Ethanol extracts of Dodonaea viscosa were used traditionally in India for the treatment of skin diseases. The present study was investigated for in vitro antimicrobial activity against pathogens namely Staphylococcus aureus, Bacillus subtilis, Streptococcus pyogenes, Pseudomonas auroginosa, Klebsiella pnemonia, Aspergillus níger, Trichoderma viride and Candida albicans using the agar well diffusion method. A parallel study was performed to identify the distribution and the concentration of the phytocompounds in the roots and leaves of this plant. For this purpose alcoholic extracts were prepared from each part of the plant and studied them separately. Among the leaf extracts of D. viscosa possess the highest inhibitory activity then the root extracts. The results revealed that the plant leaf extract possessed the highest inhibitory activity against the bacteria and fungi.