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Microbiology: Current Research 2017 | Volume 1, Issue 2
Joint Conference
GLOBAL APPLIED MICROBIOLOGY CONFERENCE
MICROBIAL & BIOCHEMICAL RESEARCH AND TECHNOLOGIES
October 18-19, 2017
Toronto, Canada
International Congress on
&
Detection of carbapenem resistant Gram-Negative Bacilli from infected wounds in Khartoum
state-2014
Reem AbdElmoniem Dahab Khalil
University of Medical Sciences and Technology, Sudan.
Background:
Carbapenem family are from the recently synthesized beta-lactam
antibiotics which used as last resort antibiotics for treating infections caused by
multidrug-resistant Gram-negative bacilli and the resistant to them by Gram-negative
bacilli have been developed , due to production of variety of carbapenemase enzymes
and other mechanisms that significantly limits treatment options for life-threatening
infections.
Objective:
This study aims to detect carbapenem resistant Gram-negative rods from
infected wounds in Khartoum state and the production of carbapenemase enzymes by
the resistant isolates using phenotypic methods.
Method:
100 wound swabs were collected. All samples were cultured directly on blood
and MacConkey agar, Cultures were examined macroscopically and microscopically,
different standard biochemical tests were performed for identification of Gram-
negative bacilli. Standard antimicrobial susceptibility testing to Meropenem antibiotic
was done for all Gram-negative bacilli isolates, and Modified Hodge test was performed
for the resistant isolates.
Results:
77 Gram-negative bacilli were isolated from 100 samples, the commonest
pathogenic isolates were Proteus species (28%) followed by Klebsiella species (24%),
Escherichia coli(20%), Pseudomonas species (17%), Enterobacter species(10%) and
Acinetobacter species(1%). 13% of the isolates were Carbapenem resistant, and 50%
of the resistant isolates were positive for carbapenemase enzymes production using
Modified Hodge Test.
Conclusion:
the percentage of Carbapenem resistance is high. Pseudomonas species
followed by Escherichia coli were the most carbapenemase producers. Modified
Hodge test is simple method for detection of carbapenemase enzymes that can
detect many types of carbapenemase but not all types and it does not specify the
types. Further studies should be performed using larger sample size and other specific
methods especially PCR.
Speaker Biography
Reem AbdElmoniem Dahab Khalil is a 24years old medical laboratory specialist
(microbiologist), studied at UMST, Sudan and completed the master degree by the age
of 23,both by excellent degree, and cGPA 4.85 out of 5 in the BSc. Now she is a lecturer
in International University of Africa, Sudan and at the same time working at a hospital,
she is a beginner researcher, and she is very interested in the research work, especially
antimicrobials.
e:
reemdahab93@gmail.com