Research Article - Journal of Invasive and Non-Invasive Cardiology (2018) Volume 1, Issue 3
A comparison between microsurgery and standard coronary bypass technique of long term patency rates of Saphenous vein grafts
- *Corresponding Author:
- Spagnolo S, MD
Cardiovascular Department - Istituto Clinico Ligure di Alta SpecialitÓ (ICLAS) ? Rapallo (Genoa) GVM Care & Research, Italy
Tel: 203 974 7892
E-mail: [email protected]
Accepted date: November 21, 2018
Citation: Spagnolo S, Barbato L, Scalise F, et al. A comparison between microsurgery and standard coronary bypass technique of long term patency rates of Saphenous vein grafts. J Invasive Noninvasive Cardiol. 2018;1(3):6-11.
Introduction: To improve patency rates in coronary bypass grafting we borrowed from microsurgery the use of the operating microscope allowing a suturing technique that minimizes anastomosis restriction. When compared to the standard technique, microsurgery afforded considerably greater long term vein grafts patency rates. Method: Approximately 50% of venous bypass grafts are occluded within 10 years and recent investigations have shown 30% and 40% occlusion rates respectively 12 and 18 months after the operation. Among the factors accounting for coronary bypass dysfunction and occlusion, defective sutures causing stenosis of the distal anastomosis have been shown to play a relevant role. Based on this consideration, since the early nineties we applied a microsurgical technique to all patients undergoing coronary bypass surgery. In order to evaluate the results obtained with this approach, we compared long term patency rates in two groups of patients who had been previously submitted to coronary bypass grafting by either microsurgery or by the traditional technique. Results: From January 2000 to December 2008, 388 patients who had been submitted to coronary bypass surgery in our or other institutions underwent repeat coronary angiography at the Policlinico di Monza (Italy). In 239 patients (62%) Coronary Artery Bypass Graft (CABG) had been performed in other hospitals by the “standard” technique while in the 149 patients operated at the Policlinico di Monza (38%) revascularization had been completed by the microsurgical approach. Respectively 346 (52%) of the 666 grafts performed with the “standard” technique and 297 of the 354 (83%) grafts performed by microsurgery were patent. Regardless of the conduit used, patency rates were invariably greater when microsurgery was employed. However, due to the relatively limited size of the study population, the difference between the techniques reached statistical significance only when vein grafts were compared. In fact, in the two groups vein grafts patency rates were 38% and 81% respectively (p<0.005). Conclusion: Especially for vein grafts, microsurgery results in significantly greater long term patency rates. Our findings suggest that surgical stenosis induced by inaccurate suturing technique may account for a significant proportion of graft occlusions. This observation bears important clinical implications and suggests that, especially when applied on smaller recipient vessels, distal anastomoses should be performed using microsurgical techniques.
Venous bypass grafts, Microsurgery, Revascularization, Coronary angiography, Arteriography.
Coronary artery bypass grafting is not a definitive operation especially when saphenous vein grafts are used. In fact, approximately 50% of venous conduits are occluded within 10 years and 39% reintervention rates have been reported within 8 years . More recent investigations, such as the Prague IV  and the Prevent IV  trials, assessed vein grafts patency rates 12 to 18 months after the index operation and showed that respectively 30% and 40% of vein grafts were occluded at the time of control angiography. 50% occlusion rates were observed if the bypass operation had been performed “off pump” .
The observation that patency rates are significantly greater when arterial conduits are used , led to the established belief that the main causes of vein graft occlusion are: 1) the artero-venous diameter mismatch at the site of the distal anastomosis and; 2) the progressive development of vein graft disease caused by the increased pressure and shear forces imposed to the vein by the arterial circulation. In fact, while the difference in lumen diameter between coronary arteries and saphenous veins may be responsible for delayed flow velocity in the venous conduit and cause early thrombosis and occlusion , vein graft disease is more likely to cause graft failure in the long term .
A third and often neglected factor that also plays a relevant role in graft failure, however, is the presence of significant stenosis at the site of the distal anastomosis caused by inappropriate suturing technique. Obviously, the smaller the grafted vessel, the more the difficult in performing a proper anastomosis; and the rate of graft occlusion indeed appears to be inversely related to the size of the recipient vessel  .
This observation, along with the high patency rates observed in microsurgery , where the calibre of treated vessels is far smaller than the one of epicardial coronary arteries, led us to hypothesize that one major source of vein graft occlusion is the suturing technique and, since the early nineties, we reintroduced microsurgery in coronary venous by-pass grafting.
