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Cell and Gene Therapy 2018 & Clinical Microbiology Congress 2018 Biomedical Research | ISSN: 0976-1683 | Volume 29
S e p t e m b e r 1 0 - 1 1 , 2 0 1 8 | D u b l i n , I r e l a n d
alliedacademies Joint Event on
CLINICAL AND MEDICAL MICROBIOLOGY
CELL AND GENE THERAPY
&
World Congress on
International Conference on
Nahla E El-Ashmawy et al., Biomed Res 2018, Volume 29 | DOI: 10.4066/biomedicalresearch-C3-007
ENHANCED ANTICANCER EFFECT AND
REDUCED TOXICITY OF DOXORUBICIN IN
COMBINATION WITH THYMOQUINONE
RELEASED FROM POLY-N-ACETYL
GLUCOSAMINE NANOMATRIX IN MICE
BEARING SOLID EHRLISH CARCINOMA
Nahla E El-Ashmawy1, Eman G Khedr1, El-Zeiny M Ebeid1,
Mohamed L Salem1, Abdel-Aziz A Zidan2 and Esraa M Mosalam3
1Tanta University, Egypt
2Damanhur University, Egypt
3Menoufia University, Egypt
The incidence of breast cancer remarkably increases all over the world.
Therefore, there is a great demand to introduce new approaches into
cancer treatment field. The current study was designated to evaluate the role
of doxorubicin (DOX) and/or thymoquinone (TQ) nano matrix in potentiating
the cytotoxicity of either drug, and to investigate the ability of TQ to reduce
cardiotoxicity of DOX in solid Ehrlich carcinoma (SEC)-bearing mice. DOX
and TQ were loaded into F2 gel, which is a fully-acetylated poly-N-acetyl
glucosamine nanofiber. SECwas induced in female albinomice as amodel for
experimentally induced breast cancer. Mice were randomly divided into eight
groups (n=10): normal control, tumor control, F2 gel, free DOX, DOX+F2 gel,
free TQ, TQ +F2 gel, and DOX+ TQ+ F2 gel. On day 28th from tumor inoculation,
mice were sacrificed, and blood samples were collected for measurement of
the cardiac markers; lactate dehydrogenase (LDH) and creatine kinase (CK-
MB). In addition, cardiac tissuewas utilized for determination of lipid peroxide,
and tumor tissue was used for measurement of anti-apoptotic protein Bcl-2
as well as gene expression of the tumor suppressor gene P53. DOX and/or
TQ showed a significant reduction in tumor volume, cardiac markers, tumor
Bcl-2, and P53 upregulation compared to free conventional therapies. Co-
treatment with DOX+ TQ+ F2 gel was superior to all other groups in exerting
beneficial effects. Use of TQ as an adjuvant therapy with DOX could improve
its cytotoxic effects and limit its cardiac toxicity. Furthermore, loading of DOX
and/or TQ into F2 gel showed a remarkable anti-cancer activity.
Nahla E El-Ashmawy is the Dean of Faculty of Phar-
macy, Tanta University, Egypt.
BIOGRAPHY