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allied
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Microbiology: Current Research 2017 | Volume 1, Issue 2
Joint Conference
GLOBAL APPLIED MICROBIOLOGY CONFERENCE
MICROBIAL & BIOCHEMICAL RESEARCH AND TECHNOLOGIES
October 18-19, 2017
Toronto, Canada
International Congress on
&
The endophytes ofmedicinal plants are largely underexplored
despite their potential as repositories of bioactive
compounds including natural antioxidants. The purpose
of this study was to evaluate the total antioxidant capacity
(TAC) of ethyl acetate extracts of endophytic fungi isolated
from the medicinal plants
Kigelia africana
(
Penicillium
species
),
Annona senegalensis
(
Epicoccum sorghinum
) and
Vitex payos
(
Epicoccum nigrum
). The TPC was determined
using the Folin-Ciocalteu method. The OxiSelect™ total
antioxidant capacity assay kit was used to evaluate the
TAC. Fourier transform infrared (FTIR) analysis of the crude
extracts was also done to determine the possible different
functional groups present in the extracts. The extract
obtained from the endophyte
Epicoccum sorghinum
isolated
from
Annona senegalensis
demonstrated both the highest
total phenolic content (28.85±1.14mg GAE/g dry weight) and
total antioxidant capacity (593.46±1.86 μM CRE). A strong
positive linear correlation (r=0.95717) was found between
total antioxidant capacity and total phenolic content of
the tested crude extracts. The FT-IR spectral analysis of the
crude extracts confirmed the presence of molecules carrying
bonded hydroxyl (-OH) functional group characteristic of
phenolic compounds. The preliminary results indicated that
the studied endophytic fungi produced metabolites with
potential as sources of natural antioxidants and that the TPC
influences TAC.
e:
sibandaep@gmail.comAntioxidant activity of fungal endophytes isolated from
Kigelia africana, Annona senegalensis
and
Vitex payos
Edson P Sibanda
1, 2
, Musa Mabandla
2
and
Takafira Mduluza
2, 3
1
Scientific and Industrial Research and Development Centre, Zimbabwe
2
University of KwaZulu Natal, South Africa
3
University of Zimbabwe, Zimbabwe