All this taken into consideration, it is clear that alternative techniques are strongly needed, to guarantee much better result in long term patency rates of the grafts, in particular when saphenous vein is used for surgery revascularization. Given this clinical need, years ago we started operating with the microsurgery technique described ahead and in figures. We had clear positive impressions, then at least we operated on hundreds of patients during the years. In the present article we report this experience of ours, that is, the results of a retrospective study that we conducted on a cohort of patients in whom coronary by-pass grafting was performed by microsurgery and were submitted to repeat coronary angiography for a variety of surgical indications. This cohort was compared with a group of patients undergone traditional bypass surgery that similarly presented later on to the hospital, with need of repeat coronary arteriography.
The aim of the study is definitely to assess, and then compare, by-pass patency rates in patients operated with traditional versus microsurgery technique, mainly focusing into the group of venous grafts, those that might benefit more from the use of microsurgery. Consequently, the precise outcome of the study is the rate of patency of the grafts, and not a clinical end point.
Patients and Methods
From January 2000 to December 2008, 388 patients who had been submitted to coronary bypass surgery in our or other institutions underwent repeat coronary angiography at the Policlinico di Monza department of cardiac surgery. In 239 patients (62%) CABG had been performed in other units by the “standard” technique while in the 149 patients operated by our team (38%) revascularisation had been completed with the microsurgical approach.
The need for readmission and repeat coronary angiography was dictated by a variety of cardiovascular conditions such as recurrence of angina, valvular disease, thoracic or abdominal aneurysms, and critical peripheral artery disease. In the two groups, the percent incidence of clinical conditions dictating readmission and cardiovascular surgery were similar (Table 1).
|Ischemic Heart Disease||76 (32%)||42 (29%)||ns|
|Valvular Disease||91 (38%)||54 (36%)||ns|
|Aneurysms||40 (17%)||31 (20%)||ns|
|Peripheral artery disease||31 (13%)||22 (15%)||ns|
Table 1: Reason of hospital readmission to perform repeat Coronary Arteriography.
Accordingly, there were no significant differences between the two groups, as regards age, sex, risk factors, previous Myocardial Infarction (MI), number of grafted vessels, type of conduits used and time elapsed from the initial operation (Table 2).
|Categories||Standard surgery||Microsurgery||P value|
|n 239 (62%)||n 149 (38%)|
|Age (years)||63 ± 16||61 ± 19||ns|
|Female gender (%)||46(19)||22 (14)||ns|
|Hypertension (%)||129(53)||85 (57)||ns|
|Previous MI (%)||111(46)||64(42)||ns|
|Years from previous surgery||6.7 ± 5.1||5.8 ± 4.3||ns|
|N. of grafts||666||354||-|
|N of grafts/pt.||2.7 ± 0,3||2.3 ± 0,5||ns|
|LIMA||197 (29)||130 (35)||ns|
|RIMA||24 (3.6)||12 (8)||ns|
|Radial artery||6 (1)||0||ns|
|Saphenous vein||439 (65)||212 (58)||ns|
Table 2:Patient?s characteristics: Comparison between standard vs. microsurgery.
These patients represent the population of our retrospective study. Given the characteristic of the study (retrospective, to evaluate a technique that clearly seems to improve surgical results, and that was used based on clinical judgement and when considered advantageous), there is no Ethical Committee request/approval for the study.
Since 1990 all patients undergoing coronary bypass surgery in the department of cardiovascular surgery of the Policlinico di Monza are operated with a microsurgical technique. In the initial phase of implementation, we employed surgical loupes enabling a 6x magnification factor: subsequently, we used the Leica M500 frontal operating microscope which allows up to 8x magnifications (Figure 1).
Compared to the traditional technique, high power magnification considerably reduces the amount of vessel wall involved in the suture and minimizes the distance between stitches. In fact, in agreement with the approach initially proposed by Carrel (Figure 2), the standard technique applies a continuous 7-8 zero suture and 8 mm gauge needles, with 3 stitches positioned at the two extremes and approximately 4 other stitches applied to the lateral aspects of the anastomosis.
Conversely, our approach employs 8 zero continuous suture and a smaller needle (5 mm gauge). The amount of vessel wall involved in the suture and the distance between two adjacent stitches is no more than 0.5 mm and the total number of stitches applied for each anastomosis varies from 30 to 40 (Figure 3). The average time required to perform a microsurgical anastomosis is approximately 15 min.
As already pointed out, the need for repeat coronary arteriography was dictated by the development of a variety of clinical situations that occurred after the initial by-pass operation. In the two groups, the time elapsed from surgery to the second admission was respectively 6.7 ± 5 and 5.8 ± 4 years (ns). In all study patients the investigation was carried out by the standard Judkins technique, using either the femoral or radial approach, as appropriate. For each coronary artery and grafts several projections, including craniocaudal angulations were performed, in order to visualize the full length of graft and recipient vessel and to optimize visualization of the site of the anastomosis.
