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Microbiology: Current Research 2017 | Volume 1, Issue 2
Joint Conference
GLOBAL APPLIED MICROBIOLOGY CONFERENCE
MICROBIAL & BIOCHEMICAL RESEARCH AND TECHNOLOGIES October 18-19, 2017
Toronto, Canada
International Congress on &
Large-scale molecular detection of microorganisms
Sona Pekova
Vemodia a.s., Laboratory for veterinary molecular diagnostics, Czech Republic
With the advent of high-throughput molecular
techniques inmolecularmicrobiology (NextGeneration
Sequencing, NGS) it became possible to exploremicrobiomes
of rather complicated niches, such as microbiome of the gut,
genitourinary tract, skin, or environmental microbiomes. The
NGS technology enables to decipher even the most complex
biological samples and analyze the DNA contents thereof.
We have developed a large-scale 16S rDNA (panbacterial)
and 18S rDNA (panfungal) NGS approach, combined with
quantitative Real-Time PCR, to identify the microbiome and
fungome in their complexities, in a quantitative way. This
technological approach allows us to assess the microbial
flora of a given anatomic location in full extent, with direct
relevance to the rational selection of antimicrobial agents,
if relevant. This high-throughput approach also allowed us
to identify novel microbial agents, previously not suspected
to infect humans – e.g. Candidatus Neoehrlichia mikurensis
in two immunocompromised individuals, or several zoonotic
Chlamydia and Mycoplasma species directly transmissible to
humans. The presented data will cover a unifying concept
of molecular microbiology, entailing both human and
veterinary infections, as the overlap between both is greater
than previously anticipated.
Speaker Biography
Sona Pekova has completed her graduation as MD from the Medical Faculty, Charles
University in Hradec Kralove, Czech Republic. She continued her PhD studies at the
Academy of Sciences of the Czech Republic in Prague and the 1st Medical Faculty,
Charles University in Prague, Czech Republic. She has her PhD in Molecular Biology,
Virology and Immunology. She has extensive expertise in molecular hematology,
molecular hematooncology, molecular microbiology and molecular genetics, both
in human and veterinary medicine, with tens of peer reviewed scientific papers
documenting her medical and scientific history.
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