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academies
Journal of Microbiology: Current Research | Volume 2
November 01-02, 2018 | London, UK
7
th
European
Clinical Microbiology Congress
4
th
International Conference on
Ophthalmology and Eye Disorder
Joint Event
&
Genomic features, novel antibiotic resistance genes and pan-genome analysis of clinical multidrug-re-
sistant
Acinetobacter baumannii
strains in Vietnam hospital
Si-Tuan Nguyen
Thongnhat Dongnai General Hospital, Vietnam
Background:
Acinetobacter baumannii
is an important
nosocomial pathogen that can develop multidrug resistance.
In this study, we sought to explore the genomic properties,
phylogenetic relationships and comparative genomics of this
pathogen through strain DMS06669 and DMS06670 (isolated
from the sputum of two male patients with hospital-acquired
pneumonia).
Methods:
Whole genome analysis of
A. baumannii
DMS0669
and DMS06670 included
de novo
assembly; gene prediction;
functional annotation to public databases; phylogenetic tree
construction by average nucleotide identity; pan-genome
analysis and antibiotics resistance genes identification.
Antibiotics resistance genes
in-vitro
were isolated by PCR and
re-confirmed by improved Sanger method.
Results:
The data showed that a total of 19 possible antibiotic
resistance genes, conferring resistance to eight distinct classes
of antibiotics, were identified in two strains. Nine of these genes
have not previously been reported to occur in
A. baumannii
.
Comparative analysis of 23 available genomes of
A. baumannii
revealed an open pan-genome consisting of 15,883 genes. All
antibiotics resistance genes were isolated.
Conclusions:
Our results provide important information
regarding mechanisms that may contribute to antibiotic
resistance in the DMS06669 and DMS06670 strain and
have implications for treatment of patients infected with
A.
baumannii.
e:
nsituan@gmail.comClinical Microbiology and Eye 2018, Volume 2
DOI: 10.4066/2591-8036-C1-003