analytical-chemistry-2019-keynote

1 / 3

Analytical Chemistry 2019
Page 11
November 20-21, 2019 | Berlin, Germany
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
YEARS
ANALYTICAL CHEMISTRY AND
CHROMATOGRAPHY METHODS
2nd International Conference on
ANALYTICAL CHEMISTRY IN RESEARCH ON
NICOTINIC RECEPTOR INTERACTIONS WITH
NEUROTOXIC PEPTIDES AND PROTEINS
Authors work is in the field of neurobiology and neurochemistry, close to
the analytical chemistry, the topic of this conference. Their main targets
are nicotinic acetylcholine receptors (nAChR). Invaluable instruments for
nAChR research are snake venom, α-neurotoxins and in proteomic studies
of venoms they recently found a new variant (αδ-bungarotoxin) of classical
α-bungarotoxin which inhibits muscle-type and neuronal α7 nAChRs, but
more reversibly. Another result of proteomics was covalently bound dimeric
α-cobratoxin. Computer modelling, peptide synthesis, analytical chemistry
and mass-spectrometry’s allowed as to obtain α-conotoxin PnIA analogues
which for the neuronal α7 nAChR had a 50-fold higher affinity than PnIA itself.
Neurotoxic peptides and proteins interacting with nAChRs provide informa-
tion about their binding sites necessary for drug design against neurodegen-
erative diseases, pain and inflammatory processes. For understanding physi-
ological processes and drug design, of great importance are human proteins
having the same three-finger folding as snake venom α-neurotoxins. Some
of them, like Lynx1 and SLURP-1, are localized in the brain and in the immune
system close to nAChRs and modulate their assembly and functioning. An il-
lustration of matching the analytical chemistry with other modern approach-
es is our recent work with Australian researchers who prepared SLURP-1 (81
amino-acid residues, 5 disulfides) by total chemical synthesis. It had the same
NMR structure as recombinant SLURP-1 (having an extra N-terminal Met
residue), but by combination of radioligand analysis, calcium imaging and
electrophysiology they demonstrated that this difference resulted in the se-
lectivity shift from α7 to α9/α10 nAChR.
Tsetlin V, J Chem Tech App 2019, Volume 3
Tsetlin V
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry- Russian
Academy of Sciences, Russia
Tsetlin V did his Ph D Degree in Chemistry (1973)
at the Shemyakin-Ovchinnikov Institute; Head
of the Department for molecular neuroimmune
signaling; Professor (1996); Corresponding Mem-
ber of the Russian Academy of Sciences (2006).
He was honored with Russian State Prize in Sci-
ence and Technology (1985) and the Humboldt
Prize (1992). He is an Invited Scientist at Imperial
College, London (1983-1984), Institute of Protein
Research, Osaka (1992-1993), Free University of
Berlin (1993-1994). He is the author of over 250
papers, including those in PNAS, Neuron, Nature
Str. Mol. Biol., Member of the FEBS J Advisory
Board (2000-2011), Biochem. J. (2013 - present).
His Citation Index is 4280 and Hirsh index 34.
BIOGRAPHY
Keynote Forum | Day 1
Journal of Chemical Technology and Applications | Volume 3
References
1. Utkin et al. Tsetlin VI. Biochem J. 2019,
476:1285.
2. Osipov et al. Tsetlin VI. J Biol Chem. 2012,
2876725
3. Kasheverov et al. Tsetlin VI.Sci Rep. 2016,
6:36848.
4. Tsetlin VI Trends Pharmacol Sci. 2015
Feb;36(2):109-23.
5. Durek et al. Tsetlin VI. Sci Rep. 2017,
7(1):16606.