The composition of the essential oil of Centaurea intricata Boiss. flowering aerial parts from Iran was analyzed for the first time using gas chromatography (GC) and gas chromatography–mass spectrometry (GC–MS). Thirty four constituents consisting 90.1% of the total oil were identified. The major ingredients were β-Caryophyllene (18.1%), Germacrene D (14.9%) and Caryophyllene oxide (11.8%). The volatile oil of Centaurea intricata Boiss. flowering aerial parts was characterized by the high content of sesquiterpene hydrocarbons (49.6%).