Journal of Clinical and Bioanalytical Chemistry

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Short Article - Journal of Clinical and Bioanalytical Chemistry (2021) Volume 5, Issue 2

Simultaneous Determination of Four Index Polar Constitutes by HILIC-ESIMS/MS for the Quality Control of Guizhi Fuling Wan

Zhiran Zhang*

Shenyang Pharmaceutical University, CHINA

*Corresponding Author:
Zhiran Zhang
Shenyang Pharmaceutical University, CHINA

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Waters HILIC column (100 mm × 1.7 mm, 2.1 μm) with gradient elution using 0.1% formic acid aqueous solution (A) and acetonitrile (B) at a flow rate of 0.2 mL/min. All the compounds showed good linearity. The method provided good accuracy with perfect recoveries and precision, which has been successfully applied to the simultaneous identification and determination of 4 index compounds in a single run for the quality control of Guizhi- fu-ling Wan. Gui-zhi-fu-ling Wan (GFW) has been used in China for centuries. It is a traditional Chinese medical (Kampo) formulation that composed of five kinds of medicinal plants, Cinnamomum cassia BLUME (Cinnamomi Cortex), Paeonia lactiflora PALL (Peonies Radix), Paeonia suffruticosa ANDREWS (Moutan Cortex), Prunus persica BATSCH (Persicae Semen), and Poria cocos WOLF (Hoelen). GFW, prescript for treating blood disorders was compiled by Zhang Zhongjing, an outstanding physician in the Eastern Han Dynasty


A rapid and accurate method was developed and validated for the analysis of four index polar constitutes in Chinese medicinal preparation Gui-zhi-fu-ling Wan by microwave-assisted extraction coupled with hydrophilic interaction liquid chromatography-tandem mass spectrometry (MAE-HILIC - MS/MS). The chromatographic separation was performed on a Waters HILIC column (100 mm
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