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Journal of Microbiology: Current Research | Volume 2
November 01-02, 2018 | London, UK
7
th
European
Clinical Microbiology Congress
4
th
International Conference on
Ophthalmology and Eye Disorder
Joint Event
&
Exposure to manuka honey modules antibiotic susceptibility on wound isolates
Jawahir Mokhtar, Andrew J McBain, Ruth G Ledder
and
Gavin Humphreys
The University of Manchester, UK
Background:
The clinical application of Manuka honey has
recently gainedmomentum, particularly in treatment of chronic
wound infections. Changes in antibiotic susceptibility have
been observed previously, following the exposure of bacteria to
subtherapeutic concentrations of honey, however such findings
have been limited to methicillin-resistant
Staphylococcus
aureus
,
Streptococcus pyogenes
and
Pseudomonas aeruginosa
.
The aim of this study is to assess the modulation of antibiotic
sensitivity in a broader panel of chronic wound isolates.
Methods:
Parent strains (P0) of
Staphylococcus aureus
, MRSA,
Staphylococcus. epidermidis
,
S. pyogenes
,
P. aeruginosa
,
Escherichia coli
,
Klebsiella pneumoniae
and
Proteus mirabilis
were passaged ten times in the presence of sub-lethal
concentrations of clinical grade Manuka honey to generate
strain P10. In order to assess any permanent or transient
changes in bacterial susceptibility, the bacteria were grown in
honey-free media for a further 10 passages (X10). Antibiotic
sensitivity testing was performed using a combination of
microdilution and disc diffusion methodologies.
Results:
Variable changes in bacterial susceptibilities were
noted following subtherapeutic exposure to honey. P10 strains
of
S. epidermidis
and
S. pyogenes
exhibited a ≥4-fold decrease
in their sensitivities to erythromycin and tetracycline in
comparison tobaseline values. Similarly,
E. coli
displayeda4-fold
reduction in susceptibilities to gentamicin following passaging
with honey. In contrast,
K. pneumoniae
and
P. mirabilis
showed
notable increases in susceptibility towards both ciprofloxacin
and gentamicin after 10 passages in the presence of honey. All
changes in MIC, MBC and MBEC were shown to be transient
in nature with the exception of
K. pneumoniae
and
P. mirabilis
(X10), which exhibited an MIC to ciprofloxacin >4 fold greater
than the parent strains.
Conclusion:
Wound isolates exposed to clinical grade Manuka
honey exhibited transient changes in antibiotic profiles. The
underlyingmechanismand clinical implications of such changes
are unclear and warrant further investigation.
Speaker Biography
Jawahir Mokhtar is currently pursuing her PhD at the University of Manchester, UK.
e:
jawahir.mokhtar@postgrad.manchester.ac.ukJawahir Mokhtar et al, Clinical Microbiology and Eye 2018, Volume 2
DOI: 10.4066/2591-8036-C1-003