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Microbiology: Current Research 2017 | Volume 1, Issue 2
Joint Conference
GLOBAL APPLIED MICROBIOLOGY CONFERENCE
MICROBIAL & BIOCHEMICAL RESEARCH AND TECHNOLOGIES
October 18-19, 2017
Toronto, Canada
International Congress on
&
Isolation and characterization of oil degrading bacteria from contaminated soil at oil ARZEW refinery
Khadidja Senouci-Rezkallah
1
, Fatiha Dilmi
1, 2
and
Abdelwaheb Chibani
2
1
Université Mustapha Stambouli de Mascara, Algeria
2
University of Mostaganem, Algeria
B
iodegradation by indigenous bacteria represents one
of the primary mechanisms by which oil pollutants
can be removed from the environment. The aim of our
study is the isolation and identification of petroleum
hydrocarbon degrading bacteria from oil contaminated
soil samples. The samples were collected from differences
sites at Arzew refinery Northern Algeria. Bacteriological
diagnosis of soils studied corresponding to a biomass 14,
02. 107 CFU/g of soil in the lower polluted soil sample.
This biodiversity is inversely proportional to the increase
in oil content. Indeed, in the highly contaminated sample,
with a biomass of 9.3 .104 CFU/g of soil. The hydrocarbon
degrading bacteria isolated and identified belonged to
the following genera,
Pseudomonaceae, Bacilliaceae and
Staphylococcaceae. Biochimical tests revealed the presence
of Pseudomona aeroginosa, Pseudomona fluorecens,
pseudomona putida, pseudomona citronéllolis, Pseudomona
luteola, pseudomona fluorescens biovare 1, pseudomona
fluorescens biovare 3, pseudomona fluorescens biovare 5,
Bacillus sp, Staphylococcus hémolytique, Staphylococcus
hominis
. The ability of isolates to degrade the crude oil was
performed by gravimetric analysis. The biodegradation rate
of crude oil by
Pseudomonas aeruginosa
is the best with
82.7%, whereas the lower potential of degradation showed
in
Staphylococcus hominis
with 46.63 %. Among the existing
strains,
Pseudomonas aeruginosa
have the best production
of biosurfactants that reducing the surface tension of culture
medium until 19 mN/m, with an emulsion index of 22.72%,
and the area of oil displacement (0.9 cm). The strains
isolated are capable to produce a biosurfactants that has a
great power in the remobilization of hydrocarbons and the
acceleration of their biodegradation.
Speaker Biography
Fatiha Dilmi received the license (DES) degree from Mustapha Stambouli University,
Biology department, Mascara, Algeria in 2005, Master’s Degree in Biology from
Department of Biology, Faculty of Sciences, AL al- Bayt University in 2009, Jordan.
From 2009 till now, she was worked as assistant professor-researcher at Mustapha
Stambouli University, Mascara, Algeria. She worked on the isolation, characterization
and biodegradation ability of hydrocarbon degrading bacteria from contaminated
soil in petrol station for preparation of phD thesis. She is a member in Laboratory of
Microbiology and Plant Science, Department of Biology, Faculty of Natural and Life
Sciences, University of Abdelhamid Ibn Badis, Mostaganem, Algeria and Laboratory
for Research on Biological Systems and Geomatics (L.R.S.B.G), Department of Biology,
Faculty of Natural and Life Sciences, Mustapha Stambouli University, Mascara, Algeria.
e:
fatiha_dilmi@yahoo.com