- Asian Journal of Biomedical and Pharmaceutical Sciences (2014) Volume 4, Issue 34
Synthesis and Characterization of Silver Nanoparticles of Insulin Plant (costus pictus D. Don) Leaves
Objectives: Cost effective and eco-friendly technique for green synthesis of silver nanoparticles from the methanolic extract of Costus pictus leaf and characterization of synthesized silver nanoparticles was carried out in this study.
Methods: Synthesis of silver nanoparticles of Insulin plant (Costus pictus) leaves was done by using 1mM AgNO3 solution and incubates 5hr at room temperature. Characterization of synthesized nanoparticles was done by UV–Vis absorption spectroscopy, SEM, polydispersity index and zeta value.
Results: The biological synthesis of silver nanoparticles using Costus pictus extract was shown to be rapid and produced particles of fairly uniform size and shape. As the methanolic leaf extracts of Costus pictus D. Don were mixed with the aqueous solution of silver nitrate, it changed into brown colour due to the excitation of surface Plasmon vibrations, which indicated the formation of methanolic extract of Costus pictus silver nanoparticles (MECPAgNPs). UV-Visible spectroscopy analysis of nanoparticles showed the broadening of the peak indicated the particles are poly dispersed. The surface Plasmon band in the silver nanoparticles in the solution remains close to 420nm. Throughout the reaction period indicating the particles are dispersed in the aqueous solution of silver nitrate, with no evidence for aggregation. The average particle size (z-average) was found to be 132.6nm, its polydispersity index was 0.248 and zeta values were measured and it was found to be -25.1mV with the peak area of 100% intensity. This indicates that the formed silver nanoparticle is stable. A SEM images showed that the silver nanoparticles formed were spherical in shape, with an average size of around 100nm. SEM showed uniformly distributed silver nanoparticles on the surface of the cells was observed.
Conclusion: The Biomedical application of silver nanoparticles can be rendered more effective by using biologically synthesized nanoparticles that are found to be exceptionally stable, and also minimize toxicity and cost.