Coronary angiograms were analysed in blind by two independent observers unaware of patients’ identity and type of operation they had received. Graft patency was established when both the graft and the native vessel were fully opacified and contrast run-off occurred with a grade 3 Thrombolysis in Myocardial Infarction (TIMI) flow (Figure 4).
Data are presented as number of patients and percentage in Tables 1 and 3, mean+1 standard deviation in Table 2. Differences in continuous variables were analyzed by the unpaired T test and Wilcoxon rank-sum test respectively for normally and non-normally distributed data.
|Conduit type||P value|
|Total grafts||Patent (%)||Total grafts||Patent (%)|
|LIMA||197||161 (81%)||130||117 (90%)||0.06|
|RIMA||24||14 (58%)||12||9 (75%)||0.27|
|Saphenous vein||439||168 (38%)||212||171 (81%)||< 0.001|
|Radial artery||6||3 (50%)||-|
Table 3: Patency rates subdivided by the conduit used for grafting, and by traditional vs. microsurgery technique.
Categorical variables were analyzed by the chi-square test with Yates correction, to compare patency rates of grafts of Left Internal Mammary Artery (LIMA) as well as grafts of saphenous vein. Regarding grafts in Right Internal Mammary Artery (RIMA), as numbers were small, we used the exact Fisher test with one degree of freedom. We used the Statcalcepiinfo statistical program.
There were no reasons to ask for an ethical allowance to adopt microvascular surgery instead of traditional methods, given that both surgeons and Hospital management did not fear any detrimental aspect of microsurgery vs. traditional approach to be investigated under ethical aspect or to be part of informed consent. Patients gave witnessed oral informed consent to the use of clinical data pertaining this investigation. Hospital files were analyzed after having been made anonymous.
The angiographic results are summarized in Table 3. Respectively, 346 (52%) of the 666 grafts performed with the “standard” technique and 297 of the 354 (83%) grafts performed by microsurgery were patent. Regardless of the conduit used, patency rates were invariably greater when microsurgery had been employed.
However, may be due to the relatively limited size of the study population, the difference between the two techniques reached statistical significance only when vein grafts were compared. Of the 651 venous conduits analyzed by angiography, 439 belonged to the group of patients operated in the standard fashion, while 212 were part of the microsurgery group. Of these grafts, respectively 168 (38%) and 171 (81%) were patent (p<0.001). (Figure 5)
Graft patency rates: traditional technique vs. microsurgery
The results of our study show that the use of microsurgery results in greater patency rates for all conduits employed for revascularization and that the difference is particularly evident and significant for vein grafts. Although arterial revascularization is currently recommended and represents the technique of choice for the left anterior descending coronary artery, vein grafts remain the most commonly used conduits for all other arteries . As stated by Sabik, “there are several reasons for this. First, because of their relatively large diameter and wall characteristics saphenous veins are easy to use; second, they provide plenty of vascular material and can therefore be used to perform multiple grafts; third, the saphenous vein is long and can reach virtually any coronary artery; fourth, it easy to harvest” .
Nevertheless, common clinical experience indicates that venous conduits invariably exhibit lower patency rates than arterial grafts and suggests that improvement in surgical techniques should be sought, in order to improve long term performance of these conduits . Arterial suture standard procedure, moreover, is usually better than the vessel one, and characterized also by a higher number of circumferential stitches.
As already pointed out in the introductory session of this article, it is common belief that veins occlude earlier and more frequently than arteries, essentially because of hydrodynamic and biological factors. Indeed, being larger than arteries, they tend to produce slower flow rates, especially when a large caliber mismatch between graft and recipient vessel is present. Also, being endowed with a thinner wall and muscular media, they are poorly equipped to sustain the increased pressure and shear forces typical of the arterial circulation, and tend to rapidly degenerate and to develop graft disease .
Even acknowledging the importance of these factors, the presence of significant stenosis at the site of the distal anastomosis is recognized to play a relevant role in graft failure, especially when the recipient vessel is small. Indeed, the rate of graft occlusion appears to be inversely related to the size of the grafted vessel  and the 10 year patency rate of vein grafts applied to the Left Anterior Descending (LAD) was 90% if the vessel calibre was 2 mm or greater and 52% when LAD diameter was less than 2 mm . All these considerations taken into account, we continue to think that microsurgery can be much better than traditional technique in vein graft, while the comparison with traditional arterial grafts and microsurgery is more uncertain.
“Surgical” stenosis as a cause of coronary graft occlusion
“Surgical” stenosis most commonly occur at the distal (coronary) end and surgical skill is an important factor in determining the fate of the graft. According to Poiseuille’s law, blood flow varies as a function of the fourth power of vessel diameter and even small changes in diameter due to technical inaccuracy, may affect flow rates in a major way.
