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Cell and Gene Therapy 2018 & Clinical Microbiology Congress 2018 Biomedical Research | ISSN: 0976-1683 | Volume 29
S e p t e m b e r 1 0 - 1 1 , 2 0 1 8 | D u b l i n , I r e l a n d
alliedacademies Joint Event on
CLINICAL AND MEDICAL MICROBIOLOGY
CELL AND GENE THERAPY
&
World Congress on
International Conference on
Richard J DiPaolo et al., Biomed Res 2018, Volume 29 | DOI: 10.4066/biomedicalresearch-C3-007
DIAGNOSTIC ASSESSMENT OF
IMMUNOLOGICAL HISTORY BY HIGH-
THROUGHPUT TCR SEQUENCE ANALYSES
Richard J DiPaolo1, Kyle Wolf1, Tyler Hether2, Pavlo Gilchuk4,5,
Amrendra Kumar4,5, Julie Maybruck3, Mark Buller1 and Sebastian
Joyce4,5
1Saint Louis University, USA
2Adaptive Biotechnologies, USA
3Federal Bureau of Investigations, USA
4Tennessee Valley Healthcare System, USA
5Vanderbilt University, USA
During an immune response T-cells expressing unique T-cell receptor DNA
rearrangements undergo clonal expansion. We hypothesized these unique
TCR sequences can serve as diagnostic classifiers, providing information of
immunological exposures. We used high-throughput sequencing to identify
TCRβ sequences in separate cohorts of mice after smallpox vaccination and
after Monkeypox virus (MPXV) infection. From millions of sequences in the
peripheral blood we identified 315 TCR sequences associated with post-
vaccinated samples. Vaccine-associated TCR sequences (VATS) were used
as diagnostic classifiers and were used to correctly identify 100% of naive and
post-vaccinated samples. The VATS were also 98% accurate at identifying
samples from the independent cohort of samples infected with a highly
related MPXV. The reproducibility of this method was verified repeating the
analyses after identifying MPXV-associated TCRβ sequences (MATS). MATS
distinguished infected/vaccinated samples from naïve (98% accuracy). The
data show that computational identification of vaccine/pathogens expanded
TCRs is a sensitive and specific method for determining exposure and can be
used to track pathogen specific immune cells with unprecedented sensitivity.
Richard J DiPaolo is currently working as a Professor
in Molecular Microbiology and Immunology at Saint
Louis University, USA. He has pursued his Postdoc-
toral fellowship from NIAID, National Institutes of
Health, PhD from Washington University in Saint Lou-
is in 2002 and BA at University of Chicago in 1995.
His research interest is in establishing mouse models
of human diseases to develop strategies to suppress
chronic inflammatory diseases. He currently has an
on-going project to examine the T and B cell respons-
es to vaccine and infections and his goal in this project
is to understand which immune receptors are used to
recognize different vaccines and infectious agents.
He had several publications as well as he is a principal
investigator in American Gastroenterological Associa-
tion, American Cancer Society, Washington University,
and Arthritis National Research Foundation.
BIOGRAPHY