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Microbiology: Current Research 2017 | Volume 1, Issue 2
Joint Conference
GLOBAL APPLIED MICROBIOLOGY CONFERENCE
MICROBIAL & BIOCHEMICAL RESEARCH AND TECHNOLOGIES
October 18-19, 2017
Toronto, Canada
International Congress on
&
Molecular and phenotypical characterization of
Mannheimia haemolytica
isolated from goats in
Baghdad province
Waffa A Ahmed
Baghdad University, Iraq
M
annheimia haemolytica
(
M. haemolytica
) is a Gram-
negative bacterium, which can infect humans and
animals. It’s commensal as a normal flora of the nasopharynx
and tonsils in cattle, sheep and goats, pneumonic
pasterellosis is one of the most economically important
infectious disease in goats with a worldwide prevalence. This
study aimed to investigate the incidence of
M. haemolytica
by bacteriological and molecular characterization in goats.
One hundred nasopharyngeal swabs were collected
from apparently healthy farm goats, seven lung tissue
specimens and five nasal mucus swabs from slaughtered
goats in Baghdad. All samples were cultured on Blood and
MacConkey agars. Biochemical tests and EPI20E kit were
used for identification of the suspected colonies. 5(4.46%)
isolates of
M. haemolytica
were identified phenotypically
and confirmed diagnosis by polymerase chain reaction
(PCR) technique using two primers 16s rRNA and 12s rRNA
genes. The results of this study concluded that identification
of
M. haemolytica
by PCR was in accordance with those of
phenotypic test and use of more than primer could improve
identification of isolates.
Speaker Biography
Waffa A Ahmed is from the University of Baghdad. She has worked as the head of
Department of Quality Control, Veterinary State Company, Ministry of Agriculture,
(2005-2006). She has worked for eight years as Assistant Professor and Scientific
Researcher in Unit of Zoonotic researches, (2006-2014), also in Department of
Microbiology (2014-2017), College of Vet. Medicine, University of Baghdad. She had
participated in several scientific conferences in Iraq and global conferences in USA,
Canada, Germany and Egypt. Also, she had many invitations from many conferences.
She has participated in many examining committees for Post-graduate students in
Baghdad University and other universities in Iraq. She has taught more than seven
courses for Under and Post-graduate students (Diploma, MSc and PhD) in pathology,
microbiology, and zoonotic diseases in college of Vet. Med. She has published more
than 30 papers in reputed journals and has been serving as an Editorial Board Member
of Research Journal of Biology, and reviewed more than 100 articles in about seven
journals and more than 20 theses (scientific evaluation).
e:
waffaabduleah@yahoo.com