Among the factors potentially determining surgical stenoses, the thickness of vessel wall involved in the suture and the distance between stitches appear very important . In fact, the more the thickness of vessel wall involved in the suture, the more the shortening of suture edges; the more the distance between two adjacent stitches, the more the reduction of vessel lumen (Figure 3). In a morphologic investigation of occlusive changes of 95 coronary anastomoses, Griffith et al.  found that stitches applied at 0.5 mm and 1.0 mm from the edge of vessels measuring 2 mm in diameter cause respectively 29% and 54 % reduction in cross-sectional area.
When applied to vessels of 1 mm, the reduction in crosssection is 52% and 90 %. The critical areas of a coronary anastomosis are the toe and the heel. An insufficient number of stitches in these two areas may cause constriction and lead to early or late stenosis. Furthermore, a running suture exerts a purse-string effect, that may be prevented by applying many small bites (15 to 20) that also serve to prevent stenosis of the inflow or outflow coronary artery . These observations led us to believe that the use of high magnification optical tools, as typically practiced in microvascular surgery, by allowing the use of smaller sutures, closer stitches, and less vessel wall, should minimize the likelihood of causing surgical stenosis (Figure 3).
History of microvascular surgery
The advances in the techniques and technology that made microsurgery popular began in the early 1960s, and the first report of a vascular intervention employing this technique for the repair of blood vessels was described by Jules Jacobson in 1960. Using an operating microscope, he coupled vessels as small as 1.4 mm and coined the term "microsurgery” . Based on his experience, Harold Kleinert and Mort Kasdan, hand surgeons at Louisville University, performed the first revascularization of a partial digital amputation in 1963 .
Contemporary reconstructive microsurgery was introduced by an American plastic surgeon, Dr. Harry J Buncke (1964) who reported a rabbit ear re-implantation, using a garage as operating theatre and home-made instruments . This was the first report of successful microsurgical intervention using blood vessels one millimeter in size. In 1966, Buncke used microsurgery to transplant a primate's great toe to its hand . During the late sixties and early 1970s, plastic surgeons ushered in many new microsurgical innovations that were previously unimaginable. The first human microsurgical transplantation of the big toe to thumb was performed in April 1968 by John Cobbett, in England [18,19]. In Australia Ian Taylor  developed new techniques to reconstruct head and neck cancer defects with living bone from the hip or the fibula. Although primarily developed and used by plastic surgeons, a number of surgical specialties now use microsurgical techniques.
In the early seventies, Green e Loop proposed the use of the operating microscope to perform coronary bypass surgery. The technique was soon abandoned by the majority of cardiac centres, mainly because of the longer times required to complete the anastomosis, and because of the difficulties in adapting the microscope position when performing anastomoses on the lateral and posterior walls of the heart. Our experience seems to disprove such perplexities as, in fact, we were able to apply the technique virtually to all recipient vessels, regardless of their epicardial position. Furthermore, microsurgery appeared particularly valuable on small vessels where the use of high power magnification and extremely thin sutures and needles, allowed to apply interrupted stitches on arteries of 1 mm diameter. Although performing a distal anastomosis by microsurgery implies a slightly longer time than that required when the traditional technique is used, the minor prolongation of ischemic times associated with this approach is easily compensated by adequate myocardial protection.
Finally, interrupted sutures have been held by some as the gold standard for coronary anastomoses  and Loop et al. reported that this technique was associated with the best long-term of internal mammary patency rates . They observed that interrupted suturing consistently produced minimal anastomosis deformities and concluded that interrupted sutures should be used when constructing anastomoses in small coronary arteries as shortening of a continuous suture may cause focal constriction. However, in spite of the theoretical superiority of the interrupted sutures, constructing anastomoses with continuous sutures has progressively gained popularity and has become standard practice in coronary surgery due to its advantages in terms of ease and operating speed.
Our results indicate that applying a microsurgical technique to coronary bypass surgery results in significantly greater long term patency rates. The technique appears particularly valuable when applied to vein grafts and to small recipient vessels.
Although the two study groups appear quite similar, as regards physical and clinical characteristics, our investigation is affected by the typical limitations of retrospective studies and by their potential selection biases. One clear bias is that our study population is selected by the clinical need of presenting to the hospital years after surgical revascularization, and then with clinical indication to repeat coronary angiography: thus, not all patients operated were reconsidered to re-evaluate the grafts (it was not planned a prospective study, this study is retrospective with the data obtainable following clinical indications).
Furthermore, while all patients belonging to the microsurgery group were operated in the same institution and with the same technique, those constituting the control group were operated in different centres, possibly employing different technical approaches. This certainly represents a further limitation of our study, but the patency rates of vein grafts that we observed in the control group are quite similar to those reported in the current literature. This suggests that the results obtained in this population are representative of “real world” bypass surgery.
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