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Microbiology: Current Research 2017 | Volume 1, Issue 2
Joint Conference
GLOBAL APPLIED MICROBIOLOGY CONFERENCE
MICROBIAL & BIOCHEMICAL RESEARCH AND TECHNOLOGIES
October 18-19, 2017
Toronto, Canada
International Congress on
&
Isolation of nontuberculous mycobacteria in the waters of a hemodialysis center
Soheila Khaghani, Seyed Mohammad Alavi and Shokouh Ghafari
Ahvaz Jundishapur University of Medical Sciences, Iran
Introduction & Aim:
Hemodialysis is a therapeutic manner for
chronic renal incompetence patients. The use of poorly treated
water during hemodialysis may lead to contamination with
nontuberculous mycobacteria (NTM). The aim of this study was
to investigate contamination in waters of a hemodialysis center
with nontuberculous mycobacteria (NTM).
Methods:
A total of 60 samples taken at different points in each
hospital’s hemodialysis distribution system were collected in
Ahvaz, Iran. A volume of 500 mLof the samples were ltered
through membrane lters with pores 0.45 mm in diameter.
Sediment of each sample was inoculated into two Lowenstein-
Jensen medium. Isolated mycobacteria colonies were studied
with phenotypic tests, PCR- restriction enzyme analysis (PRA)
and rpoB gene sequence analysis.
Results:
M. fortuitum, M. gordonae, M. lentiflavum
and
M.
moriokaense
were the most isolated NTM in the waters of
hemodialysis.
Discussion:
M. lentiflavum
has mainly clinical importance
in young children with cervical lymphadenitis and in
immunocompromised patients.
M. moriokaense
first isolated
from sputum of a patient with tuberculosis and from soil in
Morioka, Japan. Despite its nonvirulent nature, there have
been reports of clinically significant diseases caused by
M.
gordonae
, including disseminated infections urogenital tract
diseases, gastrointestinal tract infections, soft tissue damage,
and respiratory and pulmonary infections.
Conclusion:
This result demonstrates that dialysis water can
be storage of transmission of potential NTM pathogenic
among patients with weakened immunity. Suitable monitoring
to ensure the best control over the dialysis water system is
recommended.
Speaker Biography
Soheila khaghani is working in Infectious and Tropical Diseases Research Center, Health
Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz. She is
from Iran.
e:
soheilakhaghani44@gmail.com