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November 20-21, 2019 | Berlin, Germany
OF EXCELLENCE
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ANALYTICAL CHEMISTRY AND
CHROMATOGRAPHY METHODS
2
nd
International Conference on
Analytical Chemistry 2019
LC-MS/MS BASED PHYTOCHEMICAL FINGERPRINTING OF MALAYSIAN HERBS
Lee Suan Chua
Universiti Teknologi Malaysia, Malaysia
M
ajority of Malaysians apply herbs or herbal products in their daily life, solely based on the traditional
knowledge and practices. Many herbal products have recently been developed and promoted in the
market for the convenience of consumers to enhance their overall well-being. This includes
Eurycoma longi-
folia
(King of herb),
Labisia pumila
(Queen of herb) and other popular herbs such as
Orthosiphon aristatus
,
Andrographis paniculata
and
Ficus deltoidea
. The functionality of the herbal products is strongly related to their
phytochemicals which are very complex in nature. Therefore, it is important to have an unbiased and reliable
technique to identify or authenticate the products, especially those products with functional claims. Phyto-
chemical fingerprinting using the hyphenated technique of LC-MS/MS is suggested to be a comprehensive
analytical platform because it is sensitive enough even for trace quantity of phytochemicals. Phytochemicals
are secondary metabolites which usually possess certain biological functions in the defense mechanism of
plants. This explains the ethnopharmacological properties of herbal plants. The complex mixture of plant sam-
ples is separated by liquid chromatography, and individually flowed into mass spectrometer for ionization and
detection. Huge dataset that are generated during fingerprinting could be statistically analyzed using chemo-
metric tools for sample classification and identification. High performance and unsupervised multivariate data
analysis techniques such as principal component analysis, hierarchical clustering analysis and heat mapping
are the common pattern recognition tools for quality control and standardization of herbal samples. Therefore,
phytochemical fingerprinting coupled with chemometrics is a reliable technique to describe the entirety of
phenotypes for quality control and quality assurance of herbal samples.
J Chem Tech App 2019, Volume 3
Accepted Abstracts
Journal of Chemical Technology and Applications | Volume 3