereisaneed
for newer and more powerful antimicrobial agents. The
search for new compounds originating from natural resources
is a promising research area. We hypothesized that animals
living in polluted environments are potential source of novel
antimicrobial molecules. Under polluted milieus, organisms
such as cockroaches encounter different types of microbes,
including superbugs. Such creatures survive the onslaught
of superbugs and are able to ward off disease by producing
antimicrobial substances. Here, we characterized antibacterial
properties in extracts of various body organs of cockroaches
(
Periplaneta americana
) and showed potent antibacterial
activity in crude brain extract against methicillin-resistant
Staphylococcus aureus
and neuropathogenic
E. coli
K1. The size-
exclusion spin columns revealed that the active compound(s)
are less than 10 kDa in molecular mass. Using cytotoxicity
assays, it was observed that pre-treatment of bacteria with
lysates inhibited bacteria-mediated host cell cytotoxicity. Using
spectra obtained with LC-MS on Agilent 1290 infinity liquid
chromatograph, coupled with an Agilent 6460 triple quadruple
mass spectrometer, tissues lysates were analyzed. Among
hundreds of compounds, only a few homologous compounds
were identified that contained isoquinoline group, chromene
derivatives, thiazine groups, imidazoles, pyrrole containing
analogs, sulfonamides, furanones, flavanones and known to
possess broad-spectrum antimicrobial properties and possess
anti-inflammatory, anti-tumour and analgesic properties.
Further identification, characterization and functional studies
using individual compounds can act as a breakthrough in
developing novel therapeutics against various pathogens
including superbugs.
e:
naveed5438@gmail.comClinical Microbiology and Eye 2018, Volume 2
DOI: 10.4066/2591-8036-C1-003