Journal of Biotechnology and Phytochemistry

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.
Reach Us +44 7460731551

Short Communication - Journal of Biotechnology and Phytochemistry (2021) Volume 5, Issue 4

Microfluidic double emulsion and particle for a controlled release of therapeutic peptide - Marine Truchet - ESPCI, France

Marine Truchet*

ESPCI, France

*Corresponding Author:
Marine Truchet
ESPCI
France
E-mail: [email protected]

Accepted date: December 10, 2021

Visit for more related articles at Journal of Biotechnology and Phytochemistry

Abstract

Encapsulation of peptide therapeutics has a growing significance in pharmaceutical and biotech industry to control the release over weeks or months. If the capsule is made from biodegradable polymer, it will degrade slowly and release the drug progressive. To fabricate the particle, people often use the double emulsion/solvent removal method. A double emulsion of water in oil in water (W/O/W) is formed. The active ingredient is within the inner water droplet. The middle phase composed of the solvent and the biodegradable polymer is then solidified. The emulsification process is mostly performed with mechanical stirring or ultrasonication, a process that enables the creation of large volumes of emulsions but results in very bad monodispersity and low encapsulation yield. The production of monodispersed (W/O/W) double emulsions using different microfluidics technologies has been demonstrated. However, none of them could be used to prepare microparticles with desired properties compatible with control delivery of APIs. Each technique has one or several limitations: compatibility of chips with solvents, low throughput, droplet size. In this study, we solved these problems by combining traditional technologies with microfluidics. Our three-step hybrid process allows the formation of very well controlled size, morphology and drug loading particles. We managed, for the first time, to obtain micro-particles with desired particle size, whose structure is controlled and adapted to the delivery challenge we address. We are now able to study precisely the mechanism of release and the influence of each parameter on the drug release.

References

1. Jeong, H.H.; Issadore, D.; Lee, D. Recent developments in scale-up of microfluidic emulsion generation via parallelization. Korean J. Chem. Eng. 2016, 33, 1757–1766.
2. Mhatre, S.; Thaokar, R. Electrocoalescence in non-uniform electric fields: An experimental study. Chem. Eng. Process. Process Intensif. 2015, 96, 28–38.
3. Wan, Y.; Cao, X.; Zhang, S.; Wang, S.; Wu, Q. Fibrous poly(chitosan-g-DL-lactic acid) scaffolds prepared via electro-wet-spinning. Acta Biomater. 2008, 4, 876–886.
4. Saeki, D.; Sugiura, S.; Kanamori, T.; Sato, S.; Ichikawa, S. Microfluidic preparation of water-in-oil-in-water emulsions with an ultra-thin oil phase layer. Lab Chip 2010, 10, 357–362
5. Utada, A.S.; Chu, L.; Link, D.R.; Holtze, C.; Weitz, D.A. Dripping, jetting, drops, and wetting: The magic of microfluidics. MRS Bull. 2007, 32, 702–708.

 

Get the App